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Science 26 August 1994:
Vol. 265. no. 5176, pp. 1241 - 1243
DOI: 10.1126/science.8066464
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Articles

Science, Vol 265, Issue 5176, 1241-1243
Copyright © 1994 by American Association for the Advancement of Science

articles

Catalysis of ATP-dependent homologous DNA pairing and strand exchange by yeast RAD51 protein

P Sung

Sealy Center for Molecular Science, University of Texas Medical Branch at Galveston 77555-1061.

The RAD51 gene of Saccharomyces cerevisiae is required for genetic recombination and DNA double-strand break repair. Here it is demonstrated that RAD51 protein pairs circular viral single-stranded DNA from phi X 174 or M13 with its respective homologous linear double-stranded form. The product of synapsis between these DNA partners is further processed by RAD51 to yield nicked circular duplex DNA, which indicates that RAD51 can catalyze strand exchange. The pairing and strand exchange reaction requires adenosine triphosphate, a result consistent with the presence of a DNA-dependent adenosine triphosphatase activity in RAD51 protein. Thus, RAD51 is a eukaryotic recombination protein that can catalyze the strand exchange reaction.


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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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J. Biol. Chem. 278, 35979-35987
   Abstract »    Full Text »    PDF »
Fission yeast Rhp51 is required for the maintenance of telomere structure in the absence of the Ku heterodimer.
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