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Science 3 June 1994:
Vol. 264. no. 5164, pp. 1458 - 1460
DOI: 10.1126/science.8197459
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Articles

Science, Vol 264, Issue 5164, 1458-1460
Copyright © 1994 by American Association for the Advancement of Science

articles

Tetrad analysis possible in Arabidopsis with mutation of the QUARTET (QRT) genes

D Preuss, SY Rhee, and RW Davis

Department of Biochemistry, Stanford University, CA 94305-5307.

Two Arabidopsis thaliana genes, QRT1 and QRT2, are required for pollen separation during normal development. In qrt mutants, the outer walls of the four meiotic products of the pollen mother cell are fused, and pollen grains are released in tetrads. Pollen is viable and fertile, and the cytoplasmic pollen contents are discrete. Pollination with a single tetrad usually yields four seeds, and genetic analysis confirmed that marker loci segregate in a 2:2 ratio within these tetrads. These mutations allow tetrad analysis to be performed in Arabidopsis and define steps in pollen cell wall development.


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Assaying genome-wide recombination and centromere functions with Arabidopsis tetrads.
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Development 124, 2645-2657
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Pollen tube guidance by the female gametophyte.
S. Ray, S. Park, and A Ray (1997)
Development 124, 2489-2498
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sidecar pollen, an Arabidopsis thaliana male gametophytic mutant with aberrant cell divisions during pollen development.
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Development 122, 3243-3253
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