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Science 10 March 1995:
Vol. 267. no. 5203, pp. 1488 - 1491
DOI: 10.1126/science.7878467
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Articles

Science, Vol 267, Issue 5203, 1488-1491
Copyright © 1995 by American Association for the Advancement of Science

articles

Requirement of RNA polymerase III transcription factors for in vitro position-specific integration of a retroviruslike element

J Kirchner, CM Connolly, and SB Sandmeyer

Department of Microbiology and Molecular Genetics, University of California, Irvine 92717.

The yeast retroviruslike element Ty3 inserts at the transcription initiation sites of genes transcribed by RNA polymerase III (Pol III). An in vitro integration assay was developed with the use of Ty3 viruslike particles and a modified SUP2 tyrosine transfer RNA (tRNA(Tyr)) gene target. Integration was position-specific and required Ty3 integrase, Pol III transcription factor (TF) IIIB-, TFIIIC-, and Pol III-containing fractions showed that TFIIIB and TFIIIC, together, were sufficient for position-specific Ty3 integration, but not for transcription. This report demonstrates that in vitro integration of a retroelement can be targeted by cellular proteins.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
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R5 Retrotransposons Insert into a Family of Infrequently Transcribed 28S rRNA Genes of Planaria.
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Design of a nonviral vector for site-selective, efficient integration into the human genome.
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Mutational Analysis of the Transcription Factor IIIB-DNA Target of Ty3 Retroelement Integration.
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A Truncation Mutant of the 95-Kilodalton Subunit of Transcription Factor IIIC Reveals Asymmetry in Ty3 Integration.
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Targeting of the Yeast Ty5 Retrotransposon to Silent Chromatin Is Mediated by Interactions between Integrase and Sir4p.
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An active retrotransposon in Candida albicans.
N. J. Holton, T. J. D. Goodwin, M. I. Butler, and R. T. M. Poulter (2001)
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Protein Determinants of Insertional Specificity for the Drosophila Gypsy Retrovirus.
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Fission yeast retrotransposon Tf1 integration is targeted to 5' ends of open reading frames.
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Relationship between Retroviral DNA Integration and Gene Expression.
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J. Virol. 74, 8382-8389
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Involvement of DNA End-Binding Protein Ku in Ty Element Retrotransposition.
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The Integration Machinery of ZAM, a Retroelement from Drosophila melanogaster, Acts as a Sequence-Specific Endonuclease.
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J. Virol. 73, 7061-7064
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Modular Evolution of the Integrase Domain in the Ty3/Gypsy Class of LTR Retrotransposons.
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J. Virol. 73, 5186-5190
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Genome System Architecture and Natural Genetic Engineering in Evolution.
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Ann. N.Y. Acad. Sci. 870, 23-35
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Host Genes That Affect the Target-Site Distribution of the Yeast Retrotransposon Ty1.
H. Huang, J. Y. Hong, C. L. Burck, and S. W. Liebman (1999)
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A Preferred Target DNA Structure for Retroviral Integrase in Vitro.
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Yeast Ty1 Retrotransposition Is Stimulated by a Synergistic Interaction between Mutations in Chromatin Assembly Factor I and Histone Regulatory Proteins.
Z. Qian, H. Huang, J. Y. Hong, C. L. Burck, S. D. Johnston, J. Berman, A. Carol, and S. W. Liebman (1998)
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W.-Y. Hu and K. M. Derbyshire (1998)
J. Bacteriol. 180, 3039-3048
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Chromosome Structure and Human Immunodeficiency Virus Type 1 cDNA Integration: Centromeric Alphoid Repeats Are a Disfavored Target.
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J. Virol. 72, 4297-4307
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Structure-function analysis of integrase interactor 1/hSNF5L1 reveals differential properties of two repeat motifs present in the highly conserved region.
A. Morozov, E. Yung, and G. V. Kalpana (1998)
PNAS 95, 1120-1125
   Abstract »    Full Text »    PDF »
Silent chromatin determines target preference of the Saccharomyces retrotransposon Ty5.
S. Zou and D. F. Voytas (1997)
PNAS 94, 7412-7416
   Abstract »    Full Text »    PDF »
Conjugating plasmids are preferred targets for Tn7..
C A Wolkow, R T DeBoy, and N L Craig (1996)
Genes & Dev. 10, 2145-2157
   Abstract »    PDF »
The Saccharomyces retrotransposon Ty5 integrates preferentially into regions of silent chromatin at the telomeres and mating loci..
S Zou, N Ke, J M Kim, and D F Voytas (1996)
Genes & Dev. 10, 634-645
   Abstract »    PDF »
Targeting retroviral integration.
F Bushman (1995)
Science 267, 1443-1444
   PDF »
The Brf and TATA-binding Protein Subunits of the RNA Polymerase III Transcription Factor IIIB Mediate Position-specific Integration of the Gypsy-like Element, Ty3.
L. Yieh, G. Kassavetis, E. P. Geiduschek, and S. B. Sandmeyer (2000)
J. Biol. Chem. 275, 29800-29807
   Abstract »    Full Text »    PDF »
Recognition of triple-helical DNA structures by transposon Tn7.
J. E. Rao, P. S. Miller, and N. L. Craig (2000)
PNAS 97, 3936-3941
   Abstract »    Full Text »    PDF »


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Science. ISSN 0036-8075 (print), 1095-9203 (online)