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Science 26 August 1994:
Vol. 265. no. 5176, pp. 1221 - 1225
DOI: 10.1126/science.8066460
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Articles

Science, Vol 265, Issue 5176, 1221-1225
Copyright © 1994 by American Association for the Advancement of Science

articles

Dependence of enhancer-mediated transcription of the immunoglobulin mu gene on nuclear matrix attachment regions

WC Forrester, C van Genderen, T Jenuwein, and R Grosschedl

Department of Microbiology and Immunology, University of California at San Francisco (UCSF) 94143-0414.

Transcription of the immunoglobulin mu heavy chain locus is regulated by an intronic enhancer that is flanked on both sides by nuclear matrix attachment regions (MARs). These MARs have now been shown to be essential for transcription of a rearranged mu gene in transgenic B lymphocytes, but they were not required in stably transfected tissue culture cells. Normal rates of transcriptional initiation at a variable region promoter and the formation of an extended deoxyribonuclease I (DNase I)--sensitive chromatin domain were dependent on MARs, although DNase I hypersensitivity at the enhancer was detected in the absence of MARs. Thus, transcriptional activation of the mu gene during normal lymphoid development requires a synergistic collaboration between the enhancer and flanking MARs.


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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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   Abstract »    PDF »
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   Abstract »    PDF »
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