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Science 18 November 1994:
Vol. 266. no. 5188, pp. 1233 - 1236
DOI: 10.1126/science.7973706
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Articles

Science, Vol 266, Issue 5188, 1233-1236
Copyright © 1994 by American Association for the Advancement of Science

articles

Circadian clock mutants of cyanobacteria

T Kondo, NF Tsinoremas, SS Golden, CH Johnson, S Kutsuna, and M Ishiura

National Institute for Basic Biology, Okazaki, Japan.

A diverse set of circadian clock mutants was isolated in a cyanobacterial strain that carries a bacterial luciferase reporter gene attached to a clock-controlled promoter. Among 150,000 clones of chemically mutagenized bioluminescent cells, 12 mutants were isolated that exhibit a broad spectrum of periods (between 16 and 60 hours), and 5 mutants were found that show a variety of unusual patterns, including arrhythmia. These mutations appear to be clock-specific. Moreover, it was demonstrated that in this cyanobacterium it is possible to clone mutant genes by complementation, which provides a means to genetically dissect the circadian mechanism.


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