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Science 11 November 1994:
Vol. 266. no. 5187, pp. 1007 - 1012
DOI: 10.1126/science.7973649
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Articles

Science, Vol 266, Issue 5187, 1007-1012
Copyright © 1994 by American Association for the Advancement of Science

articles

Isolation of components of the chloroplast protein import machinery

DJ Schnell, F Kessler, and G Blobel

Department of Biological Sciences, Rutgers, State University of New Jersey, Newark 07102.

Components of the protein import machinery of the chloroplast were isolated by a procedure in which the import machinery was engaged in vitro with a tagged import substrate under conditions that yielded largely chloroplast envelope-bound import intermediates. Subsequent detergent solubilization of envelope membranes showed that six envelope polypeptides copurified specifically and, apparently, stoichiometrically with the import intermediates. Four of these polypeptides are components of the outer membrane import machinery and are associated with early import intermediates. Two of these polypeptides have been characterized. One is a homolog of the heat shock protein hsp70; the other one is a channel-protein candidate.


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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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PNAS 96, 784-789
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B. Bolter, J. Soll, A. Schulz, S. Hinnah, and R. Wagner (1998)
PNAS 95, 15831-15836
   Abstract »    Full Text »    PDF »
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A. Kouranov, X. Chen, B. Fuks, and D. J. Schnell (1998)
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   Abstract »    Full Text »    PDF »
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Science 282, 100-103
   Abstract »    Full Text »
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   Abstract »    Full Text »
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   Abstract »    Full Text »    PDF »
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Identification of a Translocation Intermediate Occupying Functional Protein Import Sites in the Chloroplastic Envelope Membrane.
J. E. Froehlich and K. Keegstra (1997)
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M. Akita, E. Nielsen, and K. Keegstra (1997)
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D. Chen and D. J. Schnell (1997)
J. Biol. Chem. 272, 6614-6620
   Abstract »    Full Text »    PDF »
The Early Stage of Chloroplast Protein Import Involves Com70.
L. Kourtz and K. Ko (1997)
J. Biol. Chem. 272, 2808-2813
   Abstract »    Full Text »    PDF »
In Vitro Interaction between a Chloroplast Transit Peptide and Chloroplast Outer Envelope Lipids Is Sequence-specific and Lipid Class-dependent.
P. Pinnaduwage and B. D. Bruce (1996)
J. Biol. Chem. 271, 32907-32915
   Abstract »    Full Text »    PDF »
Protein Translocation at the Envelope and Thylakoid Membranes of Chloroplasts.
A. Kouranov and D. J. Schnell (1996)
J. Biol. Chem. 271, 31009-31012
   Full Text »    PDF »
A Protein Import Receptor of Chloroplasts Is Inserted into the Outer Envelope Membrane by a Novel Pathway.
E. Muckel and J. Soll (1996)
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   Abstract »    Full Text »    PDF »
Phosphorylation of the Transit Sequence of Chloroplast Precursor Proteins.
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J. Biol. Chem. 271, 6545-6554
   Abstract »    Full Text »    PDF »
Isolation and Characterization of a cDNA Clone Encoding a Member of the Com44/Cim44 Envelope Components of the Chloroplast Protein Import Apparatus.
K. Ko, D. Budd, C. Wu, F. Seibert, L. Kourtz, and Z. W. Ko (1995)
J. Biol. Chem. 270, 28601-28608
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Characterization of the Import Process of a Transit Peptide into Chloroplasts.
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A Voltage-dependent Porin-like Channel in the Inner Envelope Membrane of Plant Chloroplasts.
B. Fuks and F. Homblé (1995)
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Unidirectional and Bidirectional Protein Traffic across Membranes.
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Protein-conducting Channels for the Translocation of Proteins into and across Membranes.
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Molecular Dissection of the Mechanism of Protein Import into Chloroplasts.
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A second, substrate-dependent site of protein import into chloroplasts.
S. Reinbothe, R. Mache, and C. Reinbothe (2000)
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In Vivo Analysis of the Role of atTic20 in Protein Import into Chloroplasts.
X. Chen, M. D. Smith, L. Fitzpatrick, and D. J. Schnell (2002)
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