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Science 24 February 1995:
Vol. 267. no. 5201, pp. 1131 - 1137
DOI: 10.1126/science.7855590
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Articles

Science, Vol 267, Issue 5201, 1131-1137
Copyright © 1995 by American Association for the Advancement of Science

articles

Head-on collision between a DNA replication apparatus and RNA polymerase transcription complex

B Liu and BM Alberts

Department of Biochemistry and Biophysics, University of California, San Francisco 94143-0448.

An in vitro system reconstituted from purified proteins has been used to examine what happens when the DNA replication apparatus of bacteriophage T4 collides with an Escherichia coli RNA polymerase ternary transcription complex that is poised to move in the direction opposite to that of the moving replication fork. In the absence of a DNA helicase, the replication fork stalls for many minutes after its encounter with the RNA polymerase. However, when the T4 gene 41 DNA helicase is present, the replication fork passes the RNA polymerase after a pause of a few seconds. This brief pause is longer than the pause observed for a codirectional collision between the same two polymerases, suggesting that there is an inherent disadvantage to having replication and transcription directions oriented head to head. As for a codirectional collision, the RNA polymerase remains competent to resume faithful RNA chain elongation after the DNA replication fork passes; most strikingly, the RNA polymerase has switched from its original template strand to use the newly synthesized daughter DNA strand as the template.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Single-stranded DNA-binding Protein in Vitro Eliminates the Orientation-dependent Impediment to Polymerase Passage on CAG/CTG Repeats.
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Genome-wide coorientation of replication and transcription reduces adverse effects on replication in Bacillus subtilis.
J. D. Wang, M. B. Berkmen, and A. D. Grossman (2007)
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Replication Fork Stalling at Natural Impediments.
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A study on the correlation of nucleotide skews and the positioning of the origin of replication: different modes of replication in bacterial species.
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Interruptions in gene expression drive highly expressed operons to the leading strand of DNA replication.
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Mechanisms of Transcription-Replication Collisions in Bacteria.
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The Myotonic Dystrophy Type 1 Triplet Repeat Sequence Induces Gross Deletions and Inversions.
M. Wojciechowska, A. Bacolla, J. E. Larson, and R. D. Wells (2005)
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The replication-related organization of bacterial genomes.
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From The Cover: The dynamic processivity of the T4 DNA polymerase during replication.
J. Yang, Z. Zhuang, R. M. Roccasecca, M. A. Trakselis, and S. J. Benkovic (2004)
PNAS 101, 8289-8294
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Functional Uncoupling of Twin Polymerases: MECHANISM OF POLYMERASE DISSOCIATION FROM A LAGGING-STRAND BLOCK.
P. McInerney and M. O'Donnell (2004)
J. Biol. Chem. 279, 21543-21551
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Bacteriophage T4 Genome.
E. S. Miller, E. Kutter, G. Mosig, F. Arisaka, T. Kunisawa, and W. Ruger (2003)
Microbiol. Mol. Biol. Rev. 67, 86-156
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Developmental Changes in the Sciara II/9A Initiation Zone for DNA Replication.
V. V. Lunyak, M. Ezrokhi, H. S. Smith, and S. A. Gerbi (2002)
Mol. Cell. Biol. 22, 8426-8437
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Characterization of DNA synthesis catalyzed by bacteriophage T4 replication complexes reconstituted on synthetic circular substrates.
F. A. Kadyrov and J. W. Drake (2002)
Nucleic Acids Res. 30, 4387-4397
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RNA Polymerases from Bacillus subtilis and Escherichia coli Differ in Recognition of Regulatory Signals In Vitro.
I. Artsimovitch, V. Svetlov, L. Anthony, R. R. Burgess, and R. Landick (2000)
J. Bacteriol. 182, 6027-6035
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DnaB Helicase Is Unable to Dissociate RNA-DNA Hybrids. ITS IMPLICATION IN THE POLAR PAUSING OF REPLICATION FORKS AT ColE1 ORIGINS.
D. Santamaria, G. de la Cueva, M. L. Martinez-Robles, D. B. Krimer, P. Hernandez, and J. B. Schvartzman (1998)
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Mechanistic Studies on the Impact of Transcription on Sequence-specific Termination of DNA Replication and Vice Versa.
B. K. Mohanty, T. Sahoo, and D. Bastia (1998)
J. Biol. Chem. 273, 3051-3059
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The ColE1 Unidirectional Origin Acts as a Polar Replication Fork Pausing Site.
E. Viguera, P. Hernandez, D. B. Krimer, A. S. Boistov, R. Lurz, J. C. Alonso, and J. B. Schvartzman (1996)
J. Biol. Chem. 271, 22414-22421
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Positive Torsional Strain Causes the Formation of a Four-way Junction at Replication Forks.
L. Postow, C. Ullsperger, R. W. Keller, C. Bustamante, A. V. Vologodskii, and N. R. Cozzarelli (2001)
J. Biol. Chem. 276, 2790-2796
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Science. ISSN 0036-8075 (print), 1095-9203 (online)