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Science 24 February 1995:
Vol. 267. no. 5201, pp. 1166 - 1169
DOI: 10.1126/science.7855597
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Articles

Science, Vol 267, Issue 5201, 1166-1169
Copyright © 1995 by American Association for the Advancement of Science

articles

A role for exonuclease I from S. pombe in mutation avoidance and mismatch correction

P Szankasi and GR Smith

Fred Hutchinson Cancer Research Center, Seattle, WA 98104.

Exonuclease I (Exo I) from Schizosaccharomyces pombe, a 5'-->3' double-stranded DNA exonuclease, is induced during meiotic prophase I. The exo1 gene is a member of a family of related DNA repair genes, including RAD2/rad13/xpgc and YKL510/rad2, conserved from yeast to humans. An exo1 mutant displays a mutator phenotype and alters activity of the ade6-M387 marker effect. These results suggest that Exo I acts in a pathway that corrects mismatched base pairs.


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ATR-dependent pathways control hEXO1 stability in response to stalled forks.
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Decreased Meiotic Intergenic Recombination and Increased Meiosis I Nondisjunction in exo1 Mutants of Saccharomyces cerevisiae.
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Mol. Cell. Biol. 19, 2000-2007
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Newly Discovered Archaebacterial Flap Endonucleases Show a Structure-Specific Mechanism for DNA Substrate Binding and Catalysis Resembling Human Flap Endonuclease-1.
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Mismatch Repair in Escherichia coli Cells Lacking Single-Strand Exonucleases ExoI, ExoVII, and RecJ.
R. S. Harris, K. J. Ross, M.-J. Lombardo, and S. M. Rosenberg (1998)
J. Bacteriol. 180, 989-993
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Strand-specific Mismatch Repair in Mammalian Cells.
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J. Biol. Chem. 272, 24727-24730
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Identification and characterization of Saccharomyces cerevisiae EXO1, a gene encoding an exonuclease that interacts with MSH2.
D. X. Tishkoff, A. L. Boerger, P. Bertrand, N. Filosi, G. M. Gaida, M. F. Kane, and R. D. Kolodner (1997)
PNAS 94, 7487-7492
   Abstract »    Full Text »    PDF »
Enzymes and Reactions at the Eukaryotic DNA Replication Fork.
R. A. Bambara, R. S. Murante, and L. A. Henricksen (1997)
J. Biol. Chem. 272, 4647-4650
   Full Text »    PDF »
Cleavage of Insertion/Deletion Mismatches, Flap and Pseudo-Y DNA Structures by Deoxyinosine 3'-Endonuclease from Escherichia coli.
M. Yao and Y. W. Kow (1996)
J. Biol. Chem. 271, 30672-30676
   Abstract »    Full Text »    PDF »
Biochemistry and genetics of eukaryotic mismatch repair..
R Kolodner (1996)
Genes & Dev. 10, 1433-1442
   PDF »
Replication Protein A Confers Structure-specific Endonuclease Activities to the XPF-ERCC1 and XPG Subunits of Human DNA Repair Excision Nuclease.
T. Matsunaga, C.-H. Park, T. Bessho, D. Mu, and A. Sancar (1996)
J. Biol. Chem. 271, 11047-11050
   Abstract »    Full Text »    PDF »
Binding and Repair of Mismatched DNA Mediated by Rhp14, the Fission Yeast Homologue of Human XPA.
M. Hohl, O. Christensen, C. Kunz, H. Naegeli, and O. Fleck (2001)
J. Biol. Chem. 276, 30766-30772
   Abstract »    Full Text »    PDF »
Identification of Mismatch Repair Protein Complexes in HeLa Nuclear Extracts and Their Interaction with Heteroduplex DNA.
N. Matton, J. Simonetti, and K. Williams (2000)
J. Biol. Chem. 275, 17808-17813
   Abstract »    Full Text »    PDF »
In vivo requirement for RecJ, ExoVII, ExoI, and ExoX in methyl-directed mismatch repair.
V. Burdett, C. Baitinger, M. Viswanathan, S. T. Lovett, and P. Modrich (2001)
PNAS 98, 6765-6770
   Abstract »    Full Text »    PDF »
Interactions of Exo1p with components of MutLalpha in Saccharomyces cerevisiae.
P. T. Tran, J. A. Simon, and R. M. Liskay (2001)
PNAS 98, 9760-9765
   Abstract »    Full Text »    PDF »


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