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Science 30 September 1994:
Vol. 265. no. 5181, pp. 2082 - 2085
DOI: 10.1126/science.8091230
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Articles

Science, Vol 265, Issue 5181, 2082-2085
Copyright © 1994 by American Association for the Advancement of Science

articles

Specific cleavage of model recombination and repair intermediates by the yeast Rad1-Rad10 DNA endonuclease

AJ Bardwell, L Bardwell, AE Tomkinson, and EC Friedberg

Laboratory of Molecular Pathology, University of Texas Southwestern Medical Center at Dallas 75235.

The RAD1 and RAD10 genes of Saccharomyces cerevisiae are required for both nucleotide excision repair and certain mitotic recombination events. Here, model recombination and repair intermediates were used to show that Rad1-Rad10-mediated cleavage occurs at duplex-single-strand junctions. Moreover, cleavage occurs only on the strand containing the 3' single-stranded tail. Thus, both biochemical and genetic evidence indicate a role for the Rad1-Rad10 complex in the cleavage of specific recombination intermediates. Furthermore, these data suggest that Rad1-Rad10 endonuclease incises DNA 5' to damaged bases during nucleotide excision repair.


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Affinity Purification and Partial Characterization of a Yeast Multiprotein Complex for Nucleotide Excision Repair Using Histidine-tagged Rad14 Protein.
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Physical interaction between components of DNA mismatch repair and nucleotide excision repair.
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DNA Structural Elements Required for ERCC1-XPF Endonuclease Activity.
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DNA Mismatch Repair Catalyzed by Extracts of Mitotic, Postmitotic, and Senescent Drosophila Tissues and Involvement of mei-9 Gene Function for Full Activity.
A. Bhui-Kaur, M. F. Goodman, and J. Tower (1998)
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Role of Saccharomyces cerevisiae Msh2 and Msh3 repair proteins in double-strand break-induced recombination.
N. Sugawara, F. Paques, M. Colaiacovo, and J. E. Haber (1997)
PNAS 94, 9214-9219
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Ku Selectively Transfers between DNA Molecules with Homologous Ends.
T. M. Bliss and D. P. Lane (1997)
J. Biol. Chem. 272, 5765-5773
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Formation of DNA Repair Intermediates and Incision by the ATP-dependent UvrB-UvrC Endonuclease.
Y. Zou, R. Walker, H. Bassett, N. E. Geacintov, and B. Van Houten (1997)
J. Biol. Chem. 272, 4820-4827
   Abstract »    Full Text »    PDF »
Identification of Functional Domains within the RAD1·RAD10 Repair and Recombination Endonuclease of Saccharomyces cerevisiae.
K. Rodriguez, Z. Wang, E. C. Friedberg, and A. E. Tomkinson (1996)
J. Biol. Chem. 271, 20551-20558
   Abstract »    Full Text »    PDF »
A novel mechanism for telomere size control in Saccharomyces cerevisiae..
B Li and A J Lustig (1996)
Genes & Dev. 10, 1310-1326
   Abstract »    PDF »
Replication Protein A Confers Structure-specific Endonuclease Activities to the XPF-ERCC1 and XPG Subunits of Human DNA Repair Excision Nuclease.
T. Matsunaga, C.-H. Park, T. Bessho, D. Mu, and A. Sancar (1996)
J. Biol. Chem. 271, 11047-11050
   Abstract »    Full Text »    PDF »
Reconstitution of TFIIH and Requirement of Its DNA Helicase Subunits, Rad3 and Rad25, in the Incision Step of Nucleotide Excision Repair.
P. Sung, S. N. Guzder, L. Prakash, and S. Prakash (1996)
J. Biol. Chem. 271, 10821-10826
   Abstract »    Full Text »    PDF »
Analysis of Incision Sites Produced by Human Cell Extracts and Purified Proteins during Nucleotide Excision Repair of a 1,3-Intrastrand d(GpTpG)-Cisplatin Adduct.
J. G. Moggs, K. J. Yarema, J. M. Essigmann, and R. D. Wood (1996)
J. Biol. Chem. 271, 7177-7186
   Abstract »    Full Text »    PDF »
Structure-specific Nuclease Activity in Yeast Nucleotide Excision Repair Protein Rad2.
Y. Habraken, P. Sung, L. Prakash, and S. Prakash (1995)
J. Biol. Chem. 270, 30194-30198
   Abstract »    Full Text »    PDF »
Role of the Rad1 and Rad10 Proteins in Nucleotide Excision Repair and Recombination.
A. A. Davies, E. C. Friedberg, A. E. Tomkinson, R. D. Wood, and S. C. West (1995)
J. Biol. Chem. 270, 24638-24641
   Abstract »    Full Text »    PDF »
Purification and Characterization of the XPF-ERCC1 Complex of Human DNA Repair Excision Nuclease.
C.-H. Park, T. Bessho, T. Matsunaga, and A. Sancar (1995)
J. Biol. Chem. 270, 22657-22660
   Abstract »    Full Text »    PDF »
Human DNA Repair Excision Nuclease.
T. Matsunaga, D. Mu, C.-H. Park, J. T. Reardon, and A. Sancar (1995)
J. Biol. Chem. 270, 20862-20869
   Abstract »    Full Text »    PDF »
DNA Structural Elements Required for FEN-1 Binding.
J. J. Harrington and M. R. Lieber (1995)
J. Biol. Chem. 270, 4503-4508
   Abstract »    Full Text »    PDF »
The General Transcription-Repair Factor TFIIH Is Recruited to the Excision Repair Complex by the XPA Protein Independent of the TFIIE Transcription Factor.
C.-H. Park, D. Mu, J. T. Reardon, and A. Sancar (1995)
J. Biol. Chem. 270, 4896-4902
   Abstract »    Full Text »    PDF »
Mechanisms of DNA excision repair.
A Sancar (1994)
Science 266, 1954-1956
   PDF »
Repair of an Interstrand DNA Cross-link Initiated by ERCC1-XPF Repair/Recombination Nuclease.
I. Kuraoka, W. R. Kobertz, R. R. Ariza, M. Biggerstaff, J. M. Essigmann, and R. D. Wood (2000)
J. Biol. Chem. 275, 26632-26636
   Abstract »    Full Text »    PDF »
Transcription-induced Cleavage of Immunoglobulin Switch Regions by Nucleotide Excision Repair Nucleases in Vitro.
M. Tian and F. W. Alt (2000)
J. Biol. Chem. 275, 24163-24172
   Abstract »    Full Text »    PDF »


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