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Science 25 November 1994:
Vol. 266. no. 5189, pp. 1391 - 1395
DOI: 10.1126/science.7973731
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Articles

Science, Vol 266, Issue 5189, 1391-1395
Copyright © 1994 by American Association for the Advancement of Science

articles

The PCL2 (ORFD)-PHO85 cyclin-dependent kinase complex: a cell cycle regulator in yeast

V Measday, L Moore, J Ogas, M Tyers, and B Andrews

Department of Molecular and Medical Genetics, University of Toronto, Ontario, Canada.

Cyclin-dependent kinase (cdk) complexes are essential activators of cell cycle progression in all eukaryotes. In contrast to mammalian cells, in which multiple cdk's contribute to cell cycle regulation, the yeast cell cycle is largely controlled by the activity of a single cdk, CDC28. Analysis of the putative G1 cyclin PCL2 (ORFD) identified a second cyclin-cdk complex that contributes to cell cycle progression in yeast. PCL2 interacted with the cdk PHO85 in vivo and in vitro and formed a kinase complex that had G1-periodic activity. Under genetic conditions in which the Start transition was compromised, PHO85 and its associated cyclin subunits were essential for cell cycle commitment. Because PHO85 and another cyclin-like molecule, PHO80, also take part in inorganic phosphate metabolism, this cdk enzyme may integrate responses to nutritional conditions with the cell cycle.


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D Stuart and C Wittenberg (1995)
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Cell cycle control by a complex of the cyclin HCS26 (PCL1) and the kinase PHO85.
F. Espinoza, J Ogas, I Herskowitz, and D. Morgan (1994)
Science 266, 1388-1391
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