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Science 14 October 1994:
Vol. 266. no. 5183, pp. 282 - 285
DOI: 10.1126/science.7939665
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Articles

Science, Vol 266, Issue 5183, 282-285
Copyright © 1994 by American Association for the Advancement of Science

articles

Microtubule severing by elongation factor 1 alpha

N Shiina, Y Gotoh, N Kubomura, A Iwamatsu, and E Nishida

Department of Genetics and Molecular Biology, Kyoto University, Japan.

An activity that severs stable microtubules is thought to be involved in microtubule reorganization during the cell cycle. Here, a 48-kilodalton microtubule-severing protein was purified from Xenopus eggs and identified as translational elongation factor 1 alpha (EF-1 alpha). Bacterially expressed human EF-1 alpha also displayed microtubule-severing activity in vitro and, when microinjected into fibroblasts, induced rapid and transient fragmentation of cytoplasmic microtubule arrays. Thus, EF-1 alpha, an essential component of the eukaryotic translational apparatus, appears to have a second role as a regulator of cytoskeletal rearrangements.


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