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Science 10 February 1995:
Vol. 267. no. 5199, pp. 883 - 885
DOI: 10.1126/science.7846531

Articles

Science, Vol 267, Issue 5199, 883-885
Copyright © 1995 by American Association for the Advancement of Science


articles

Synergistic roles for receptor occupancy and aggregation in integrin transmembrane function

S Miyamoto, SK Akiyama, and KM Yamada

Laboratory of Developmental Biology, National Institute of Dental Research, National Institutes of Health, Bethesda, MD 20892-4370.

Integrin receptors mediate cell adhesion, signal transduction, and cytoskeletal organization. How a single transmembrane receptor can fulfill multiple functions was clarified by comparing roles of receptor occupancy and aggregation. Integrin occupancy by monovalent ligand induced receptor redistribution, but minimal tyrosine phosphorylation signaling or cytoskeletal protein redistribution. Aggregation of integrins by noninhibitory monoclonal antibodies on beads induced intracellular accumulations of pp125FAK and tensin, as well as phosphorylation, but no accumulation of other cytoskeletal proteins such as talin. Combining antibody-mediated clustering with monovalent ligand occupancy induced accumulation of seven cytoskeletal proteins, including alpha-actinin, talin, and F-actin, thereby mimicking multivalent interactions with fibronectin or polyvalent peptides. Integrins therefore mediate a complex repertoire of functions through the distinct effects of receptor aggregation, receptor occupancy, or both together.


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V. Petit, B. Boyer, D. Lentz, C. E. Turner, J. P. Thiery, and A. M. Valles (2000)
J. Cell Biol. 148, 957-970
   Abstract »    Full Text »    PDF »
Integrin Dynamics and Matrix Assembly: Tensin-dependent Translocation of {alpha}5{beta}1 Integrins Promotes Early Fibronectin Fibrillogenesis.
R. Pankov, E. Cukierman, B.-Z. Katz, K. Matsumoto, D. C. Lin, S. Lin, C. Hahn, and K. M. Yamada (2000)
J. Cell Biol. 148, 1075-1090
   Abstract »    Full Text »    PDF »
Caspase-mediated Cleavage of p130cas in Etoposide-induced Apoptotic Rat-1 Cells.
S. Kook, S. R. Shim, S. J. Choi, J. Ahnn, J. I. Kim, S. H. Eom, Y. K. Jung, S. G. Paik, and W. K. Song (2000)
Mol. Biol. Cell 11, 929-939
   Abstract »    Full Text »
Physical State of the Extracellular Matrix Regulates the Structure and Molecular Composition of Cell-Matrix Adhesions.
B.-Z. Katz, E. Zamir, A. Bershadsky, Z. Kam, K. M. Yamada, and B. Geiger (2000)
Mol. Biol. Cell 11, 1047-1060
   Abstract »    Full Text »
beta 1 Integrins Regulate Keratinocyte Adhesion and Differentiation by Distinct Mechanisms.
L. Levy, S. Broad, D. Diekmann, R. D. Evans, and F. M. Watt (2000)
Mol. Biol. Cell 11, 453-466
   Abstract »    Full Text »
Fibronectin polymerization stimulates cell growth by RGD-dependent and -independent mechanisms.
J Sottile, D. Hocking, and K. Langenbach (2000)
J. Cell Sci. 113, 4287-4299
   Abstract »    PDF »
Actin Activates a Cryptic Dimerization Potential of the Vinculin Tail Domain.
R. P. Johnson and S. W. Craig (2000)
J. Biol. Chem. 275, 95-105
   Abstract »    Full Text »    PDF »
Galectin-8 binding to integrins inhibits cell adhesion and induces apoptosis.
Y. Hadari, R Arbel-Goren, Y Levy, A Amsterdam, R Alon, R Zakut, and Y Zick (2000)
J. Cell Sci. 113, 2385-2397
   Abstract »    PDF »
Collagen degradation and platelet-derived growth factor stimulate the migration of vascular smooth muscle cells.
E Stringa, V Knauper, G Murphy, and J Gavrilovic (2000)
J. Cell Sci. 113, 2055-2064
   Abstract »    PDF »
Cell adhesion and motility depend on nanoscale RGD clustering.
G Maheshwari, G Brown, D. Lauffenburger, A Wells, and L. Griffith (2000)
J. Cell Sci. 113, 1677-1686
   Abstract »    PDF »
Functional interaction between E-cadherin and alphav-containing integrins in carcinoma cells.
M von Schlippe, J. Marshall, P Perry, M Stone, A. Zhu, and I. Hart (2000)
J. Cell Sci. 113, 425-437
   Abstract »    PDF »
Tyrosine Phosphorylation of alpha -Actinin in Activated Platelets.
G. Izaguirre, L. Aguirre, P. Ji, B. Aneskievich, and B. Haimovich (1999)
J. Biol. Chem. 274, 37012-37020
   Abstract »    Full Text »    PDF »
PTEN Gene and Integrin Signaling in Cancer.
M. Tamura, J. Gu, H. Tran, and K. M. Yamada (1999)
J Natl Cancer Inst 91, 1820-1828
   Abstract »    Full Text »    PDF »
Integrin {alpha}2{beta}1 Mediates Isoform-specific Activation of p38 and Upregulation of Collagen Gene Transcription by a Mechanism Involving the {alpha}2 Cytoplasmic Tail.
J. Ivaska, H. Reunanen, J. Westermarck, L. Koivisto, V.-M. Kahari, and J. Heino (1999)
J. Cell Biol. 147, 401-416
   Abstract »    Full Text »    PDF »
Fibronectin Upregulates Gelatinase B (MMP-9) and Induces Coordinated Expression of Gelatinase A (MMP-2) and Its Activator MT1-MMP (MMP-14) by Human T Lymphocyte Cell Lines. A Process Repressed Through RAS/MAP Kinase Signaling Pathways.
J. Esparza, C. Vilardell, J. Calvo, M. Juan, J. Vives, A. Urbano-Marquez, J. Yague, and M. C. Cid (1999)
Blood 94, 2754-2766
   Abstract »    Full Text »    PDF »



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