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Science 30 September 1994:
Vol. 265. no. 5181, pp. 2096 - 2098
DOI: 10.1126/science.7522347
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Articles

Science, Vol 265, Issue 5181, 2096-2098
Copyright © 1994 by American Association for the Advancement of Science

articles

Alignment and sensitive detection of DNA by a moving interface

A Bensimon, A Simon, A Chiffaudel, V Croquette, F Heslot, and D Bensimon

Institut Pasteur, Paris, France.

In a process called "molecular combining," DNA molecules attached at one end to a solid surface were extended and aligned by a receding air-water interface and left to dry on the surface. Molecular combing was observed to extend the length of the bacteriophage lambda DNA molecule to 21.5 +/- 0.5 micrometers (unextended length, 16.2 micrometers). With the combing process, it was possible to (i) extend a chromosomal Escherichia coli DNA fragment (10(6) base pairs) and (ii) detect a minute quantity of DNA (10(3) molecules). These results open the way for a faster physical mapping of the genome and for the detection of small quantities of target DNA from a population of molecules.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
The Hsk1(Cdc7) Replication Kinase Regulates Origin Efficiency.
P. K. Patel, N. Kommajosyula, A. Rosebrock, A. Bensimon, J. Leatherwood, J. Bechhoefer, and N. Rhind (2008)
Mol. Biol. Cell 19, 5550-5558
   Abstract »    Full Text »    PDF »
See me, feel me: methods to concurrently visualize and manipulate single DNA molecules and associated proteins.
J. van Mameren, E. J. G. Peterman, and G. J. L. Wuite (2008)
Nucleic Acids Res. 36, 4381-4389
   Abstract »    Full Text »    PDF »
A comparative analysis of Dmc1 and Rad51 nucleoprotein filaments.
S. D. Sheridan, X. Yu, R. Roth, J. E. Heuser, M. G. Sehorn, P. Sung, E. H. Egelman, and D. K. Bishop (2008)
Nucleic Acids Res. 36, 4057-4066
   Abstract »    Full Text »    PDF »
Replication Fork Velocities at Adjacent Replication Origins Are Coordinately Modified during DNA Replication in Human Cells.
C. Conti, B. Sacca, J. Herrick, C. Lalou, Y. Pommier, and A. Bensimon (2007)
Mol. Biol. Cell 18, 3059-3067
   Abstract »    Full Text »    PDF »
Endogenous {gamma}-H2AX-ATM-Chk2 Checkpoint Activation in Bloom's Syndrome Helicase Deficient Cells Is Related to DNA Replication Arrested Forks.
V. A. Rao, C. Conti, J. Guirouilh-Barbat, A. Nakamura, Z.-H. Miao, S. L. Davies, B. Sacca, I. D. Hickson, A. Bensimon, and Y. Pommier (2007)
Mol. Cancer Res. 5, 713-724
   Abstract »    Full Text »    PDF »
DNA Replication Origin Interference Increases the Spacing between Initiation Events in Human Cells.
R. Lebofsky, R. Heilig, M. Sonnleitner, J. Weissenbach, and A. Bensimon (2006)
Mol. Biol. Cell 17, 5337-5345
   Abstract »    Full Text »    PDF »
DNA Replication Origins Fire Stochastically in Fission Yeast.
P. K. Patel, B. Arcangioli, S. P. Baker, A. Bensimon, and N. Rhind (2006)
Mol. Biol. Cell 17, 308-316
   Abstract »    Full Text »    PDF »
Visualization of bidirectional initiation of chromosomal DNA replication in a human cell free system.
K. Marheineke, O. Hyrien, and T. Krude (2005)
Nucleic Acids Res. 33, 6931-6941
   Abstract »    Full Text »    PDF »
DNA Replication Origin Plasticity and Perturbed Fork Progression in Human Inverted Repeats.
R. Lebofsky and A. Bensimon (2005)
Mol. Cell. Biol. 25, 6789-6797
   Abstract »    Full Text »    PDF »
Human ribosomal RNA gene arrays display a broad range of palindromic structures.
S. Caburet, C. Conti, C. Schurra, R. Lebofsky, S. J. Edelstein, and A. Bensimon (2005)
Genome Res. 15, 1079-1085
   Abstract »    Full Text »    PDF »
Independence of replisomes in Escherichia coli chromosomal replication.
A. M. Breier, H.-U. G. Weier, and N. R. Cozzarelli (2005)
PNAS 102, 3942-3947
   Abstract »    Full Text »    PDF »
Genomic Organization of Amplified MYC Genes Suggests Distinct Mechanisms of Amplification in Tumorigenesis.
J. Herrick, C. Conti, S. Teissier, F. Thierry, J. Couturier, X. Sastre-Garau, M. Favre, G. Orth, and A. Bensimon (2005)
Cancer Res. 65, 1174-1179
   Abstract »    Full Text »    PDF »
Sequence-specific fluorescent labeling of double-stranded DNA observed at the single molecule level.
