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Science 10 February 1995: Vol. 267. no. 5199, pp. 883 - 885 DOI: 10.1126/science.7846531
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Articles
Science, Vol 267, Issue 5199, 883-885
Copyright © 1995 by American Association for the Advancement of Science
Synergistic roles for receptor occupancy and aggregation in integrin transmembrane function
S Miyamoto,
SK Akiyama,
and
KM Yamada
Laboratory of Developmental Biology, National Institute of Dental Research, National Institutes of Health, Bethesda, MD 20892-4370.
Integrin receptors mediate cell adhesion, signal transduction, and cytoskeletal organization. How a single transmembrane receptor can fulfill multiple functions was clarified by comparing roles of receptor occupancy and aggregation. Integrin occupancy by monovalent ligand induced receptor redistribution, but minimal tyrosine phosphorylation signaling or cytoskeletal protein redistribution. Aggregation of integrins by noninhibitory monoclonal antibodies on beads induced intracellular accumulations of pp125FAK and tensin, as well as phosphorylation, but no accumulation of other cytoskeletal proteins such as talin. Combining antibody-mediated clustering with monovalent ligand occupancy induced accumulation of seven cytoskeletal proteins, including alpha-actinin, talin, and F-actin, thereby mimicking multivalent interactions with fibronectin or polyvalent peptides. Integrins therefore mediate a complex repertoire of functions through the distinct effects of receptor aggregation, receptor occupancy, or both together.
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J Appl Physiol
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- Differential Dynamics of {alpha}5 Integrin, Paxillin, and {alpha}-Actinin during Formation and Disassembly of Adhesions in Migrating Cells.
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J. Cell Biol.
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- Osteoblast Integrin Adhesion and Signaling Regulate Mineralization.
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Journal of Dental Research
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- Regulation of human lung fibroblast phenotype and function by vitronectin and vitronectin integrins.
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- Endocytosis and Nuclear Trafficking of Adeno-Associated Virus Type 2 Are Controlled by Rac1 and Phosphatidylinositol-3 Kinase Activation.
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- Role of MAP Kinases p42erk-2/p44erk-1 in Cementum-derived Attachment-protein-mediated Cell Attachment.
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Journal of Dental Research
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- Adenovirus E4 Open Reading Frame 4-Induced Apoptosis Involves Dysregulation of Src Family Kinases.
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- Interaction of Extracellular Matrix and Activin-A in the Initiation of Follicle Growth in the Mouse Ovary.
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Biol Reprod
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- Phosphorylation of Tyrosine Residues 31 and 118 on Paxillin Regulates Cell Migration through an Association with Crk in Nbt-II Cells.
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- Integrin Dynamics and Matrix Assembly: Tensin-Dependent Translocation of {alpha}5{beta}1 Integrins Promotes Early Fibronectin Fibrillogenesis.
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- Caspase-mediated Cleavage of p130cas in Etoposide-induced Apoptotic Rat-1 Cells.
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Mol. Biol. Cell
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- Physical State of the Extracellular Matrix Regulates the Structure and Molecular Composition of Cell-Matrix Adhesions.
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Mol. Biol. Cell
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- beta 1 Integrins Regulate Keratinocyte Adhesion and Differentiation by Distinct Mechanisms.
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Mol. Biol. Cell
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- Actin Activates a Cryptic Dimerization Potential of the Vinculin Tail Domain.
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- Galectin-8 binding to integrins inhibits cell adhesion and induces apoptosis.
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- Collagen degradation and platelet-derived growth factor stimulate the migration of vascular smooth muscle cells.
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- Cell adhesion and motility depend on nanoscale RGD clustering.
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- Functional interaction between E-cadherin and alphav-containing integrins in carcinoma cells.
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- Tyrosine Phosphorylation of alpha -Actinin in Activated Platelets.
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