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Science 24 February 1995:
Vol. 267. no. 5201, pp. 1175 - 1178
DOI: 10.1126/science.7855600

Articles

Science, Vol 267, Issue 5201, 1175-1178
Copyright © 1995 by American Association for the Advancement of Science


articles

A regulatory role for ARF6 in receptor-mediated endocytosis

C D'Souza-Schorey, G Li, MI Colombo, and PD Stahl

Department of Cell Biology and Physiology, Washington University School of Medicine, St. Louis, MO 63110.

Adenosine diphosphate-ribosylation factor 6 (ARF6), ARF6 mutants, and ARF1 were transiently expressed in Chinese hamster ovary cells, and the effects on receptor-mediated endocytosis were assessed. Overexpressed ARF6 localized to the cell periphery and led to a redistribution of transferrin receptors to the cell surface and a decrease in the rate of uptake of transferrin. Similar results were obtained when a mutant defective in guanosine triphosphate hydrolysis was expressed. Expression of a dominant negative mutant, ARF6(T27N), resulted in an intracellular distribution of transferrin receptors and an inhibition of transferrin recycling to the cell surface. In contrast, overexpression of ARF1 had little or no effect on these parameters of endocytosis.


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A. Claing, S. J. Perry, M. Achiriloaie, J. K. L. Walker, J. P. Albanesi, R. J. Lefkowitz, and R. T. Premont (2000)
PNAS 97, 1119-1124
   Abstract »    Full Text »    PDF »
Identification of Arfophilin, a Target Protein for GTP-bound Class II ADP-ribosylation Factors.
O.-H. Shin, A. H. Ross, I. Mihai, and J. H. Exton (1999)
J. Biol. Chem. 274, 36609-36615
   Abstract »    Full Text »    PDF »
Adp-Ribosylation Factor 6 and Endocytosis at the Apical Surface of Madin-Darby Canine Kidney Cells.
Y. Altschuler, S.-H. Liu, L. Katz, K. Tang, S. Hardy, F. Brodsky, G. Apodaca, and K. Mostov (1999)
J. Cell Biol. 147, 7-12
   Abstract »    Full Text »    PDF »
Rab5 Induces Rac-independent Lamellipodia Formation and Cell Migration.
M. Spaargaren and J. L. Bos (1999)
Mol. Biol. Cell 10, 3239-3250
   Abstract »    Full Text »
ADP-ribosylation Factor 6 as a Target of Guanine Nucleotide Exchange Factor GRP1.
S. E. Langille, V. Patki, J. K. Klarlund, J. M. Buxton, J. J. Holik, A. Chawla, S. Corvera, and M. P. Czech (1999)
J. Biol. Chem. 274, 27099-27104
   Abstract »    Full Text »    PDF »
ADP-ribosylation Factor 6 (ARF6) Defines Two Insulin-regulated Secretory Pathways in Adipocytes.
C. Z. Yang and M. Mueckler (1999)
J. Biol. Chem. 274, 25297-25300
   Abstract »    Full Text »    PDF »
Distribution of ARF6 between Membrane and Cytosol Is Regulated by Its GTPase Cycle.
J. Gaschet and V. W. Hsu (1999)
J. Biol. Chem. 274, 20040-20045
   Abstract »    Full Text »    PDF »
Evidence for a Role for ADP-ribosylation Factor 6 in Insulin-stimulated Glucose Transporter-4 (GLUT4) Trafficking in 3T3-L1 Adipocytes.
C. A. Millar, K. A. Powell, G. R. X. Hickson, M.-F. Bader, and G. W. Gould (1999)
J. Biol. Chem. 274, 17619-17625
   Abstract »    Full Text »    PDF »
Structural Elements of ADP-ribosylation Factor 1 Required for Functional Interaction with Cytohesin-1.
G. Pacheco-Rodriguez, W. A. Patton, R. Adamik, H.-S. Yoo, F.-J. S. Lee, G.-F. Zhang, J. Moss, and M. Vaughan (1999)
J. Biol. Chem. 274, 12438-12444
   Abstract »    Full Text »    PDF »
Structural basis for the inhibitory effect of brefeldin A on guanine nucleotide-exchange proteins for ADP-ribosylation factors.
M. Sata, J. Moss, and M. Vaughan (1999)
PNAS 96, 2752-2757
   Abstract »    Full Text »    PDF »
Identification of a New Pyk2 Target Protein with Arf-GAP Activity.
J. Andreev, J.-P. Simon, D. D. Sabatini, J. Kam, G. Plowman, P. A. Randazzo, and J. Schlessinger (1999)
Mol. Cell. Biol. 19, 2338-2350
   Abstract »    Full Text »    PDF »
Neurotransmitter Secretion along Growing Nerve Processes: Comparison with Synaptic Vesicle Exocytosis.
S. Zakharenko, S. Chang, M. O'Donoghue, and S. V. Popov (1999)
J. Cell Biol. 144, 507-518
   Abstract »    Full Text »    PDF »
A presynaptic role for the ADP ribosylation factor (ARF)-specific GDP/GTP exchange factor msec7-1.
U. Ashery, H. Koch, V. Scheuss, N. Brose, and J. Rettig (1999)
PNAS 96, 1094-1099
   Abstract »    Full Text »    PDF »
ARF6 requirement for Rac ruffling suggests a role for membrane trafficking in cortical actin rearrangements.
H Radhakrishna, O Al-Awar, Z Khachikian, and J. Donaldson (1999)
J. Cell Sci. 112, 855-866
   Abstract »    PDF »



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