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Science 6 January 1995:
Vol. 267. no. 5194, pp. 100 - 104
DOI: 10.1126/science.7809597

Articles

Science, Vol 267, Issue 5194, 100-104
Copyright © 1995 by American Association for the Advancement of Science


articles

p53 transcriptional activation mediated by coactivators TAFII40 and TAFII60

CJ Thut, JL Chen, R Klemm, and R Tjian

Howard Hughes Medical Institute, University of California, Berkeley 94720.

The tumor suppressor protein p53 is a transcriptional regulator that enhances the expression of proteins that control cellular proliferation. The multisubunit transcription factor IID (TFIID) is thought to be a primary target for site-specific activators of transcription. Here, a direct interaction between the activation domain of p53 and two subunits of the TFIID complex, TAFII40 and TAFII60, is reported. A double point mutation in the activation domain of p53 impaired the ability of this domain to activate transcription and, simultaneously, its ability to interact with both TAFII40 and TAFII60. Furthermore, a partial TFIID complex containing Drosophila TATA binding protein (dTBP), human TAFII250, dTAFII60, and dTAFII40 supported activation by a Gal4-p53 fusion protein in vitro, whereas TBP or a subcomplex lacking TAFII40 and TAFII60 did not. Together, these results suggest that TAFII40 and TAFII60 are important targets for transmitting activation signals between p53 and the initiation complex.


