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Science 5 August 1994:
Vol. 265. no. 5173, pp. 762 - 765
DOI: 10.1126/science.265.5173.762
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Articles

Antibody-Mediated Bacteriorhodopsin Orientation for Molecular Device Architectures

Koichi Koyama 1, Naoto Yamaguchi 1, and Tsutomu Miyasaka 1

1 Ashigara Research Laboratories, Fuji Photo Film Co. Ltd., Minamiashigara, Kanagawa, 250-01, Japan

A rational method for constructing highly oriented films of purple membrane (PM) has been established by using two kinds of bispecific antibodies with different antigen-binding sites, one binding to a specific side of bacteriorhodopsin and the other to a phospholipid hapten. A hapten monolayer deposited on a metal electrode was treated with a bispecific antibody solution and incubated with a PM suspension to produce a highly oriented PM film, as confirmed by electron microscopy in which an immunogold labeling technique was used. This antibody-mediated PM monolayer was then used in the construction of a light-sensing photoelectric device. A comparison of the two incorporated PM monolayers showed that highly efficient photocurrents were produced by the PM monolayer whose unidirectionally oriented cytoplasmic surface faces the electrode.

Submitted on March 18, 1994
Accepted on May 31, 1994


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