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Science 25 November 1994:
Vol. 266. no. 5189, pp. 1380 - 1383
DOI: 10.1126/science.7973728
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Articles

Science, Vol 266, Issue 5189, 1380-1383
Copyright © 1994 by American Association for the Advancement of Science

articles

Clonal divergence in Escherichia coli as a result of recombination, not mutation

DS Guttman and DE Dykhuizen

Department of Ecology and Evolution, State University of New York, Stony Brook 11794.

Nucleotide sequence analysis was performed on 12 natural isolates of Escherichia coli in four loci located in close proximity on the chromosome. A comparison of gene genealogies indicated that three recombination events have occurred in a subset of the strains (ECOR group A) in the time since their divergence from a common ancestor, while during the same time, no mutational divergence has occurred. The common ancestor of this subset existed no more than 2400 years ago, and recombination was shown to occur at a rate of 5.0 x 10(-9) changes per nucleotide per generation--50-fold higher than the mutation rate. Thus, recombination has been the dominant force driving the clonal divergence of the ECOR group A strains and must be considered a significant factor in structuring E. coli populations.


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