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Science 6 January 1995: Vol. 267. no. 5194, pp. 100 - 104 DOI: 10.1126/science.7809597
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Articles
Science, Vol 267, Issue 5194, 100-104
Copyright © 1995 by American Association for the Advancement of Science
p53 transcriptional activation mediated by coactivators TAFII40 and TAFII60
CJ Thut,
JL Chen,
R Klemm,
and
R Tjian
Howard Hughes Medical Institute, University of California, Berkeley 94720.
The tumor suppressor protein p53 is a transcriptional regulator that enhances the expression of proteins that control cellular proliferation. The multisubunit transcription factor IID (TFIID) is thought to be a primary target for site-specific activators of transcription. Here, a direct interaction between the activation domain of p53 and two subunits of the TFIID complex, TAFII40 and TAFII60, is reported. A double point mutation in the activation domain of p53 impaired the ability of this domain to activate transcription and, simultaneously, its ability to interact with both TAFII40 and TAFII60. Furthermore, a partial TFIID complex containing Drosophila TATA binding protein (dTBP), human TAFII250, dTAFII60, and dTAFII40 supported activation by a Gal4-p53 fusion protein in vitro, whereas TBP or a subcomplex lacking TAFII40 and TAFII60 did not. Together, these results suggest that TAFII40 and TAFII60 are important targets for transmitting activation signals between p53 and the initiation complex.
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- p53 Stimulates Promoter Activity of the sgk Serum/Glucocorticoid-inducible Serine/Threonine Protein Kinase Gene in Rodent Mammary Epithelial Cells.
- A. C. Maiyar, A. J. Huang, P. T. Phu, H. H. Cha, and G. L. Firestone (1996)
J. Biol. Chem.
271, 12414-12422
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- The XPB and XPD DNA helicases are components of the p53-mediated apoptosis pathway..
- X W Wang, W Vermeulen, J D Coursen, M Gibson, S E Lupold, K Forrester, G Xu, L Elmore, H Yeh, J H Hoeijmakers, et al. (1996)
Genes & Dev.
10, 1219-1232
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- Transcription Factor IIA Mutations Show Activator-specific Defects and Reveal a IIA Function Distinct from Stimulation of TBP-DNA Binding.
- J. Ozer, A. H. Bolden, and P. M. Lieberman (1996)
J. Biol. Chem.
271, 11182-11190
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- p53: puzzle and paradigm..
- L J Ko and C Prives (1996)
Genes & Dev.
10, 1054-1072
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- Wild-type p53 transgenic mice exhibit altered differentiation of the ureteric bud and possess small kidneys..
- L A Godley, J B Kopp, M Eckhaus, J J Paglino, J Owens, and H E Varmus (1996)
Genes & Dev.
10, 836-850
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- Allosteric Regulation of the Thermostability and DNA Binding Activity of Human p53 by Specific Interacting Proteins.
- S. Hansen, T. R. Hupp, and D. P. Lane (1996)
J. Biol. Chem.
271, 3917-3924
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- DNA Template and Activator-Coactivator Requirements for Transcriptional Synergism by Drosophila Bicoid.
- F. Sauer, S. K. Hansen, and R. Tjian (1995)
Science
270, 1825-1828
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- Coactivator and Promoter-Selective Properties of RNA Polymerase I TAFs.
- H. Beckmann, J.-L. Chen, T. O'Brien, and R. Tjian (1995)
Science
270, 1506-1509
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- Human TAFII250 interacts with RAP74: implications for RNA polymerase II initiation..
- S Ruppert and R Tjian (1995)
Genes & Dev.
9, 2747-2755
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- Transforming Growth Factor beta Activates the Promoter of Cyclin-dependent Kinase Inhibitor p15[IMAGE]through an Sp1 Consensus Site.
- J.-M. Li, M. A. Nichols, S. Chandrasekharan, Y. Xiong, and X.-F. Wang (1995)
J. Biol. Chem.
270, 26750-26753
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- Upstream Enhancer Activity in the Human Surfactant Protein B Gene Is Mediated by Thyroid Transcription Factor 1.
- C. Yan, Z. Sever, and J. A. Whitsett (1995)
J. Biol. Chem.
270, 24852-24857
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- Transactivation Ability of p53 Transcriptional Activation Domain Is Directly Related to the Binding Affinity to TATA-binding Protein.
- J. Chang, D.-H. Kim, S. W. Lee, K. Y. Choi, and Y. C. Sung (1995)
J. Biol. Chem.
270, 25014-25019
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- Identification of a Transactivation Function in the Progesterone Receptor That Interacts with the TAF[IMAGE]110 Subunit of the TFIID Complex.
- C. Schwerk, M. Klotzbücher, M. Sachs, V. Ulber, and L. Klein-Hitpass (1995)
J. Biol. Chem.
270, 21331-21338
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- Induction of apoptosis in HeLa cells by trans-activation-deficient p53..
- Y Haupt, S Rowan, E Shaulian, K H Vousden, and M Oren (1995)
Genes & Dev.
9, 2170-2183
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