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Science 8 July 1994:
Vol. 265. no. 5169, pp. 241 - 244
DOI: 10.1126/science.7912851
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Articles

Science, Vol 265, Issue 5169, 241-244
Copyright © 1994 by American Association for the Advancement of Science

articles

Gem: an induced, immediate early protein belonging to the Ras family

J Maguire, T Santoro, P Jensen, U Siebenlist, J Yewdell, and K Kelly

Laboratory of Pathology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892.

A gene encoding a 35-kilodalton guanosine triphosphate (GTP)-binding protein, Gem, was cloned from mitogen-induced human peripheral blood T cells. Gem and Rad, the product of a gene overexpressed in skeletal muscle in individuals with Type II diabetes, constitute a new family of Ras-related GTP-binding proteins. The distinct structural features of this family include the G3 GTP-binding motif, extensive amino- and carboxyl-terminal extensions beyond the Ras-related domain, and a motif that determines membrane association. Gem was transiently expressed in human peripheral blood T cells in response to mitogenic stimulation; the protein was phosphorylated on tyrosine residues and localized to the cytosolic face of the plasma membrane. Deregulated Gem expression prevented proliferation of normal and transformed 3T3 cells. These results suggest that Gem is a regulatory protein, possibly participating in receptor-mediated signal transduction at the plasma membrane.


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