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Science 13 January 1995:
Vol. 267. no. 5195, pp. 234 - 237
DOI: 10.1126/science.7528943
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Articles

Science, Vol 267, Issue 5195, 234-237
Copyright © 1995 by American Association for the Advancement of Science

articles

Identification of bases in 16S rRNA essential for tRNA binding at the 30S ribosomal P site

U von Ahsen and HF Noller

Sinsheimer Laboratories, University of California, Santa Cruz 95064.

Previous studies suggest that the mechanism of action of the ribosome in translation involves crucial transfer RNA (tRNA)-ribosomal RNA (rRNA) interactions. Here, a selection scheme was developed to identify bases in 16S rRNA that are essential for tRNA binding to the P site of the small (30S) ribosomal subunit. Modification of the N-1 and N-2 positions of 2-methylguanine 966 and of the N-7 position of guanine 1401 interfered with messenger RNA (mRNA)-dependent binding of tRNA to the P site. Modification of the same positions as well as of the N-1 and N-2 positions of guanine 926 interfered with mRNA-independent binding of tRNA at high magnesium ion concentration. These results suggest that these three bases are involved in intermolecular contacts between ribosomes and tRNA.


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