Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 15 July 1994:
Vol. 265. no. 5170, pp. 364 - 367
DOI: 10.1126/science.265.5170.364
Prev | Table of Contents | Next

Articles

Alterations of Single Molecule Fluorescence Lifetimes in Near-Field Optical Microscopy

W. Patrick Ambrose 1, Peter M. Goodwin 1, Richard A. Keller 1, and John C. Martin 2

1 Chemical Science and Technology Division, Los Alamos National Laboratory, Los Alamos, NM 87545, USA.
2 Life Sciences Division, Los Alamos National Laboratory, Los Alamos, NM 87545, USA.

Fluorescence lifetimes of single Rhodamine 6G molecules on silica surfaces were measured with pulsed laser excitation, time-correlated single photon counting, and near-field scanning optical microscopy (NSOM). The fluorescence lifetime varies with the position of a molecule relative to a near-field probe. Qualitative features of lifetime decreases are consistent with molecular excited state quenching effects near metal surfaces. The technique of NSOM provides a means of altering the environment of a single fluorescent molecule and its decay kinetics in a repeatable fashion.

Submitted on March 9, 1994
Accepted on May 31, 1994


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Probing conformational dynamics in single donor-acceptor synthetic molecules by means of photoinduced reversible electron transfer.
M. Cotlet, S. Masuo, G. Luo, J. Hofkens, M. Van der Auweraer, J. Verhoeven, K. Mullen, X. S. Xie, and F. De Schryver (2004)
PNAS 101, 14343-14348
   Abstract »    Full Text »    PDF »
Protein Conformational Dynamics Probed by Single-Molecule Electron Transfer.
H. Yang, G. Luo, P. Karnchanaphanurach, T.-M. Louie, I. Rech, S. Cova, L. Xun, and X. S. Xie (2003)
Science 302, 262-266
   Abstract »    Full Text »    PDF »
Cell biology beyond the diffraction limit: near-field scanning optical microscopy.
F. de Lange, A. Cambi, R. Huijbens, B. de Bakker, W. Rensen, M. Garcia-Parajo, N. van Hulst, and C. G. Figdor (2001)
J. Cell Sci. 114, 4153-4160
   Abstract »    Full Text »    PDF »
Fluorescence Spectroscopy of Single Biomolecules.
S. Weiss (1999)
Science 283, 1676-1683
   Abstract »    Full Text »
Fluorescence and photobleaching dynamics of single light-harvesting complexes.
M. A. Bopp, Y. Jia, L. Li, R. J. Cogdell, and R. M. Hochstrasser (1997)
PNAS 94, 10630-10635
   Abstract »    Full Text »    PDF »
Conformational fluctuations in single DNA molecules.
S. Wennmalm, L. Edman, and R. Rigler (1997)
PNAS 94, 10641-10646
   Abstract »    Full Text »    PDF »
Nonexponential kinetics of a single tRNAPhe molecule under physiological conditions.
Y. Jia, A. Sytnik, L. Li, S. Vladimirov, B. S. Cooperman, and R. M. Hochstrasser (1997)
PNAS 94, 7932-7936
   Abstract »    Full Text »    PDF »
Observation of Individual Chemical Reactions in Solution.
M. M. Collinson and R. M. Wightman (1995)
Science 268, 1883-1885
   Abstract »    PDF »
Probing individual molecules with confocal fluorescence microscopy.
S Nie, D. Chiu, and R. Zare (1994)
Science 266, 1018-1021
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)