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Science 9 September 1994:
Vol. 265. no. 5178, pp. 1580 - 1582
DOI: 10.1126/science.8079172
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Articles

Science, Vol 265, Issue 5178, 1580-1582
Copyright © 1994 by American Association for the Advancement of Science

articles

Detection of endogenous malondialdehyde-deoxyguanosine adducts in human liver

AK Chaudhary, M Nokubo, GR Reddy, SN Yeola, JD Morrow, IA Blair, and LJ Marnett

A. B. Hancock Jr. Memorial Laboratory for Cancer Research, Vanderbilt University School of Medicine, Nashville, TN 37232-0146.

Endogenous DNA adducts may contribute to the etiology of human genetic disease and cancer. One potential source of endogenous DNA adducts is lipid peroxidation, which generates mutagenic carbonyl compounds such as malondialdehyde. A sensitive mass spectrometric method permitted detection and quantitation of the major malondialdehyde-DNA adduct, a pyrimidopurinone derived from deoxyguanosine. DNA from disease-free human liver was found to contain 5400 adducts per cell, a frequency comparable to that of adducts formed by exogenous carcinogens.


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