Related Content
Search Google Scholar for:
|
|
Science 4 November 1994:
Vol. 266. no. 5186, pp. 822 - 825
DOI: 10.1126/science.7526463
|
|
Articles
Science, Vol 266, Issue 5186, 822-825
Copyright © 1994 by American Association for the Advancement of Science
Determination of intrinsic transcription termination efficiency by RNA polymerase elongation rate
JC McDowell,
JW Roberts,
DJ Jin,
and
C Gross
Section of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, NY 14853.
Transcription terminators recognized by several RNA polymerases include a DNA segment encoding uridine-rich RNA and, for bacterial RNA polymerase, a hairpin loop located immediately upstream. Here, mutationally altered Escherichia coli RNA polymerase enzymes that have different termination efficiencies were used to show that the extent of transcription through the uridine-rich encoding segment is controlled by the substrate concentration of nucleoside triphosphate. This result implies that the rate of elongation determines the probability of transcript release. Moreover, the position of release sites suggests an important spatial relation between the RNA hairpin and the boundary of the terminator.
THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
- Monitoring RNA transcription in real time by using surface plasmon resonance.
- S. J. Greive, S. E. Weitzel, J. P. Goodarzi, L. J. Main, Z. Pasman, and P. H. von Hippel (2008)
PNAS
105, 3315-3320
| Abstract »
| Full Text »
| PDF »
- TRAP-5' stem loop interaction increases the efficiency of transcription termination in the Bacillus subtilis trpEDCFBA operon leader region.
- A. P. McGraw, P. C. Bevilacqua, and P. Babitzke (2007)
RNA
13, 2020-2033
| Abstract »
| Full Text »
| PDF »
- Allosteric control of the RNA polymerase by the elongation factor RfaH.
- V. Svetlov, G. A. Belogurov, E. Shabrova, D. G. Vassylyev, and I. Artsimovitch (2007)
Nucleic Acids Res.
35, 5694-5705
| Abstract »
| Full Text »
| PDF »
- {sigma}E Regulates and Is Regulated by a Small RNA in Escherichia coli.
- K. M. Thompson, V. A. Rhodius, and S. Gottesman (2007)
J. Bacteriol.
189, 4243-4256
| Abstract »
| Full Text »
| PDF »
- Participation of Rho-dependent transcription termination in oxidative stress sensitivity caused by an rpoB mutation.
- N. Kawamura, K. Kurokawa, T. Ito, H. Hamamoto, H. Koyama, C. Kaito, and K. Sekimizu (2005)
Genes Cells
10, 477-487
| Abstract »
| Full Text »
| PDF »
- Kinetic Analysis of tRNA-Directed Transcription Antitermination of the Bacillus subtilis glyQS Gene In Vitro.
- F. J. Grundy and T. M. Henkin (2004)
J. Bacteriol.
186, 5392-5399
| Abstract »
| Full Text »
| PDF »
- Diversity in the Rates of Transcript Elongation by Single RNA Polymerase Molecules.
- S. F. Tolic-Norrelykke, A. M. Engh, R. Landick, and J. Gelles (2004)
J. Biol. Chem.
279, 3292-3299
| Abstract »
| Full Text »
| PDF »
- RNA polymerase mutations that impair conversion to a termination-resistant complex by Q antiterminator proteins.
- T. J. Santangelo, R. A. Mooney, R. Landick, and J. W. Roberts (2003)
Genes & Dev.
17, 1281-1292
| Abstract »
| Full Text »
| PDF »
- Single molecule analysis of RNA polymerase elongation reveals uniform kinetic behavior.
- K. Adelman, A. La Porta, T. J. Santangelo, J. T. Lis, J. W. Roberts, and M. D. Wang (2002)
PNAS
99, 13538-13543
| Abstract »
| Full Text »
| PDF »
- The Downstream DNA Jaw of Bacterial RNA Polymerase Facilitates Both Transcriptional Initiation and Pausing.
- J. Ederth, I. Artsimovitch, L. A. Isaksson, and R. Landick (2002)
J. Biol. Chem.
277, 37456-37463
| Abstract »
| Full Text »
| PDF »
- NusA-stimulated RNA polymerase pausing and termination participates in the Bacillus subtilis trp operon attenuation mechanism invitro.
- A. V. Yakhnin and P. Babitzke (2002)
PNAS
99, 11067-11072
| Abstract »
| Full Text »
| PDF »
- Transfer RNA-mediated antitermination in vitro.
- H. Putzer, C. Condon, D. Brechemier-Baey, R. Brito, and M. Grunberg-Manago (2002)
Nucleic Acids Res.
30, 3026-3033
| Abstract »
| Full Text »
| PDF »
- Transcription Termination: Primary Intermediates and Secondary Adducts.
