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Science 25 November 1994:
Vol. 266. no. 5189, pp. 1403 - 1405
DOI: 10.1126/science.7973733
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Articles

Science, Vol 266, Issue 5189, 1403-1405
Copyright © 1994 by American Association for the Advancement of Science

articles

Binding of mismatched microsatellite DNA sequences by the human MSH2 protein

R Fishel, A Ewel, S Lee, MK Lescoe, and J Griffith

Department of Microbiology and Molecular Genetics, Markey Center for Molecular Genetics, University of Vermont School of Medicine, Burlington 05405.

Alteration of the human mismatch repair gene hMSH2 has been linked to the microsatellite DNA instability found in hereditary nonpolyposis colon cancer and several sporadic cancers. This microsatellite DNA instability is thought to arise from defective repair of DNA replication errors that create insertion-deletion loop-type (IDL) mismatched nucleotides. Here, it is shown that purified hMSH2 protein efficiently and specifically binds DNA containing IDL mismatches of up to 14 nucleotides. These results support a direct role for hMSH2 in mutation avoidance and microsatellite stability in human cells.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Prognostic and Predictive Roles of High-Degree Microsatellite Instability in Colon Cancer: A National Cancer Institute-National Surgical Adjuvant Breast and Bowel Project Collaborative Study.
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J. Clin. Oncol. 25, 767-772
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Analysis of the Interaction between the Saccharomyces cerevisiae MSH2-MSH6 and MLH1-PMS1 Complexes with DNA Using a Reversible DNA End-blocking System.
M. L. Mendillo, D. J. Mazur, and R. D. Kolodner (2005)
J. Biol. Chem. 280, 22245-22257
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The Hereditary Nonpolyposis Colorectal Cancer Syndrome: Genetics and Clinical Implications.
D. C. Chung and A. K. Rustgi (2003)
Ann Intern Med 138, 560-570
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Genetic Counseling and Testing in Families With Hereditary Nonpolyposis Colorectal Cancer.
D. W. Hadley, J. Jenkins, E. Dimond, K. Nakahara, L. Grogan, D. J. Liewehr, S. M. Steinberg, and I. Kirsch (2003)
Arch Intern Med 163, 573-582
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Dynamics of beta and Proliferating Cell Nuclear Antigen Sliding Clamps in Traversing DNA Secondary Structure.
N. Yao, J. Hurwitz, and M. O'Donnell (2000)
J. Biol. Chem. 275, 1421-1432
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The RAD2 Domain of Human Exonuclease 1 Exhibits 5' to 3' Exonuclease and Flap Structure-specific Endonuclease Activities.
B.-I. Lee and D. M. Wilson III (1999)
J. Biol. Chem. 274, 37763-37769
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Separation-of-Function Mutations in Saccharomyces cerevisiae MSH2 That Confer Mismatch Repair Defects but Do Not Affect Nonhomologous-Tail Removal during Recombination.
B. Studamire, G. Price, N. Sugawara, J. E. Haber, and E. Alani (1999)
Mol. Cell. Biol. 19, 7558-7567
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Biochemical Characterization of the Interaction between the Saccharomyces cerevisiae MSH2-MSH6 Complex and Mispaired Bases in DNA.
G. T. Marsischky and R. D. Kolodner (1999)
J. Biol. Chem. 274, 26668-26682
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Dissociation of Mismatch Recognition and ATPase Activity by hMSH2-hMSH3.
T. Wilson, S. Guerrette, and R. Fishel (1999)
J. Biol. Chem. 274, 21659-21664
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Microsatellite Instability and p53 Mutations Are Associated With Abnormal Expression of the MSH2 Gene in Adult Acute Leukemia.
Y.-M. Zhu, E.P. Das-Gupta, and N.H. Russell (1999)
Blood 94, 733-740
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`Saccharomyces cerevisiae MSH2/6 Complex Interacts with Holliday Junctions and Facilitates Their Cleavage by Phage Resolution Enzymes.
G. T. Marsischky, S. Lee, J. Griffith, and R. D. Kolodner (1999)
J. Biol. Chem. 274, 7200-7206
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hMSH5: A Human MutS Homologue That Forms a Novel Heterodimer with hMSH4 and Is Expressed during Spermatogenesis.
