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Science 4 November 1994:
Vol. 266. no. 5186, pp. 814 - 816
DOI: 10.1126/science.7973637
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Articles

Science, Vol 266, Issue 5186, 814-816
Copyright © 1994 by American Association for the Advancement of Science

articles

DNA loop repair by human cell extracts

A Umar, JC Boyer, and TA Kunkel

Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709.

An activity in human cell extracts is described that repairs DNA with loops of five or more unpaired bases. Repair is strand-specific and is directed by a nick located 5' or 3' to the loop. This repair is observed in a colorectal cancer cell line that is devoid of a wild-type hMLH1 gene and is deficient in repair of mismatches. However, a cell line with deletions in both hMSH2 alleles is deficient in repair of both loops and mismatches. Defects in loop repair may be relevant to the repetitive-sequence instability observed in cancers and other hereditary diseases.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
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Efficient repair of large DNA loops in Saccharomyces cerevisiae.
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Repair of Large Insertion/Deletion Heterologies in Human Nuclear Extracts Is Directed by a 5' Single-strand Break and Is Independent of the Mismatch Repair System.
S. J. Littman, W.-h. Fang, and P. Modrich (1999)
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DNA secondary structure: A common and causative factor for expansion in human disease.
C. T. McMurray (1999)
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Relative Rates of Retroviral Reverse Transcriptase Template Switching during RNA- and DNA-Dependent DNA Synthesis.
R. R. Bowman, W.-S. Hu, and V. K. Pathak (1998)
J. Virol. 72, 5198-5206
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Functional Overlap in Mismatch Repair by Human MSH3 and MSH6.
A. Umar, J. I. Risinger, W. E. Glaab, K. R. Tindall, J. C. Barrett, and T. A. Kunkel (1998)
Genetics 148, 1637-1646
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Biased Short Tract Repair of Palindromic Loop Mismatches in Mammalian Cells.
D. G. Taghian, H. Hough, and J. A. Nickoloff (1998)
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Recombination-dependent deletion formation in mammalian cells deficient in the nucleotide excision repair gene ERCC1.
R. G. Sargent, R. L. Rolig, A. E. Kilburn, G. M. Adair, J. H. Wilson, and R. S. Nairn (1997)
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Strand-specific Mismatch Repair in Mammalian Cells.
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J. Biol. Chem. 272, 24727-24730
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Methyl-directed Repair of Mismatched Small Heterologous Sequences in Cell Extracts from Escherichia coli.
W.-h. Fang, J.-Y. Wu, and M.-J. Su (1997)
J. Biol. Chem. 272, 22714-22720
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Cleavage of Insertion/Deletion Mismatches, Flap and Pseudo-Y DNA Structures by Deoxyinosine 3'-Endonuclease from Escherichia coli.
M. Yao and Y. W. Kow (1996)
J. Biol. Chem. 271, 30672-30676
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Biochemistry and genetics of eukaryotic mismatch repair..
R Kolodner (1996)
Genes & Dev. 10, 1433-1442
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Replication Protein A Confers Structure-specific Endonuclease Activities to the XPF-ERCC1 and XPG Subunits of Human DNA Repair Excision Nuclease.
T. Matsunaga, C.-H. Park, T. Bessho, D. Mu, and A. Sancar (1996)
J. Biol. Chem. 271, 11047-11050
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Requirement of the yeast RTH1 5' to 3' exonuclease for the stability of simple repetitive DNA.
R. Johnson, G. Kovvali, L Prakash, and S Prakash (1995)
Science 269, 238-240
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Isolation of an hMSH2-p160 heterodimer that restores DNA mismatch repair to tumor cells.
J. Drummond, G. Li, M. Longley, and P Modrich (1995)
Science 268, 1909-1912
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The Saccharomyces cerevisiae Msh2 protein specifically binds to duplex oligonucleotides containing mismatched DNA base pairs and insertions..
E Alani, N W Chi, and R Kolodner (1995)
Genes & Dev. 9, 234-247
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Nuclear Translocation of Mismatch Repair Proteins MSH2 and MSH6 as a Response of Cells to Alkylating Agents.
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