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Science 1 July 1994:
Vol. 265. no. 5168, pp. 103 - 106
DOI: 10.1126/science.8016642
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Articles

Science, Vol 265, Issue 5168, 103-106
Copyright © 1994 by American Association for the Advancement of Science

articles

Deletion of a DNA polymerase beta gene segment in T cells using cell type-specific gene targeting

H Gu, JD Marth, PC Orban, H Mossmann, and K Rajewsky

Institute for Genetics, University of Cologne, Germany.

Deletion of the promoter and the first exon of the DNA polymerase beta gene (pol beta) in the mouse germ line results in a lethal phenotype. With the use of the bacteriophage-derived, site-specific recombinase Cre in a transgenic approach, the same mutation can be selectively introduced into a particular cellular compartment-in this case, T cells. The impact of the mutation on those cells can then be analyzed because the mutant animals are viable.


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Mol. Cell. Biol. 23, 908-915
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Signal-Transducing Adaptor Molecules STAM1 and STAM2 Are Required for T-Cell Development and Survival.
M. Yamada, N. Ishii, H. Asao, K. Murata, C. Kanazawa, H. Sasaki, and K. Sugamura (2002)
Mol. Cell. Biol. 22, 8648-8658
   Abstract »    Full Text »    PDF »
Lineage-specific requirements of {beta}-catenin in neural crest development.
L. Hari, V. Brault, M. Kleber, H.-Y. Lee, F. Ille, R. Leimeroth, C. Paratore, U. Suter, R. Kemler, and L. Sommer (2002)
J. Cell Biol. 159, 867-880
   Abstract »    Full Text »    PDF »
Tools for targeted manipulation of the mouse genome.
L. van der Weyden, D. J. Adams, and A. Bradley (2002)
Physiol Genomics 11, 133-164
   Abstract »    Full Text »    PDF »
Inactivation of the F4/80 Glycoprotein in the Mouse Germ Line.
E. Schaller, A. J. Macfarlane, R. A. Rupec, S. Gordon, A. J. McKnight, and K. Pfeffer (2002)
Mol. Cell. Biol. 22, 8035-8043
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Loss of Brca2 and p53 Synergistically Promotes Genomic Instability and Deregulation of T-cell Apoptosis.
A. M. Y. Cheung, M. P. Hande, F. Jalali, M.-S. Tsao, B. Skinnider, A. Hirao, J. P. McPherson, J. Karaskova, A. Suzuki, A. Wakeham, et al. (2002)
Cancer Res. 62, 6194-6204
   Abstract »    Full Text »    PDF »
The transcription factor Sox9 has essential roles in successive steps of the chondrocyte differentiation pathway and is required for expression of Sox5 and Sox6.
H. Akiyama, M.-C. Chaboissier, J. F. Martin, A. Schedl, and B. de Crombrugghe (2002)
Genes & Dev. 16, 2813-2828
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Mice Lacking the EDB Segment of Fibronectin Develop Normally but Exhibit Reduced Cell Growth and Fibronectin Matrix Assembly in Vitro.
T. Fukuda, N. Yoshida, Y. Kataoka, R.-i. Manabe, Y. Mizuno-Horikawa, M. Sato, K. Kuriyama, N. Yasui, and K. Sekiguchi (2002)
Cancer Res. 62, 5603-5610
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Threonine 79 Is a Hinge Residue That Governs the Fidelity of DNA Polymerase beta by Helping to Position the DNA within the Active Site.
M. Maitra, A. Gudzelak Jr., S.-X. Li, Y. Matsumoto, K. A. Eckert, J. Jager, and J. B. Sweasy (2002)
J. Biol. Chem. 277, 35550-35560
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Smooth muscle expression of Cre recombinase and eGFP in transgenic mice.
H.-B. Xin, K.-Y. Deng, M. Rishniw, G. Ji, and M. I. Kotlikoff (2002)
Physiol Genomics 10, 211-215
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Induction of DNA polymerase {beta}-dependent base excision repair in response to oxidative stress in vivo.
D. C. Cabelof, J. J. Raffoul, S. Yanamadala, Z. Guo, and A. R. Heydari (2002)
Carcinogenesis 23, 1419-1425
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Impairment of L-type Ca2+ Channel-Dependent Forms of Hippocampal Synaptic Plasticity in Mice Deficient in the Extracellular Matrix Glycoprotein Tenascin-C.
M. R. Evers, B. Salmen, O. Bukalo, A. Rollenhagen, M. R. Bosl, F. Morellini, U. Bartsch, A. Dityatev, and M. Schachner (2002)
J. Neurosci. 22, 7177-7194
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Induced disruption of the transforming growth factor beta type II receptor gene in mice causes a lethal inflammatory disorder that is transplantable.
P. Leveen, J. Larsson, M. Ehinger, C. M. Cilio, M. Sundler, L. J. Sjostrand, R. Holmdahl, and S. Karlsson (2002)
Blood 100, 560-568
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Directed evolution of the site specificity of Cre recombinase.
S. W. Santoro and P. G. Schultz (2002)
PNAS 99, 4185-4190
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Ability of the hydrophobic FGF and basic TAT peptides to promote cellular uptake of recombinant Cre recombinase: A tool for efficient genetic engineering of mammalian genomes.
M. Peitz, K. Pfannkuche, K. Rajewsky, and F. Edenhofer (2002)
PNAS 99, 4489-4494
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