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Science 12 August 1994:
Vol. 265. no. 5174, pp. 952 - 954
DOI: 10.1126/science.8052852
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Articles

Science, Vol 265, Issue 5174, 952-954
Copyright © 1994 by American Association for the Advancement of Science

articles

Bidirectional replication from an internal origin in a linear streptomyces plasmid

PC Chang and SN Cohen

Department of Genetics, Stanford University School of Medicine, CA 94305.

Commonly, linear replicons that have protein covalently attached to 5' DNA termini replicate by protein-primed, strand-displacing, continuous synthesis of full-length strands. The synthesis of DNA in pSLA2, a 17-kilobase linear plasmid of Streptomyces rochei containing 5' terminal protein, occurs bidirectionally from an internally located replication origin. The replication intermediates are linear duplex molecules that have recessed (approximately 280 nucleotides) 5' ends rather than full-length single strands. The 3' over-hangs may serve as templates for the non-displacing synthesis of the lagging strand terminus primed by the covalently attached 5' DNA binding protein.


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Terminal proteins essential for the replication of linear plasmids and chromosomes in Streptomyces.
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