Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 16 December 1994:
Vol. 266. no. 5192, pp. 1870 - 1874
DOI: 10.1126/science.7997880
Prev | Table of Contents | Next

Articles

Science, Vol 266, Issue 5192, 1870-1874
Copyright © 1994 by American Association for the Advancement of Science

articles

Toxic shock syndrome toxin-1 complexed with a class II major histocompatibility molecule HLA-DR1

J Kim, RG Urban, JL Strominger, and DC Wiley

Howard Hughes Medical Institute, Children's Hospital, Boston, MA 02115.

The three-dimensional structure of a Staphylococcus aureus superantigen, toxic shock syndrome toxin-1 (TSST-1), complexed with a human class II major histocompatibility molecule (DR1), was determined by x-ray crystallography. The TSST-1 binding site on DR1 overlaps that of the superantigen S. aureus enterotoxin B (SEB), but the two binding modes differ. Whereas SEB binds primarily off one edge of the peptide binding site of DR1, TSST-1 extends over almost one-half of the binding site and contacts both the flanking alpha helices of the histocompatibility antigen and the bound peptide. This difference suggests that the T cell receptor (TCR) would bind to TSST-1:DR1 very differently than to DR1:peptide or SEB:DR1. It also suggests that TSST-1 binding may be dependent on the peptide, though less so than TCR binding, providing a possible explanation for the inability of TSST-1 to competitively block SEB binding to all DR1 molecules on cells (even though the binding sites of TSST-1 and SEB on DR1 overlap almost completely) and suggesting the possibility that T cell activation by superantigen could be directed by peptide antigen.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Molecular Requirements for MHC Class II {alpha}-Chain Engagement and Allelic Discrimination by the Bacterial Superantigen Streptococcal Pyrogenic Exotoxin C.
K. J. Kasper, W. Xi, A. K. M. Nur-ur Rahman, M. M. Nooh, M. Kotb, E. J. Sundberg, J. Madrenas, and J. K. McCormick (2008)
J. Immunol. 181, 3384-3392
   Abstract »    Full Text »    PDF »
Zinc Induces Dimerization of the Class II Major Histocompatibility Complex Molecule That Leads to Cooperative Binding to a Superantigen.
H. Li, Y. Zhao, Y. Guo, Z. Li, L. Eisele, and W. Mourad (2007)
J. Biol. Chem. 282, 5991-6000
   Abstract »    Full Text »    PDF »
Molecular Basis of TCR Selectivity, Cross-Reactivity, and Allelic Discrimination by a Bacterial Superantigen: Integrative Functional and Energetic Mapping of the SpeC-Vbeta2.1 Molecular Interface.
A. K. M. N.-u. Rahman, C. A. Herfst, B. Moza, S. R. Shames, L. A. Chau, C. Bueno, J. Madrenas, E. J. Sundberg, and J. K. McCormick (2006)
J. Immunol. 177, 8595-8603
   Abstract »    Full Text »    PDF »
The TCR V{beta} signature of bacterial superantigens spreads with stimulus strength.
M. Llewelyn, S. Sriskandan, N. Terrazzini, J. Cohen, and D. M. Altmann (2006)
Int. Immunol. 18, 1433-1441
   Abstract »    Full Text »    PDF »
Crystal Structure of Staphylococcal Enterotoxin I (SEI) in Complex with a Human Major Histocompatibility Complex Class II Molecule.
M. M. Fernandez, R. Guan, C. P. Swaminathan, E. L. Malchiodi, and R. A. Mariuzza (2006)
J. Biol. Chem. 281, 25356-25364
