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Science 9 December 1994:
Vol. 266. no. 5191, pp. 1685 - 1688
DOI: 10.1126/science.7527587
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Articles

Science, Vol 266, Issue 5191, 1685-1688
Copyright © 1994 by American Association for the Advancement of Science

articles

The stereochemical course of group II intron self-splicing

RA Padgett, M Podar, SC Boulanger, and PS Perlman

Department of Biochemistry, University of Texas Southwestern Medical Center at Dallas 75235.

The stereochemical specificities and reaction courses for both self-splicing steps of a group II intron have been determined by phosphorothioate substitution at the 5' and 3' splice site phosphodiester bonds. Both steps of the splicing reaction proceeded with a phosphorothioate in the Sp configuration but were blocked by the Rp diastereomer. Both steps also proceeded with inversion of stereochemical configuration around phosphorus, consistent with a concerted transesterification reaction. These results are identical to those found for nuclear precursor mRNA (pre-mRNA) splicing and provide support for the hypothesis that group II introns and nuclear pre-mRNA introns share a common evolutionary history.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
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SURVEY AND SUMMARY: Recent advances in the elucidation of the mechanisms of action of ribozymes.
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Metal ion catalysis during group II intron self-splicing: parallels with the spliceosome.
E. J. Sontheimer, P. M. Gordon, and J. A. Piccirilli (1999)
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