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Science 2 December 1994:
Vol. 266. no. 5190, pp. 1558 - 1561
DOI: 10.1126/science.7985025
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Articles

Science, Vol 266, Issue 5190, 1558-1561
Copyright © 1994 by American Association for the Advancement of Science

articles

Requirement for intron-encoded U22 small nucleolar RNA in 18S ribosomal RNA maturation

KT Tycowski, MD Shu, and JA Steitz

Howard Hughes Medical Institute, Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, CT 06536.

The nucleoli of vertebrate cells contain a number of small RNAs that are generated by the processing of intron fragments of protein-coding gene transcripts. The host gene (UHG) for intro-encoded human U22 is unusual in that it specifies a polyadenylated but apparently noncoding RNA. Depletion of U22 from Xenopus oocytes by oligonucleotide-directed ribonuclease H targeting prevented the processing of 18S ribosomal RNA (rRNA) at both ends. The appearance of 18S rRNA was restored by injection of in vitro-synthesized U22 RNA. These results identify a cellular function for an intron-encoded small RNA.


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