   Abstract »    Full Text »    PDF »
Persistence of Zinc-Binding Bacterial Superantigens at the Surface of Antigen-Presenting Cells Contributes to the Extreme Potency of These Superantigens as T-Cell Activators.
D. D. Pless, G. Ruthel, E. K. Reinke, R. G. Ulrich, and S. Bavari (2005)
Infect. Immun. 73, 5358-5366
   Abstract »    Full Text »    PDF »
A Defective Viral Superantigen-Presenting Phenotype in HLA-DR Transfectants Is Corrected by CIITA.
G. A. Azar, R.-P. Sekaly, and J. Thibodeau (2005)
J. Immunol. 174, 7548-7557
   Abstract »    Full Text »    PDF »
Crystallographic and Mutational Data Show That the Streptococcal Pyrogenic Exotoxin J Can Use a Common Binding Surface for T-cell Receptor Binding and Dimerization.
H. M. Baker, T. Proft, P. D. Webb, V. L. Arcus, J. D. Fraser, and E. N. Baker (2004)
J. Biol. Chem. 279, 38571-38576
   Abstract »    Full Text »    PDF »
Structural Relationships and Cellular Tropism of Staphylococcal Superantigen-Like Proteins.
A. M. Al-Shangiti, C. E. Naylor, S. P. Nair, D. C. Briggs, B. Henderson, and B. M. Chain (2004)
Infect. Immun. 72, 4261-4270
   Abstract »    Full Text »    PDF »
Design of Chimeric Receptor Mimics with Different TcRV{beta} Isoforms: TYPE-SPECIFIC INHIBITION OF SUPERANTIGEN PATHOGENESIS.
E. Hong-Geller, M. Mollhoff, P. R. Shiflett, and G. Gupta (2004)
J. Biol. Chem. 279, 5676-5684
   Abstract »    Full Text »    PDF »
Functional Analysis of the TCR Binding Domain of Toxic Shock Syndrome Toxin-1 Predicts Further Diversity in MHC Class II/Superantigen/TCR Ternary Complexes.
J. K. McCormick, T. J. Tripp, A. S. Llera, E. J. Sundberg, M. M. Dinges, R. A. Mariuzza, and P. M. Schlievert (2003)
J. Immunol. 171, 1385-1392
   Abstract »    Full Text »    PDF »
Zinc Binding and Dimerization of Streptococcus pyogenes Pyrogenic Exotoxin C Are Not Essential for T-cell Stimulation.
W. Swietnicki, A. M. Barnie, B. K. Dyas, and R. G. Ulrich (2003)
J. Biol. Chem. 278, 9885-9895
   Abstract »    Full Text »    PDF »
The Zinc-Dependent Major Histocompatibility Complex Class II Binding Site of Streptococcal Pyrogenic Exotoxin C Is Critical for Maximal Superantigen Function and Toxic Activity.
T. J. Tripp, J. K. McCormick, J. M. Webb, and P. M. Schlievert (2003)
Infect. Immun. 71, 1548-1550
   Abstract »    Full Text »    PDF »
The Three-dimensional Structure of a Superantigen-like Protein, SET3, from a Pathogenicity Island of the Staphylococcus aureus Genome.
V. L. Arcus, R. Langley, T. Proft, J. D. Fraser, and E. N. Baker (2002)
J. Biol. Chem. 277, 32274-32281
   Abstract »    Full Text »    PDF »
Identification of MHC Class II-Associated Peptides That Promote the Presentation of Toxic Shock Syndrome Toxin-1 to T Cells.
R. J. Hogan, J. VanBeek, D. R. Broussard, S. L. Surman, and D. L. Woodland (2001)
J. Immunol. 166, 6514-6522
   Abstract »    Full Text »    PDF »
Relaxed DM Requirements During Class II Peptide Loading and CD4+ T Cell Maturation in BALB/c Mice.
E. K. Bikoff, G. Wutz, G. A. Kenty, C. H. Koonce, and E. J. Robertson (2001)
J. Immunol. 166, 5087-5098
   Abstract »    Full Text »    PDF »
Biochemical and Biological Properties of Staphylococcal Enterotoxin K.
