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Science 13 May 1994:
Vol. 264. no. 5161, pp. 968 - 971
DOI: 10.1126/science.7909957
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Articles

Science, Vol 264, Issue 5161, 968-971
Copyright © 1994 by American Association for the Advancement of Science

articles

Enhancer point mutation results in a homeotic transformation in Drosophila

MJ Shimell, J Simon, W Bender, and MB O'Connor

Department of Molecular Biology and Biochemistry, University of California, Irvine 92717.

In Drosophila, the misexpression or altered activity of genes from the bithorax complex results in homeotic transformations. One of these genes, abd-A, normally specifies the identity of the second through fourth abdominal segments (A2 to A4). In the dominant Hyperabdominal mutations (Hab), portions of the third thoracic segment (T3) are transformed toward A2 as the result of ectopic abd-A expression. Sequence analysis and deoxyribonuclease I footprinting demonstrate that the misexpression of abd-A in two independent Hab mutations results from the same single base change in a binding site for the gap gene Kruppel protein. These results establish that the spatial limits of the homeotic genes are directly regulated by gap gene products.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Dissecting the regulatory landscape of the Abd-B gene of the bithorax complex.
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The ABC of the BX-C: the bithorax complex explained..
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Conservation of regulatory sequences and gene expression patterns in the disintegrating Drosophila Hox gene complex.
B. Negre, S. Casillas, M. Suzanne, E. Sanchez-Herrero, M. Akam, M. Nefedov, A. Barbadilla, P. de Jong, and A. Ruiz (2005)
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The Enhancer-Blocking Activity of the Fab-7 Boundary From the Drosophila Bithorax Complex Requires GAGA-Factor-Binding Sites.
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batman Interacts with Polycomb and trithorax Group Genes and Encodes a BTB/POZ Protein That Is Included in a Complex Containing GAGA Factor.
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Transcription defines the embryonic domains of cis-regulatory activity at the Drosophila bithorax complex.
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E. B. Lewis and the Bithorax Complex: Part II. From cis-trans Test to the Genetic Control of Development.
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Transcription through the iab-7 cis-regulatory domain of the bithorax complex interferes with maintenance of Polycomb-mediated silencing.
I. Hogga and F. Karch (2002)
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Transcription activates repressed domains in the Drosophila bithorax complex.
W. Bender and D. P. Fitzgerald (2002)
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The Trithorax-mimic Allele of Enhancer of zeste Renders Active Domains of Target Genes Accessible to Polycomb-Group-Dependent Silencing in Drosophila melanogaster.
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The iab-7 Polycomb Response Element Maps to a Nucleosome-Free Region of Chromatin and Requires Both GAGA and Pleiohomeotic for Silencing Activity.
R. K. Mishra, J. Mihaly, S. Barges, A. Spierer, F. Karch, K. Hagstrom, S. E. Schweinsberg, and P. Schedl (2001)
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Polycomb group proteins and heritable silencing of Drosophila Hox genes.
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A Drosophila ESC-E(Z) Protein Complex Is Distinct from Other Polycomb Group Complexes and Contains Covalently Modified ESC.
J. Ng, C. M. Hart, K. Morgan, and J. A. Simon (2000)
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Characterization of the transvection mediating region of the abdominal-B locus in Drosophila.
J Zhou, H Ashe, C Burks, and M Levine (1999)
Development 126, 3057-3065
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The Maize Regulatory Gene B-Peru Contains a DNA Rearrangement That Specifies Tissue-Specific Expression Through Both Positive and Negative Promoter Elements.
D. A. Selinger, D. Lisch, and V. L. Chandler (1998)
Genetics 149, 1125-1138
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D-Fos, a target gene of Decapentaplegic signalling with a critical role during Drosophila endoderm induction.
J Riese, G Tremml, and M Bienz (1997)
Development 124, 3353-3361
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In situ dissection of the Fab-7 region of the bithorax complex into a chromatin domain boundary and a Polycomb-response element.
J Mihaly, I Hogga, J Gausz, H Gyurkovics, and F Karch (1997)
Development 124, 1809-1820
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Fab-7 functions as a chromatin domain boundary to ensure proper segment specification by the Drosophila bithorax complex..
K Hagstrom, M Muller, and P Schedl (1996)
Genes & Dev. 10, 3202-3215
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Short-range transcriptional repressors mediate both quenching and direct repression within complex loci in Drosophila..
S Gray and M Levine (1996)
Genes & Dev. 10, 700-710
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A role for mel-18, a Polycomb group-related vertebrate gene, during theanteroposterior specification of the axial skeleton.
T Akasaka, M Kanno, R Balling, M. Mieza, M Taniguchi, and H Koseki (1996)
Development 122, 1513-1522
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Function of the Polycomb protein is conserved in mice and flies.
J Muller, S Gaunt, and P. Lawrence (1995)
Development 121, 2847-2852
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Discrete Polycomb-binding sites in each parasegmental domain of the bithorax complex.
A Chiang, M. O'Connor, R Paro, J Simon, and W Bender (1995)
Development 121, 1681-1689
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Regulation of the infraabdominal regions of the bithorax complex of Drosophila by gap genes.
F Casares and E Sanchez-Herrero (1995)
Development 121, 1855-1866
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