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Science 17 June 1994:
Vol. 264. no. 5166, pp. 1762 - 1764
DOI: 10.1126/science.8209256
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Articles

Science, Vol 264, Issue 5166, 1762-1764
Copyright © 1994 by American Association for the Advancement of Science

articles

Attenuation of fungal virulence by synthetic infectious hypovirus transcripts

B Chen, GH Choi, and DL Nuss

Roche Institute of Molecular Biology, Roche Research Center, Nutley, NJ 07110.

Noninfectious, cytoplasmically transmissible viral double-stranded RNAs of the genus Hypovirus cause reduced virulence (hypovirulence) in the chestnut blight fungus Cryphonectria parasitica, providing the basis for virus-mediated biological control of a fungal disease. Synthetic transcripts corresponding to a full-length hypovirus RNA coding strand are infectious when introduced into fungal spheroplasts by electroporation. Hypovirus infections were readily established in Cryphonectria parasitica and in related fungal species not previously reported to harbor viruses. These results demonstrate the use of a synthetic mycovirus transcript to expand fungal host range, thereby broadening the potential application of virus-mediated hypovirulence to control fungal pathogenesis.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Coupled termination/reinitiation for translation of the downstream open reading frame B of the prototypic hypovirus CHV1-EP713.
L.-h. Guo, L. Sun, S. Chiba, H. Araki, and N. Suzuki (2009)
Nucleic Acids Res. 37, 3645-3659
   Abstract »    Full Text »    PDF »
Hypovirus-Responsive Transcription Factor Gene pro1 of the Chestnut Blight Fungus Cryphonectria parasitica Is Required for Female Fertility, Asexual Spore Development, and Stable Maintenance of Hypovirus Infection.
Q. Sun, G. H. Choi, and D. L. Nuss (2009)
Eukaryot. Cell 8, 262-270
   Abstract »    Full Text »    PDF »
A host dicer is required for defective viral RNA production and recombinant virus vector RNA instability for a positive sense RNA virus.
X. Zhang and D. L. Nuss (2008)
PNAS 105, 16749-16754
   Abstract »    Full Text »    PDF »
Hypovirus Papain-Like Protease p48 Is Required for Initiation but Not for Maintenance of Virus RNA Propagation in the Chestnut Blight Fungus Cryphonectria parasitica.
F. Deng and D. L. Nuss (2008)
J. Virol. 82, 6369-6378
   Abstract »    Full Text »    PDF »
Characterization of Hypovirus-Derived Small RNAs Generated in the Chestnut Blight Fungus by an Inducible DCL-2-Dependent Pathway.
X. Zhang, G. C. Segers, Q. Sun, F. Deng, and D. L. Nuss (2008)
J. Virol. 82, 2613-2619
   Abstract »    Full Text »    PDF »
Evidence that RNA silencing functions as an antiviral defense mechanism in fungi.
G. C. Segers, X. Zhang, F. Deng, Q. Sun, and D. L. Nuss (2007)
PNAS 104, 12902-12906
   Abstract »    Full Text »    PDF »
Genome Sequence, Full-Length Infectious cDNA Clone, and Mapping of Viral Double-Stranded RNA Accumulation Determinant of Hypovirus CHV1-EP721.
H. Lin, X. Lan, H. Liao, T. B. Parsley, D. L. Nuss, and B. Chen (2007)
J. Virol. 81, 1813-1820
   Abstract »    Full Text »    PDF »
Synergism between a mycoreovirus and a hypovirus mediated by the papain-like protease p29 of the prototypic hypovirus CHV1-EP713.
L. Sun, D. L. Nuss, and N. Suzuki (2006)
J. Gen. Virol. 87, 3703-3714
   Abstract »    Full Text »    PDF »
Mycovirus Cryphonectria Hypovirus 1 Elements Cofractionate with trans-Golgi Network Membranes of the Fungal Host Cryphonectria parasitica..
D. Jacob-Wilk, M. Turina, and N. K. Van Alfen (2006)
J. Virol. 80, 6588-6596
   Abstract »    Full Text »    PDF »
Characterization of debilitation-associated mycovirus infecting the plant-pathogenic fungus Sclerotinia sclerotiorum.
J. Xie, D. Wei, D. Jiang, Y. Fu, G. Li, S. Ghabrial, and Y. Peng (2006)
J. Gen. Virol. 87, 241-249
   Abstract »    Full Text »    PDF »
A Reovirus of the Fungus Cryphonectria parasitica That Is Infectious as Particles and Related to the Coltivirus Genus of Animal Pathogens.
B. I. Hillman, S. Supyani, H. Kondo, and N. Suzuki (2004)
J. Virol. 78, 892-898
   Abstract »    Full Text »    PDF »
Hypovirus Papain-Like Protease p29 Functions in trans To Enhance Viral Double-Stranded RNA Accumulation and Vertical Transmission.
N. Suzuki, K. Maruyama, M. Moriyama, and D. L. Nuss (2003)
J. Virol. 77, 11697-11707
   Abstract »    Full Text »    PDF »
Transfection of Diaporthe perjuncta with Diaporthe RNA Virus.
N. Moleleki, S. W. van Heerden, M. J. Wingfield, B. D. Wingfield, and O. Preisig (2003)
Appl. Envir. Microbiol. 69, 3952-3956
   Abstract »    Full Text »    PDF »
Contribution of Protein p40 to Hypovirus-Mediated Modulation of Fungal Host Phenotype and Viral RNA Accumulation.
N. Suzuki and D. L. Nuss (2002)
J. Virol. 76, 7747-7759
   Abstract »    Full Text »    PDF »
Differential Modulation of Cellular Signaling Pathways by Mild and Severe Hypovirus Strains.
T. B. Parsley, B. Chen, L. M. Geletka, and D. L. Nuss (2002)
Eukaryot. Cell 1, 401-413
   Abstract »    Full Text »    PDF »
Double-Stranded RNA Mycovirus from Fusarium graminearum.
Y.-M. Chu, J.-J. Jeon, S.-J. Yea, Y.-H. Kim, S.-H. Yun, Y.-W. Lee, and K.-H. Kim (2002)
Appl. Envir. Microbiol. 68, 2529-2534
   Abstract »    Full Text »    PDF »
Using Chimeric Hypoviruses To Fine-Tune the Interaction between a Pathogenic Fungus and Its Plant Host.
B. Chen, L. M. Geletka, and D. L. Nuss (2000)
J. Virol. 74, 7562-7567
   Abstract »    Full Text »
Essential and Dispensable Virus-Encoded Replication Elements Revealed by Efforts To Develop Hypoviruses as Gene Expression Vectors.
N. Suzuki, L. M. Geletka, and D. L. Nuss (2000)
J. Virol. 74, 7568-7577
   Abstract »    Full Text »
Mapping of a Hypovirus p29 Protease Symptom Determinant Domain with Sequence Similarity to Potyvirus HC-Pro Protease.
N. Suzuki, B. Chen, and D. L. Nuss (1999)
J. Virol. 73, 9478-9484
   Abstract »    Full Text »    PDF »
Infectious cDNA Clone of Hypovirus CHV1-Euro7: a Comparative Virology Approach To Investigate Virus-Mediated Hypovirulence of the Chestnut Blight Fungus Cryphonectria parasitica.
B. Chen and D. L. Nuss (1999)
J. Virol. 73, 985-992
   Abstract »    Full Text »


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