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Regulation of the p53 Protein by the MDM2 Oncoprotein--Thirty-eighth G. H. A. Clowes Memorial Award Lecture.
D. A. Freedman (1999)
Cancer Res. 59, 1-7
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Phosphorylation of p53 Serine 15 Increases Interaction with CBP.
P. F. Lambert, F. Kashanchi, M. F. Radonovich, R. Shiekhattar, and J. N. Brady (1998)
J. Biol. Chem. 273, 33048-33053
   Abstract »    Full Text »    PDF »
Nuclear Export Is Required for Degradation of Endogenous p53 by MDM2 and Human Papillomavirus E6.
D. A. Freedman and A. J. Levine (1998)
Mol. Cell. Biol. 18, 7288-7293
   Abstract »    Full Text »
The Oct-1 POU domain activates snRNA gene transcription by contacting a region in the SNAPc largest subunit that bears sequence similarities to the Oct-1 coactivator OBF-1.
E. Ford, M. Strubin, and N. Hernandez (1998)
Genes & Dev. 12, 3528-3540
   Abstract »    Full Text »
Critical Structural Elements and Multitarget Protein Interactions of the Transcriptional Activator AF-1 of Hepatocyte Nuclear Factor 4.
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J. Biol. Chem. 273, 29950-29957
   Abstract »    Full Text »    PDF »
Structure and specificity of nuclear receptor-coactivator interactions.
B. D. Darimont, R. L. Wagner, J. W. Apriletti, M. R. Stallcup, P. J. Kushner, J. D. Baxter, R. J. Fletterick, and K. R. Yamamoto (1998)
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Distinct Subdomains of Human TAFII130 Are Required for Interactions with Glutamine-Rich Transcriptional Activators.
D. Saluja, M. F. Vassallo, and N. Tanese (1998)
Mol. Cell. Biol. 18, 5734-5743
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The complexity of p53 modulation: emerging patterns from divergent signals.
A. J. Giaccia and M. B. Kastan (1998)
Genes & Dev. 12, 2973-2983
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Chromatin, TAFs, and a novel multiprotein coactivator are required for synergistic activation by Sp1 and SREBP-1a in vitro.
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Genes & Dev. 12, 3020-3031
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Phosphorylation of p53: a Novel Pathway for p53 Inactivation in Human T-Cell Lymphotropic Virus Type 1-Transformed Cells.
C. A. Pise-Masison, M. Radonovich, K. Sakaguchi, E. Appella, and J. N. Brady (1998)
J. Virol. 72, 6348-6355
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Activation of c-myc Gene Expression by Tumor-Derived p53 Mutants Requires a Discrete C-Terminal Domain.
M. W. Frazier, X. He, J. Wang, Z. Gu, J. L. Cleveland, and G. P. Zambetti (1998)
Mol. Cell. Biol. 18, 3735-3743
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Suppression of the p300-dependent mdm2 negative-feedback loop induces the p53 apoptotic function.
A. Thomas and E. White (1998)
Genes & Dev. 12, 1975-1985
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Non-p53 p53RE binding protein, a human transcription factor functionally analogous to P53.
X. Zeng, A. J. Levine, and H. Lu (1998)
PNAS 95, 6681-6686
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Transcription Factor IIA Derepresses TATA-binding Protein (TBP)-associated Factor Inhibition of TBP-DNA Binding.
J. Ozer, K. Mitsouras, D. Zerby, M. Carey, and P. M. Lieberman (1998)
J. Biol. Chem. 273, 14293-14300
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Molecular Genetics of the RNA Polymerase II General Transcriptional Machinery.
M. Hampsey (1998)
Microbiol. Mol. Biol. Rev. 62, 465-503
   Abstract »    Full Text »    PDF »
Identification of an additional negative regulatory region for p53 sequence-specific DNA binding.
B. F. Muller-Tiemann, T. D. Halazonetis, and J. J. Elting (1998)
PNAS 95, 6079-6084
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Identification of a Novel p53 Functional Domain That Is Necessary for Mediating Apoptosis.
J. Zhu, W. Zhou, J. Jiang, and X. Chen (1998)
J. Biol. Chem. 273, 13030-13036
   Abstract »    Full Text »    PDF »
Adenovirus Type 12-Induced Fragility of the Human RNU2 Locus Requires p53 Function.
Z. Li, A. Yu, and A. M. Weiner (1998)
J. Virol. 72, 4183-4191
   Abstract »    Full Text »    PDF »
The p53 Tumor Suppressor Inhibits Transcription of the TATA-less Mouse DP1 Promoter.
R. V. Gopalkrishnan, E. W.-F. Lam, and C. Kedinger (1998)
J. Biol. Chem. 273, 10972-10978
   Abstract »    Full Text »    PDF »
Adenovirus E1B 55K Represses p53 Activation In Vitro.
M. E. D. Martin and A. J. Berk (1998)
J. Virol. 72, 3146-3154
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Adf-1 Is a Nonmodular Transcription Factor That Contains a TAF-Binding Myb-Like Motif.
G. Cutler, K. M. Perry, and R. Tjian (1998)
Mol. Cell. Biol. 18, 2252-2261
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The Gcn4p Activation Domain Interacts Specifically In Vitro with RNA Polymerase II Holoenzyme, TFIID, and the Adap-Gcn5p Coactivator Complex.
C. M. Drysdale, B. M. Jackson, R. McVeigh, E. R. Klebanow, Y. Bai, T. Kokubo, M. Swanson, Y. Nakatani, P. A. Weil, and A. G. Hinnebusch (1998)
Mol. Cell. Biol. 18, 1711-1724
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The Yeast TAF145 Inhibitory Domain and TFIIA Competitively Bind to TATA-Binding Protein.
T. Kokubo, M. J. Swanson, J.-i. Nishikawa, A. G. Hinnebusch, and Y. Nakatani (1998)
Mol. Cell. Biol. 18, 1003-1012
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A Hydrophobic Segment within the 81-Amino-Acid Domain of TFIIIA from Saccharomyces cerevisiae Is Essential for Its Transcription Factor Activity.
O. Rowland and J. Segall (1998)
Mol. Cell. Biol. 18, 420-432
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p53--An Acrobat in Tumorigenesis.
U.M. Moll and L.M. Schramm (1998)
Critical Reviews in Oral Biology & Medicine 9, 23-37
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TBP-associated Factors in the PCAF Histone Acetylase Complex.
T. KOTANI, X. ZHANG, R.L. SCHILTZ, V.V. OGRYZKO, T. HOWARD, M.J. SWANSON, A. VASSILEV, H. ZHANG, J. YAMAUCHI, B.H. HOWARD, et al. (1998)
Cold Spring Harb Symp Quant Biol 63, 493-500
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DNA damage induces phosphorylation of the amino terminus of p53.
J. D. Siliciano, C. E. Canman, Y. Taya, K. Sakaguchi, E. Appella, and M. B. Kastan (1997)