P. M. Orwin, D. Y. M. Leung, H. L. Donahue, R. P. Novick, and P. M. Schlievert (2001)
Infect. Immun. 69, 360-366
   Abstract »    Full Text »    PDF »
Toxoids of Streptococcal Pyrogenic Exotoxin A Are Protective in Rabbit Models of Streptococcal Toxic Shock Syndrome.
M. Roggiani, J. A. Stoehr, S. B. Olmsted, Y. V. Matsuka, S. Pillai, D. H. Ohlendorf, and P. M. Schlievert (2000)
Infect. Immun. 68, 5011-5017
   Abstract »    Full Text »    PDF »
Development of Streptococcal Pyrogenic Exotoxin C Vaccine Toxoids That Are Protective in the Rabbit Model of Toxic Shock Syndrome.
J. K. McCormick, T. J. Tripp, S. B. Olmsted, Y. V. Matsuka, P. J. Gahr, D. H. Ohlendorf, and P. M. Schlievert (2000)
J. Immunol. 165, 2306-2312
   Abstract »    Full Text »    PDF »
Inhibition of Staphylococcal Enterotoxin B-Induced Lymphocyte Proliferation and Tumor Necrosis Factor Alpha Secretion by MAb5, an Anti-Toxic Shock Syndrome Toxin 1 Monoclonal Antibody.
L. T. Y. Pang, W. W. S. Kum, and A. W. Chow (2000)
Infect. Immun. 68, 3261-3268
   Abstract »    Full Text »    PDF »
Zinc-Altered Immune Function and Cytokine Production.
L. Rink and H. Kirchner (2000)
J. Nutr. 130, 1407S-1411
   Abstract »    Full Text »
Effect of Molecular Size on the Ability of Zwitterionic Polysaccharides to Stimulate Cellular Immunity.
W. M. Kalka-Moll, A. O. Tzianabos, Y. Wang, V. J. Carey, R. W. Finberg, A. B. Onderdonk, and D. L. Kasper (2000)
J. Immunol. 164, 719-724
   Abstract »    Full Text »    PDF »
Exotoxins of Staphylococcus aureus.
M. M. Dinges, P. M. Orwin, and P. M. Schlievert (2000)
Clin. Microbiol. Rev. 13, 16-34
   Abstract »    Full Text »    PDF »
Staphylococcal Enterotoxin H Displays Unique MHC Class II-Binding Properties.
H. Nilsson, P. Bjork, M. Dohlsten, and P. Antonsson (1999)
J. Immunol. 163, 6686-6693
   Abstract »    Full Text »    PDF »
Binding Motifs of Copolymer 1 to Multiple Sclerosis- and Rheumatoid Arthritis-Associated HLA-DR Molecules.
M. Fridkis-Hareli, J. M. Neveu, R. A. Robinson, W. S. Lane, L. Gauthier, K. W. Wucherpfennig, M. Sela, and J. L. Strominger (1999)
J. Immunol. 162, 4697-4704
   Abstract »    Full Text »    PDF »
Mapping of Staphylococcal Enterotoxin A Functional Binding Sites and Presentation by Monoclonal Antibodies and Fusion Proteins.
W. Mahana (1999)
Infect. Immun. 67, 1894-1900
   Abstract »    Full Text »    PDF »
Identification and Characterization of Novel Superantigens from Streptococcus pyogenes.
T. Proft, S. Louise Moffatt, C. J. Berkahn, and J. D. Fraser (1999)
J. Exp. Med. 189, 89-102
   Abstract »    Full Text »    PDF »
Antitumor Response Elicited by a Superantigen- Transmembrane Sequence Fusion Protein Anchored onto Tumor Cells.
J. L. Wahlsten, C. D. Mills, and S. Ramakrishnan (1998)
J. Immunol. 161, 6761-6767
   Abstract »    Full Text »    PDF »
Mutational Analysis of Superantigen Activity Responsible for the Induction of Skin Erythema by Streptococcal Pyrogenic Exotoxin C.
J. Yamaoka, E. Nakamura, Y. Takeda, S. Imamura, and N. Minato (1998)
Infect. Immun. 66, 5020-5026
   Abstract »    Full Text »    PDF »
The Assembly and Stability of MHC Class II-({alpha}{beta})2 Superdimers.
P. H. Schafer, S. Malapati, K. K. Hanfelt, and S. K. Pierce (1998)