- M. Kashlev and N. Komissarova (2002)
J. Biol. Chem.
277, 14501-14508
| Abstract »
| Full Text »
| PDF »
- Conserved economics of transcription termination in eubacteria.
- S. Unniraman, R. Prakash, and V. Nagaraja (2002)
Nucleic Acids Res.
30, 675-684
| Abstract »
| Full Text »
| PDF »
- Alternate Paradigm for Intrinsic Transcription Termination in Eubacteria.
- S. Unniraman, R. Prakash, and V. Nagaraja (2001)
J. Biol. Chem.
276, 41850-41855
| Abstract »
| Full Text »
| PDF »
- Recombinant Thermus aquaticus RNA Polymerase, a New Tool for Structure-Based Analysis of Transcription.
- L. Minakhin, S. Nechaev, E. A. Campbell, and K. Severinov (2001)
J. Bacteriol.
183, 71-76
| Abstract »
| Full Text »
- RNA Polymerases from Bacillus subtilis and Escherichia coli Differ in Recognition of Regulatory Signals In Vitro.
- I. Artsimovitch, V. Svetlov, L. Anthony, R. R. Burgess, and R. Landick (2000)
J. Bacteriol.
182, 6027-6035
| Abstract »
| Full Text »
- Nonequilibrium mechanism of transcription termination from observations of single RNA polymerase molecules.
- H. Yin, I. Artsimovitch, R. Landick, and J. Gelles (1999)
PNAS
96, 13124-13129
| Abstract »
| Full Text »
| PDF »
- Altering the Intracellular Environment Increases the Frequency of Tandem Repeat Deletion during Moloney Murine Leukemia Virus Reverse Transcription.
- J. K. Pfeiffer, R. S. Topping, N.-H. Shin, and A. Telesnitsky (1999)
J. Virol.
73, 8441-8447
| Abstract »
| Full Text »
| PDF »
- Mechanism of Intrinsic Transcription Termination and Antitermination.
- W. S. Yarnell and J. W. Roberts (1999)
Science
284, 611-615
| Abstract »
| Full Text »
- Activation of Rho-dependent Transcription Termination by NusG. DEPENDENCE ON TERMINATOR LOCATION AND ACCELERATION OF RNA RELEASE.
- C. M. Burns, W. L. Nowatzke, and J. P. Richardson (1999)
J. Biol. Chem.
274, 5245-5251
| Abstract »
| Full Text »
| PDF »
- A surface of Escherichia coli sigma 70 required for promoter function and antitermination by phage lambda Q protein.
- D. C. Ko, M. T. Marr, J. Guo, and J. W. Roberts (1998)
Genes & Dev.
12, 3276-3285
| Abstract »
| Full Text »
- Interaction of a nascent RNA structure with RNA polymerase is required for hairpin-dependent transcriptional pausing but not for transcript release.
- I. Artsimovitch and R. Landick (1998)
Genes & Dev.
12, 3110-3122
| Abstract »
| Full Text »
- Recognition of a Human Arrest Site Is Conserved between RNA Polymerase II and Prokaryotic RNA Polymerases.
- J. Mote Jr. and D. Reines (1998)
J. Biol. Chem.
273, 16843-16852
| Abstract »
| Full Text »
| PDF »
- Information Processing by RNA Polymerase: Recognition of Regulatory Signals during RNA Chain Elongation.
- R. A. Mooney, I. Artsimovitch, and R. Landick (1998)
J. Bacteriol.
180, 3265-3275
| Full Text »
- Preferential interaction of the his pause RNA hairpin with RNA polymerase beta subunit residues 904-950 correlates with strong transcriptional pausing.
- D. Wang, K. Severinov, and R. Landick (1997)
PNAS
94, 8433-8438
| Abstract »
| Full Text »
| PDF »
- A Mutant RNA Polymerase Reveals a Kinetic Mechanism for the Switch between Nonproductive Stuttering Synthesis and Productive Initiation during Promoter Clearance.
- D. J. Jin and D. J. Jin (1996)
J. Biol. Chem.
271, 11659-11667
| Abstract »
| Full Text »
| PDF »
- The beta Subunit Rif-cluster I Is Only Angstroms Away from the Active Center of Escherichia coli RNA Polymerase.
- K. Severinov, A. Mustaev, E. Severinova, M. Kozlov, S. A. Darst, and A. Goldfarb (1995)
J. Biol. Chem.
270, 29428-29432
| Abstract »
| Full Text »
| PDF »
- Balanced branching in transcription termination.
- K. J. Harrington, R. B. Laughlin, and S. Liang (2001)
PNAS
98, 5019-5024
| Abstract »
| Full Text »
| PDF »
|
|