T. Bocker, A. Barusevicius, T. Snowden, D. Rasio, S. Guerrette, D. Robbins, C. Schmidt, J. Burczak, C. M. Croce, T. Copeland, et al. (1999)
Cancer Res. 59, 816-822
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Isolation of MutSbeta from Human Cells and Comparison of the Mismatch Repair Specificities of MutSbeta and MutSalpha.
J. Genschel, S. J. Littman, J. T. Drummond, and P. Modrich (1998)
J. Biol. Chem. 273, 19895-19901
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Mismatch repair, molecular switches, and signal transduction.
R. Fishel (1998)
Genes & Dev. 12, 2096-2101
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Prediction-based threading of the hMSH2 DNA mismatch repair protein.
M. De las Alas, R. A. M. de bruin, L. T. Eyck, G. Los, and S. B. Howell (1998)
FASEB J 12, 653-663
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Functional Overlap in Mismatch Repair by Human MSH3 and MSH6.
A. Umar, J. I. Risinger, W. E. Glaab, K. R. Tindall, J. C. Barrett, and T. A. Kunkel (1998)
Genetics 148, 1637-1646
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Recombination-dependent deletion formation in mammalian cells deficient in the nucleotide excision repair gene ERCC1.
R. G. Sargent, R. L. Rolig, A. E. Kilburn, G. M. Adair, J. H. Wilson, and R. S. Nairn (1997)
PNAS 94, 13122-13127
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Extended Abstracts.
(1997)
Toxicol Pathol 25, 674-696
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Methyl-directed Repair of Mismatched Small Heterologous Sequences in Cell Extracts from Escherichia coli.
W.-h. Fang, J.-Y. Wu, and M.-J. Su (1997)
J. Biol. Chem. 272, 22714-22720
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Cleavage of Insertion/Deletion Mismatches, Flap and Pseudo-Y DNA Structures by Deoxyinosine 3'-Endonuclease from Escherichia coli.
M. Yao and Y. W. Kow (1996)
J. Biol. Chem. 271, 30672-30676
   Abstract »    Full Text »    PDF »
hMSH2 forms specific mispair-binding complexes with hMSH3 and hMSH6.
S. Acharya, T. Wilson, S. Gradia, M. F. Kane, S. Guerrette, G. T. Marsischky, R. Kolodner, and R. Fishel (1996)
PNAS 93, 13629-13634
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Human MutSalpha Specifically Binds to DNA Containing Aminofluorene and Acetylaminofluorene Adducts.
G.-M. Li, H. Wang, and L. J. Romano (1996)
J. Biol. Chem. 271, 24084-24088
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Biochemistry and genetics of eukaryotic mismatch repair..
R Kolodner (1996)
Genes & Dev. 10, 1433-1442
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Requirement of the Yeast MSH3 and MSH6 Genes for MSH2-dependent Genomic Stability.
R. E. Johnson, G. K. Kovvali, L. Prakash, and S. Prakash (1996)
J. Biol. Chem. 271, 7285-7288
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Identification and Characterization of a Thermostable MutS Homolog from Thermus aquaticus.
I. Biswas and P. Hsieh (1996)
J. Biol. Chem. 271, 5040-5048
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Redundancy of Saccharomyces cerevisiae MSH3 and MSH6 in MSH2-dependent mismatch repair..
G T Marsischky, N Filosi, M F Kane, and R Kolodner (1996)
Genes & Dev. 10, 407-420
   Abstract »    PDF »
MSH5, a novel MutS homolog, facilitates meiotic reciprocal recombination between homologs in Saccharomyces cerevisiae but not mismatch repair..
N M Hollingsworth, L Ponte, and C Halsey (1995)
Genes & Dev. 9, 1728-1739
   Abstract »    PDF »
Appropriate partners make good matches.
P Karran (1995)
Science 268, 1857-1858
   PDF »
Isolation of an hMSH2-p160 heterodimer that restores DNA mismatch repair to tumor cells.
J. Drummond, G. Li, M. Longley, and P Modrich (1995)
Science 268, 1909-1912
   Abstract »    PDF »
The Saccharomyces cerevisiae Msh2 protein specifically binds to duplex oligonucleotides containing mismatched DNA base pairs and insertions..
E Alani, N W Chi, and R Kolodner (1995)
Genes & Dev. 9, 234-247
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Analysis of the Binding of p53 to DNAs Containing Mismatched and Bulged Bases.
N. Degtyareva, D. Subramanian, and J. D. Griffith (2001)
J. Biol. Chem. 276, 8778-8784
   Abstract »    Full Text »    PDF »


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