J. Immunol. 161, 2307-2316
   Abstract »    Full Text »    PDF »
Promiscuous Binding of Synthetic Copolymer 1 to Purified HLA-DR Molecules.
M. Fridkis-Hareli and J. L. Strominger (1998)
J. Immunol. 160, 4386-4397
   Abstract »    Full Text »    PDF »
Mtv-1 Superantigen Trafficks Independently of Major Histocompatibility Complex Class II Directly to the B-Cell Surface by the Exocytic Pathway.
M. E. Grigg, C. W. McMahon, S. Morkowski, A. Y. Rudensky, and A. M. Pullen (1998)
J. Virol. 72, 2577-2588
   Abstract »    Full Text »    PDF »
Activation of Bovine Lymphocyte Subpopulations by Staphylococcal Enterotoxin C.
W. A. Ferens, W. C. Davis, M. J. Hamilton, Y. H. Park, C. F. Deobald, L. Fox, and G. Bohach (1998)
Infect. Immun. 66, 573-580
   Abstract »    Full Text »    PDF »
Separation of Function Between the Domains of Toxic Shock Syndrome Toxin-1.
J. L. Wahlsten and S. Ramakrishnan (1998)
J. Immunol. 160, 854-859
   Abstract »    Full Text »    PDF »
Staphylococcal Enterotoxin D Is a Promiscuous Superantigen Offering Multiple Modes of Interactions With the MHC Class II Receptors.
R. Al-Daccak, K. Mehindate, F. Damdoumi, P. Etongue-Mayer, H. Nilsson, P. Antonsson, M. Sundstrom, M. Dohlsten, R.-P. Sekaly, and W. Mourad (1998)
J. Immunol. 160, 225-232
   Abstract »    Full Text »    PDF »
The Superantigen Streptococcal Pyrogenic Exotoxin C (SPE-C) Exhibits a Novel Mode of Action.
P.-L. Li, R. E. Tiedemann, S. L. Moffat, and J. D. Fraser (1997)
J. Exp. Med. 186, 375-383
   Abstract »    Full Text »    PDF »
Selective binding of bacterial toxins to major histocompatibility complex class II-expressing cells is controlled by invariant chain and HLA-DM.
P. M. Lavoie, J. Thibodeau, I. Cloutier, R. Busch, and R.-P. Sekaly (1997)
PNAS 94, 6892-6897
   Abstract »    Full Text »    PDF »
Self and viral peptides can initiate lysis by autologous natural killer cells.
O. Mandelboim, S. B. Wilson, M. Vales-Gomez, H. T. Reyburn, and J. L. Strominger (1997)
PNAS 94, 4604-4609
   Abstract »    Full Text »    PDF »
Paracrine Transfer of Mouse Mammary Tumor Virus Superantigen.
M. Delcourt, J. Thibodeau, F. Denis, and R.-P. Sekaly (1997)
J. Exp. Med. 185, 471-480
   Abstract »    Full Text »    PDF »
The Co-crystal Structure of Staphylococcal Enterotoxin Type A With Zn2+ at 2.7AResolution. IMPLICATIONS FOR MAJOR HISTOCOMPATIBILITY COMPLEX CLASS II BINDING.
M. Sundstrom, D. Hallen, A. Svensson, E. Schad, M. Dohlsten, and L. Abrahmsen (1996)
J. Biol. Chem. 271, 32212-32216
   Abstract »    Full Text »    PDF »
Staphylococcus aureus Expresses a Major Histocompatibility Complex Class II Analog.
K. Jönsson, D. McDevitt, M. H. McGavin, J. M. Patti, and M. Höök (1995)
J. Biol. Chem. 270, 21457-21460
   Abstract »    Full Text »    PDF »
Superantigen-dependent, cell-mediated cytotoxicity inhibited by MHC class I receptors on T lymphocytes.
J. Phillips, J. Gumperz, P Parham, and L. Lanier (1995)
Science 268, 403-405
   Abstract »    PDF »
Subsets of HLA-DR1 molecules defined by SEB and TSST-1 binding.
J Thibodeau, I Cloutier, P. Lavoie, N Labrecque, W Mourad, T Jardetzky, and R. Sekaly (1994)
Science 266, 1874-1878
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)