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Science 25 November 1994:
Vol. 266. no. 5189, pp. 1383 - 1387
DOI: 10.1126/science.7973729
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Articles

Science, Vol 266, Issue 5189, 1383-1387
Copyright © 1994 by American Association for the Advancement of Science

articles

Catalytic site components common to both splicing steps of a group II intron

G Chanfreau and A Jacquier

Unite de Genetique Moleculaire des Levures, URA 1149 du CNRS, Departement de Biologie Moleculaire, Institut Pasteur, Paris, France.

The splicing of group II introns occurs in two steps involving substrates with different chemical configurations. The question of whether these two steps are catalyzed by a single or two separate active sites is a matter of debate. Here, certain bases and phosphate oxygen atoms at conserved positions in domain V of a group II self-splicing intron are shown to be required for catalysis of both splicing steps. These results show that the active sites catalyzing the two steps must, at least, share common components, ruling out the existence of two completely distinct active sites in group II introns.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
A glimpse into the active site of a group II intron and maybe the spliceosome, too.
K. T. Dayie and R. A. Padgett (2008)
RNA 14, 1697-1703
   Abstract »    Full Text »    PDF »
Crystal Structure of a Self-Spliced Group II Intron.
N. Toor, K. S. Keating, S. D. Taylor, and A. M. Pyle (2008)
Science 320, 77-82
   Abstract »    Full Text »    PDF »
Three essential and conserved regions of the group II intron are proximal to the 5'-splice site.
A. de Lencastre and A. M. Pyle (2008)
RNA 14, 11-24
   Abstract »    Full Text »    PDF »
Fluorescence and solution NMR study of the active site of a 160-kDa group II intron ribozyme.
O. H. Gumbs, R. A. Padgett, and K. T. Dayie (2006)
RNA 12, 1693-1707
   Abstract »    Full Text »    PDF »
Structure of a self-splicing group II intron catalytic effector domain 5: Parallels with spliceosomal U6 RNA.
M. SEETHARAMAN, N. V. ELDHO, R. A. PADGETT, and K. T. DAYIE (2006)
RNA 12, 235-247
   Abstract »    Full Text »    PDF »
Group II introns as phylogenetic tools: structure, function, and evolutionary constraints.
S. A. Kelchner (2002)
Am. J. Botany 89, 1651-1669
   Abstract »    Full Text »    PDF »
Structural Insights into Group II Intron Catalysis and Branch-Site Selection.
L. Zhang and J. A. Doudna (2002)
Science 295, 2084-2088
   Abstract »    Full Text »    PDF »
Identification of specific Rp-phosphate oxygens in the tRNA anticodon loop required for ribosomal P-site binding.
W. Schnitzer and U. von Ahsen (1997)
PNAS 94, 12823-12828
   Abstract »    Full Text »    PDF »
Functionally redundant interactions between U2 and U6 spliceosomal snRNAs..
D J Field and J D Friesen (1996)
Genes & Dev. 10, 489-501
   Abstract »    PDF »


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Science. ISSN 0036-8075 (print), 1095-9203 (online)