B. Geron-Landre, T. Roulon, P. Desbiolles, and C. Escude (2003)
Nucleic Acids Res. 31, e125
   Abstract »    Full Text »    PDF »
Bar code screening on combed DNA for large rearrangements of the BRCA1 and BRCA2 genes in French breast cancer families.
S Gad, M Klinger, V Caux-Moncoutier, S Pages-Berhouet, M Gauthier-Villars, I Coupier, A Bensimon, A Aurias, and D Stoppa-Lyonnet (2002)
J. Med. Genet. 39, 817-821
   Full Text »    PDF »
Observation by fluorescence microscopy of transcription on single combed DNA.
Z. Gueroui, C. Place, E. Freyssingeas, and B. Berge (2002)
PNAS 99, 6005-6010
   Abstract »    Full Text »    PDF »
Behavior of DNA fibers stretched by precise meniscus motion control.
K. Otobe and T. Ohtani (2001)
Nucleic Acids Res. 29, e109
   Abstract »    Full Text »    PDF »
DNA Fiber Mapping Techniques for the Assembly of High-resolution Physical Maps.
H.-U. G. Weier (2001)
J. Histochem. Cytochem. 49, 939-948
   Abstract »    Full Text »    PDF »
Identification of a large rearrangement of the BRCA1 gene using colour bar code on combed DNA in an American breast/ovarian cancer family previously studied by direct sequencing.
S. Gad, M. T Scheuner, S. Pages-Berhouet, V. Caux-Moncoutier, A. Bensimon, A. Aurias, M. Pinto, and D. Stoppa-Lyonnet (2001)
J. Med. Genet. 38, 388-392
   Full Text »
Monitoring S phase progression globally and locally using BrdU incorporation in TK+ yeast strains.
A. Lengronne, P. Pasero, A. Bensimon, and E. Schwob (2001)
Nucleic Acids Res. 29, 1433-1442
   Abstract »    Full Text »    PDF »
Proximity-Induced Superconductivity in DNA.
A. Yu. Kasumov, M. Kociak, S. Guéron, B. Reulet, V. T. Volkov, D. V. Klinov, and H. Bouchiat (2001)
Science 291, 280-282
   Abstract »    Full Text »
From Micro- to Nanofabrication with Soft Materials.
S. R. Quake and A. Scherer (2000)
Science 290, 1536-1540
   Abstract »    Full Text »
DNA Fiber-FISH Staining Mechanism.
F. M. van de Rijke, R. J. Florijn, H. J. Tanke, and A. K. Raap (2000)
J. Histochem. Cytochem. 48, 743-746
   Abstract »    Full Text »
Encephalitozoon cuniculi (Microspora) genome: physical map and evidence for telomere-associated rDNA units on all chromosomes.
J.-F. Brugere, E. Cornillot, G. Metenier, A. Bensimon, and C. P. Vivares (2000)
Nucleic Acids Res. 28, 2026-2033
   Abstract »    Full Text »    PDF »
Quantifying single gene copy number by measuring fluorescent probe lengths on combed genomic DNA.
J. Herrick, X. Michalet, C. Conti, C. Schurra, and A. Bensimon (2000)
PNAS 97, 222-227
   Abstract »    Full Text »    PDF »
Genomic Organization of the S Locus: Identification and Characterization of Genes in SLG/SRK Region of S9 Haplotype of Brassica campestris (syn. rapa).
G. Suzuki, N. Kai, T. Hirose, K. Fukui, T. Nishio, S. Takayama, A. Isogai, M. Watanabe, and K. Hinata (1999)
Genetics 153, 391-400
   Abstract »    Full Text »
Automated high resolution optical mapping using arrayed, fluid-fixed DNA molecules.
J. Jing, J. Reed, J. Huang, X. Hu, V. Clarke, J. Edington, D. Housman, T. S. Anantharaman, E. J. Huff, B. Mishra, et al. (1998)
PNAS 95, 8046-8051
   Abstract »    Full Text »    PDF »
Use of a Mycobacterium tuberculosis H37Rv Bacterial Artificial Chromosome Library for Genome Mapping, Sequencing, and Comparative Genomics.
R. Brosch, S. V. Gordon, A. Billault, T. Garnier, K. Eiglmeier, C. Soravito, B. G. Barrell, and S. T. Cole (1998)
Infect. Immun. 66, 2221-2229
   Abstract »    Full Text »    PDF »
Local DNA stretching mimics the distortion caused by the TATA box-binding protein.
A. Lebrun, Z. Shakked, and R. Lavery (1997)
PNAS 94, 2993-2998
   Abstract »    Full Text »    PDF »
Adenosine Modulates N-Methyl-D-Aspartate–Stimulated Hippocampal Nitric Oxide Production In Vivo.
A. Bhardwaj, F. J. Northington, R. C. Koehler, T. Stiefel, D. F. Hanley, and R. J. Traystman (1995)
Stroke 26, 1627-1633
   Abstract »    Full Text »
Stretching of a single tethered polymer in a uniform flow.
T. Perkins, D. Smith, R. Larson, and S Chu (1995)
Science 268, 83-87
   Abstract »    PDF »
Aphidicolin Triggers a Block to Replication Origin Firing in Xenopus Egg Extracts.
K. Marheineke and O. Hyrien (2001)
J. Biol. Chem. 276, 17092-17100
   Abstract »    Full Text »    PDF »


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