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Science 30 September 1994:
Vol. 265. no. 5181, pp. 2085 - 2088
DOI: 10.1126/science.7522346
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Articles

Science, Vol 265, Issue 5181, 2085-2088
Copyright © 1994 by American Association for the Advancement of Science

articles

Padlock probes: circularizing oligonucleotides for localized DNA detection

M Nilsson, H Malmgren, M Samiotaki, M Kwiatkowski, BP Chowdhary, and U Landegren

Beijer Laboratory, Department of Medical Genetics, Biomedical Center, Uppsala, Sweden.

Nucleotide sequence information derived from DNA segments of the human and other genomes is accumulating rapidly. However, it frequently proves difficult to use such short DNA segments to identify clones in genomic libraries or fragments in blots of the whole genome or for in situ analysis of chromosomes. Oligonucleotide probes, consisting of two target-complementary segments, connected by a linker sequence, were designed. Upon recognition of the specific nucleic acid molecule the ends of the probes were joined through the action of a ligase, creating circular DNA molecules catenated to the target sequence. These probes thus provide highly specific detection with minimal background.


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