Genes & Dev. 11, 3471-3481
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Involvement of the Transcription Factor IID Protein Complex in Gene Activation by the N-Terminal Transactivation Domain of the Glucocorticoid Receptor in Vitro.
J. Ford, I. J. McEwan, A. P. H. Wright, and J.-A. Gustafsson (1997)
Mol. Endocrinol. 11, 1467-1475
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Repression of p53-mediated transcription by MDM2: a dual mechanism.
C. J. Thut, J. A. Goodrich, and R. Tjian (1997)
Genes & Dev. 11, 1974-1986
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Interaction between replication protein A and p53 is disrupted after UV damage in a DNA repair-dependent manner.
N. A. Abramova, J. Russell, M. Botchan, and R. Li (1997)
PNAS 94, 7186-7191
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Identification of a novel p53 functional domain that is necessary for efficient growth suppression.
K. K. Walker and A. J. Levine (1996)
PNAS 93, 15335-15340
   Abstract »    Full Text »    PDF »
SREBP transcriptional activity is mediated through an interaction with the CREB-binding protein..
J D Oliner, J M Andresen, S K Hansen, S Zhou, and R Tjian (1996)
Genes & Dev. 10, 2903-2911
   Abstract »    PDF »
Mitotic regulation of TFIID: inhibition of activator-dependent transcription and changes in subcellular localization..
N Segil, M Guermah, A Hoffmann, R G Roeder, and N Heintz (1996)
Genes & Dev. 10, 2389-2400
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p53 levels, functional domains, and DNA damage determine the extent of the apoptotic response of tumor cells..
X Chen, L J Ko, L Jayaraman, and C Prives (1996)
Genes & Dev. 10, 2438-2451
   Abstract »    PDF »
Yeast TAF(II)90 is required for cell-cycle progression through G2/M but not for general transcription activation..
L M Apone, C M Virbasius, J C Reese, and M R Green (1996)
Genes & Dev. 10, 2368-2380
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Multiple Interactions between hTAFII55 and Other TFIID Subunits. REQUIREMENTS FOR THE FORMATION OF STABLE TERNARY COMPLEXES BETWEEN hTAFII55 AND THE TATA-BINDING PROTEIN.
A.-C. Lavigne, G. Mengus, M. May, V. Dubrovskaya, L. Tora, P. Chambon, and I. Davidson (1996)
J. Biol. Chem. 271, 19774-19780
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Cloning and Characterization of Human TAF20/15. MULTIPLE INTERACTIONS SUGGEST A CENTRAL ROLE IN TFIID COMPLEX FORMATION.
A. Hoffmann and R. G. Roeder (1996)
J. Biol. Chem. 271, 18194-18202
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p53 Is a Mediator for Radiation-repressed Human TR2 Orphan Receptor Expression in MCF-7 Cells, a New Pathway from Tumor Suppressor to Member of the Steroid Receptor Superfamily.
D.-L. Lin and C. Chang (1996)
J. Biol. Chem. 271, 14649-14652
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p53 Stimulates Promoter Activity of the sgk Serum/Glucocorticoid-inducible Serine/Threonine Protein Kinase Gene in Rodent Mammary Epithelial Cells.
A. C. Maiyar, A. J. Huang, P. T. Phu, H. H. Cha, and G. L. Firestone (1996)
J. Biol. Chem. 271, 12414-12422
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The XPB and XPD DNA helicases are components of the p53-mediated apoptosis pathway..
X W Wang, W Vermeulen, J D Coursen, M Gibson, S E Lupold, K Forrester, G Xu, L Elmore, H Yeh, J H Hoeijmakers, et al. (1996)
Genes & Dev. 10, 1219-1232
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Transcription Factor IIA Mutations Show Activator-specific Defects and Reveal a IIA Function Distinct from Stimulation of TBP-DNA Binding.
J. Ozer, A. H. Bolden, and P. M. Lieberman (1996)
J. Biol. Chem. 271, 11182-11190
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p53: puzzle and paradigm..
L J Ko and C Prives (1996)
Genes & Dev. 10, 1054-1072
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Wild-type p53 transgenic mice exhibit altered differentiation of the ureteric bud and possess small kidneys..
L A Godley, J B Kopp, M Eckhaus, J J Paglino, J Owens, and H E Varmus (1996)
Genes & Dev. 10, 836-850
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Allosteric Regulation of the Thermostability and DNA Binding Activity of Human p53 by Specific Interacting Proteins.
S. Hansen, T. R. Hupp, and D. P. Lane (1996)
J. Biol. Chem. 271, 3917-3924
   Abstract »    Full Text »    PDF »
DNA Template and Activator-Coactivator Requirements for Transcriptional Synergism by Drosophila Bicoid.
F. Sauer, S. K. Hansen, and R. Tjian (1995)
Science 270, 1825-1828
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Coactivator and Promoter-Selective Properties of RNA Polymerase I TAFs.
H. Beckmann, J.-L. Chen, T. O'Brien, and R. Tjian (1995)
Science 270, 1506-1509
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Human TAFII250 interacts with RAP74: implications for RNA polymerase II initiation..
S Ruppert and R Tjian (1995)
Genes & Dev. 9, 2747-2755
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Transforming Growth Factor beta Activates the Promoter of Cyclin-dependent Kinase Inhibitor p15[IMAGE]through an Sp1 Consensus Site.
J.-M. Li, M. A. Nichols, S. Chandrasekharan, Y. Xiong, and X.-F. Wang (1995)
J. Biol. Chem. 270, 26750-26753
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Upstream Enhancer Activity in the Human Surfactant Protein B Gene Is Mediated by Thyroid Transcription Factor 1.
C. Yan, Z. Sever, and J. A. Whitsett (1995)
J. Biol. Chem. 270, 24852-24857
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Transactivation Ability of p53 Transcriptional Activation Domain Is Directly Related to the Binding Affinity to TATA-binding Protein.
J. Chang, D.-H. Kim, S. W. Lee, K. Y. Choi, and Y. C. Sung (1995)
J. Biol. Chem. 270, 25014-25019
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Identification of a Transactivation Function in the Progesterone Receptor That Interacts with the TAF[IMAGE]110 Subunit of the TFIID Complex.
C. Schwerk, M. Klotzbücher, M. Sachs, V. Ulber, and L. Klein-Hitpass (1995)
J. Biol. Chem. 270, 21331-21338
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Induction of apoptosis in HeLa cells by trans-activation-deficient p53..
Y Haupt, S Rowan, E Shaulian, K H Vousden, and M Oren (1995)
Genes & Dev. 9, 2170-2183
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