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Science 9 September 1994:
Vol. 265. no. 5178, pp. 1590 - 1593
DOI: 10.1126/science.8079173
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Articles

Science, Vol 265, Issue 5178, 1590-1593
Copyright © 1994 by American Association for the Advancement of Science

articles

Analysis of sequence transfers resembling gene conversion in a mouse antibody transgene

B Xu and E Selsing

Department of Pathology, Sackler Graduate School of Biomedical Science, Tufts University School of Medicine, Boston, MA 02111.

The role of gene conversion in murine immunoglobulin gene diversification is unclear. An antibody gene construct designed to provide the homologous donor and acceptor sequences required for conversion mechanisms was produced and used to generate transgenic mice. When these transgenic mice were immunized, DNA sequence transfers between tandem transgene VDJ regions were detectable and resembled gene conversion events. There is a strong link between these conversion-like sequence transfers and transgene somatic hypermutation, suggesting that both processes might occur at the same stage of B cell differentiation.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Sequence Transfers between Variable Regions in a Mouse Antibody Transgene Can Occur by Gene Conversion.
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Antibody Repertoire Development in Fetal and Neonatal Pigs. VII. Characterization of the Preimmune {kappa} Light Chain Repertoire.
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J. Immunol. 173, 6794-6805
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Cis-acting regulatory sequences promote high-frequency gene conversion between repeated sequences in mammalian cells.
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Gene Conversion-Like Sequence Transfers Between Transgenic Antibody V Genes Are Independent of RAD54.
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Alternative Splicing and Hypermutation of a Nonproductively Rearranged TCR {alpha}-Chain in a T Cell Hybridoma.
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A {lambda} 3' Enhancer Drives Active and Untemplated Somatic Hypermutation of a {lambda}1 Transgene.
Q. Kong, L. Zhao, S. Subbaiah, and N. Maizels (1998)
J. Immunol. 161, 294-301
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Hypermutation of Immunoglobulin Genes in Memory B Cells of DNA Repair-deficient Mice.
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