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Science 17 February 1995:
Vol. 267. no. 5200, pp. 1022 - 1024
DOI: 10.1126/science.7863328

Articles

Science, Vol 267, Issue 5200, 1022-1024
Copyright © 1995 by American Association for the Advancement of Science


articles

Inhibition of myogenic differentiation in proliferating myoblasts by cyclin D1-dependent kinase

SX Skapek, J Rhee, DB Spicer, and AB Lassar

Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115.

Although the myogenic regulator MyoD is expressed in proliferating myoblasts, differentiation of these cells is limited to the G0 phase of the cell cycle. Forced expression of cyclin D1, but not cyclins A, B, or E, inhibited the ability of MyoD to transactivate muscle-specific genes and correlated with phosphorylation of MyoD. Transfection of myoblasts with cyclin-dependent kinase (Cdk) inhibitors p21 and p16 augmented muscle-specific gene expression in cells maintained in high concentrations of serum, suggesting that an active cyclin-Cdk complex suppresses MyoD function in proliferating cells.


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J. Biol. Chem. 272, 33327-33337
   Abstract »    Full Text »    PDF »
Regulation of Distinct Stages of Skeletal Muscle Differentiation by Mitogen-Activated Protein Kinases.
A. M. Bennett and N. K. Tonks (1997)
Science 278, 1288-1291
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Biphasic Regulation of Breast Cancer Cell Growth by Progesterone: Role of the Cyclin-Dependent Kinase Inhibitors, p21 and p27Kip1.
S. D. Groshong, G. I. Owen, B. Grimison, I. E. Schauer, M. C. Todd, T. A. Langan, R. A. Sclafani, C. A. Lange, and K. B. Horwitz (1997)
Mol. Endocrinol. 11, 1593-1607
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A Novel, Nerve Growth Factor-activated Pathway Involving Nitric Oxide, p53, and p21WAF1 Regulates Neuronal Differentiation of PC12 Cells.
W. Poluha, C. M. Schonhoff, K. S. Harrington, M. B. Lachyankar, N. E. Crosbie, D. A. Bulseco, and A. H. Ross (1997)
J. Biol. Chem. 272, 24002-24007
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Oligodendrocyte precursor differentiation is perturbed in the absence of the cyclin-dependent kinase inhibitor p27Kip1.
P. Casaccia-Bonnefil, R. Tikoo, H. Kiyokawa, V. Friedrich Jr., M. V. Chao, and A. Koff (1997)
Genes & Dev. 11, 2335-2346
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PPARgamma induces cell cycle withdrawal: inhibition of E2F/DP DNA-binding activity via down-regulation of PP2A.
S. Altiok, M. Xu, and B. M. Spiegelman (1997)
Genes & Dev. 11, 1987-1998
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