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Science 24 February 1995: Vol. 267. no. 5201, pp. 1175 - 1178 DOI: 10.1126/science.7855600
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Articles
Science, Vol 267, Issue 5201, 1175-1178
Copyright © 1995 by American Association for the Advancement of Science
A regulatory role for ARF6 in receptor-mediated endocytosis
C D'Souza-Schorey,
G Li,
MI Colombo,
and
PD Stahl
Department of Cell Biology and Physiology, Washington University School of Medicine, St. Louis, MO 63110.
Adenosine diphosphate-ribosylation factor 6 (ARF6), ARF6 mutants, and ARF1 were transiently expressed in Chinese hamster ovary cells, and the effects on receptor-mediated endocytosis were assessed. Overexpressed ARF6 localized to the cell periphery and led to a redistribution of transferrin receptors to the cell surface and a decrease in the rate of uptake of transferrin. Similar results were obtained when a mutant defective in guanosine triphosphate hydrolysis was expressed. Expression of a dominant negative mutant, ARF6(T27N), resulted in an intracellular distribution of transferrin receptors and an inhibition of transferrin recycling to the cell surface. In contrast, overexpression of ARF1 had little or no effect on these parameters of endocytosis.
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- The Activation of Phospholipase D by Endothelin-1, Angiotensin II, and Platelet-Derived Growth Factor in Vascular Smooth Muscle A10 Cells Is Mediated by Small G Proteins of the ADP-Ribosylation Factor Family.
- K. Shome, M. A. Rizzo, C. Vasudevan, B. Andresen, and G. Romero (2000)
Endocrinology
141, 2200-2208
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- ADP-Ribosylation Factor 6 Regulates Actin Cytoskeleton Remodeling in Coordination with Rac1 and RhoA.
- R. L. Boshans, S. Szanto, L. van Aelst, and C. D'Souza-Schorey (2000)
Mol. Cell. Biol.
20, 3685-3694
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- GIT Proteins, A Novel Family of Phosphatidylinositol 3,4,5-Trisphosphate-stimulated GTPase-activating Proteins for ARF6.
- N. Vitale, W. A. Patton, J. Moss, M. Vaughan, R. J. Lefkowitz, and R. T. Premont (2000)
J. Biol. Chem.
275, 13901-13906
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- A New Paxillin-binding Protein, PAG3/Papalpha /KIAA0400, Bearing an ADP-Ribosylation Factor GTPase-activating Protein Activity, Is Involved in Paxillin Recruitment to Focal Adhesions and Cell Migration.
- A. Kondo, S. Hashimoto, H. Yano, K. Nagayama, Y. Mazaki, and H. Sabe (2000)
Mol. Biol. Cell
11, 1315-1327
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- ARF1 Regulates pH-dependent COP Functions in the Early Endocytic Pathway.
- F. Gu and J. Gruenberg (2000)
J. Biol. Chem.
275, 8154-8160
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- Multiple endocytic pathways of G protein-coupled receptors delineated by GIT1 sensitivity.
- A. Claing, S. J. Perry, M. Achiriloaie, J. K. L. Walker, J. P. Albanesi, R. J. Lefkowitz, and R. T. Premont (2000)
PNAS
97, 1119-1124
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- Identification of Arfophilin, a Target Protein for GTP-bound Class II ADP-ribosylation Factors.
- O.-H. Shin, A. H. Ross, I. Mihai, and J. H. Exton (1999)
J. Biol. Chem.
274, 36609-36615
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- Adp-Ribosylation Factor 6 and Endocytosis at the Apical Surface of Madin-Darby Canine Kidney Cells.
- Y. Altschuler, S.-H. Liu, L. Katz, K. Tang, S. Hardy, F. Brodsky, G. Apodaca, and K. Mostov (1999)
J. Cell Biol.
147, 7-12
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- Rab5 Induces Rac-independent Lamellipodia Formation and Cell Migration.
- M. Spaargaren and J. L. Bos (1999)
Mol. Biol. Cell
10, 3239-3250
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- ADP-ribosylation Factor 6 as a Target of Guanine Nucleotide Exchange Factor GRP1.
- S. E. Langille, V. Patki, J. K. Klarlund, J. M. Buxton, J. J. Holik, A. Chawla, S. Corvera, and M. P. Czech (1999)
J. Biol. Chem.
274, 27099-27104
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- ADP-ribosylation Factor 6 (ARF6) Defines Two Insulin-regulated Secretory Pathways in Adipocytes.
- C. Z. Yang and M. Mueckler (1999)
J. Biol. Chem.
274, 25297-25300
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- Distribution of ARF6 between Membrane and Cytosol Is Regulated by Its GTPase Cycle.
- J. Gaschet and V. W. Hsu (1999)
J. Biol. Chem.
274, 20040-20045
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- Evidence for a Role for ADP-ribosylation Factor 6 in Insulin-stimulated Glucose Transporter-4 (GLUT4) Trafficking in 3T3-L1 Adipocytes.
- C. A. Millar, K. A. Powell, G. R. X. Hickson, M.-F. Bader, and G. W. Gould (1999)
J. Biol. Chem.
274, 17619-17625
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- Structural Elements of ADP-ribosylation Factor 1 Required for Functional Interaction with Cytohesin-1.
- G. Pacheco-Rodriguez, W. A. Patton, R. Adamik, H.-S. Yoo, F.-J. S. Lee, G.-F. Zhang, J. Moss, and M. Vaughan (1999)
J. Biol. Chem.
274, 12438-12444
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- Structural basis for the inhibitory effect of brefeldin A on guanine nucleotide-exchange proteins for ADP-ribosylation factors.
- M. Sata, J. Moss, and M. Vaughan (1999)
PNAS
96, 2752-2757
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- Identification of a New Pyk2 Target Protein with Arf-GAP Activity.
- J. Andreev, J.-P. Simon, D. D. Sabatini, J. Kam, G. Plowman, P. A. Randazzo, and J. Schlessinger (1999)
Mol. Cell. Biol.
19, 2338-2350
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- Neurotransmitter Secretion along Growing Nerve Processes: Comparison with Synaptic Vesicle Exocytosis.
- S. Zakharenko, S. Chang, M. O'Donoghue, and S. V. Popov (1999)
J. Cell Biol.
144, 507-518
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- A presynaptic role for the ADP ribosylation factor (ARF)-specific GDP/GTP exchange factor msec7-1.
- U. Ashery, H. Koch, V. Scheuss, N. Brose, and J. Rettig (1999)
PNAS
96, 1094-1099
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- ARF6 requirement for Rac ruffling suggests a role for membrane trafficking in cortical actin rearrangements.
- H Radhakrishna, O Al-Awar, Z Khachikian, and J. Donaldson (1999)
J. Cell Sci.
112, 855-866
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