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Science 24 June 1994:
Vol. 264. no. 5167, pp. 1936 - 1938
DOI: 10.1126/science.8009224

Articles

Science, Vol 264, Issue 5167, 1936-1938
Copyright © 1994 by American Association for the Advancement of Science


articles

Maternal rescue of transforming growth factor-beta 1 null mice

JJ Letterio, AG Geiser, AB Kulkarni, NS Roche, MB Sporn, and AB Roberts

Laboratory of Chemoprevention, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892.

Maternal sources of transforming growth factor-beta 1 (TGF-beta 1) are shown here to contribute to the normal appearance and perinatal survival of TGF-beta 1 null newborn mice. Labeled TGF-beta 1 crossed the placenta and was recovered intact from various tissues after oral administration to mouse pups. TGF beta-1 protein was also detected in cells recovered from breast milk. In immunohistochemical analyses, TGF-beta 1 null embryos and null newborn pups born to TGF-beta 1 heterozygotes stained positive for TGF-beta 1, whereas those born to a null female were negative and had severe cardiac abnormalities. These results suggest an important role for maternal sources of TGF-beta 1 during development and, more generally, provide evidence for maternal rescue of targeted gene disruption in the fetus.


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