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Science 2 September 1994:
Vol. 265. no. 5177, pp. 1439 - 1441
DOI: 10.1126/science.8073285
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Articles

Science, Vol 265, Issue 5177, 1439-1441
Copyright © 1994 by American Association for the Advancement of Science

articles

Redistribution of intracellular Ca2+ stores during phagocytosis in human neutrophils

O Stendahl, KH Krause, J Krischer, P Jerstrom, JM Theler, RA Clark, JL Carpentier, and DP Lew

Department of Medical Microbiology, Linkoping University, Sweden.

Subcellular gradients of cytosolic free Ca2+ concentration, [Ca2+]i, are thought to be critical for the localization of functional responses within a cell. A potential but previously unexplored mechanism for the generation of gradients of [Ca2+]i is the accumulation of Ca2+ stores at the site of Ca2+ action. The distribution of the Ca2+ store markers Ca(2+)-dependent adenosine triphosphatase and calreticulin was investigated in resting and phagocytosing human neutrophils. Both proteins showed an evenly distributed fine granular pattern in nonphagocytosing cells, but became markedly concentrated in the filamentous actin-rich cytoplasmic area around the ingested particle during phagocytosis. This redistribution began at early stages of phagocytosis and did not depend on an increase in [Ca2+]i. Thus, accumulation of Ca2+ stores in a restricted area of the cell may contribute to the generation of localized increases in [Ca2+]i.


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