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Science 24 June 1994:
Vol. 264. no. 5167, pp. 1924 - 1927
DOI: 10.1126/science.8009223
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Articles

Science, Vol 264, Issue 5167, 1924-1927
Copyright © 1994 by American Association for the Advancement of Science

articles

Expanding the scope of RNA catalysis

Prudent JR, T Uno, and PG Schultz

Department of Chemistry, University of California, Berkeley.

The basic notions of transition state theory have been exploited in the past to generate highly selective catalysts from the vast library of antibody molecules in the immune system. These same ideas were used to isolate an RNA molecule, from a large library of RNAs, that catalyzes the isomerization of a bridged biphenyl. The RNA-catalyzed reaction displays Michaelis-Menten kinetics with a catalytic rate constant (kcat) of 2.8 x 10(-5) per minute and a Michaelis constant (Km) of 542 microM; the reaction is competitively inhibited by the planar transition state analog with an inhibition constant (Ki) value of approximately 7 microM. This approach may provide a general strategy for expanding the scope of RNA catalysis beyond those reactions in which the substrates are nucleic acids or nucleic acid derivatives.


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