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Science 16 December 1994:
Vol. 266. no. 5192, pp. 1878 - 1881
DOI: 10.1126/science.7997882
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Articles

Science, Vol 266, Issue 5192, 1878-1881
Copyright © 1994 by American Association for the Advancement of Science

articles

Specification of C/EBP function during Drosophila development by the bZIP basic region

P Rorth

Department of Embryology, Carnegie Institution of Washington, Baltimore, MD 21210.

The biologically relevant interactions of a transcription factor are those that are important for function in the organism. Here, a transgenic rescue assay was used to determine which molecular functions of Drosophila CCAAT/enhancer binding protein (C/EBP), a basic region-leucine zipper transcription factor, are required for it to fulfill its essential role during development. Chimeric proteins that contain the Drosophila C/EBP (DmC/EBP) basic region, a heterologous zipper, and a heterologous activation domain could functionally substitute for DmC/EBP. Mammalian C/EBPs were also functional in Drosophila. In contrast, 9 of 25 single amino acid substitutions in the basic region disrupted biological function. Thus, the conserved basic region specifies DmC/EBP activity in the organism.


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