Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 13 May 1994:
Vol. 264. no. 5161, pp. 968 - 971
DOI: 10.1126/science.7909957
Prev | Table of Contents | Next

Articles

Science, Vol 264, Issue 5161, 968-971
Copyright © 1994 by American Association for the Advancement of Science

articles

Enhancer point mutation results in a homeotic transformation in Drosophila

MJ Shimell, J Simon, W Bender, and MB O'Connor

Department of Molecular Biology and Biochemistry, University of California, Irvine 92717.

In Drosophila, the misexpression or altered activity of genes from the bithorax complex results in homeotic transformations. One of these genes, abd-A, normally specifies the identity of the second through fourth abdominal segments (A2 to A4). In the dominant Hyperabdominal mutations (Hab), portions of the third thoracic segment (T3) are transformed toward A2 as the result of ectopic abd-A expression. Sequence analysis and deoxyribonuclease I footprinting demonstrate that the misexpression of abd-A in two independent Hab mutations results from the same single base change in a binding site for the gap gene Kruppel protein. These results establish that the spatial limits of the homeotic genes are directly regulated by gap gene products.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Dissecting the regulatory landscape of the Abd-B gene of the bithorax complex.
J. Mihaly, S. Barges, L. Sipos, R. Maeda, F. Cleard, I. Hogga, W. Bender, H. Gyurkovics, and F. Karch (2006)
Development 133, 2983-2993
   Abstract »    Full Text »    PDF »
The ABC of the BX-C: the bithorax complex explained..
R. K. Maeda and F. Karch (2006)
Development 133, 1413-1422
   Abstract »    Full Text »    PDF »
Conservation of regulatory sequences and gene expression patterns in the disintegrating Drosophila Hox gene complex.
B. Negre, S. Casillas, M. Suzanne, E. Sanchez-Herrero, M. Akam, M. Nefedov, A. Barbadilla, P. de Jong, and A. Ruiz (2005)
Genome Res. 15, 692-700
   Abstract »    Full Text »    PDF »
The Enhancer-Blocking Activity of the Fab-7 Boundary From the Drosophila Bithorax Complex Requires GAGA-Factor-Binding Sites.
S. Schweinsberg, K. Hagstrom, D. Gohl, P. Schedl, R. P. Kumar, R. Mishra, and F. Karch (2004)
Genetics 168, 1371-1384
   Abstract »    Full Text »    PDF »
Genetic and Phenotypic Analysis of Alleles of the Drosophila Chromosomal JIL-1 Kinase Reveals a Functional Requirement at Multiple Developmental Stages.
W. Zhang, Y. Jin, Y. Ji, J. Girton, J. Johansen, and K. M. Johansen (2003)
Genetics 165, 1341-1354
   Abstract »    Full Text »    PDF »
Activation of the knirps locus links patterning to morphogenesis of the second wing vein in Drosophila.
K. Lunde, J. L. Trimble, A. Guichard, K. A. Guss, U. Nauber, and E. Bier (2003)
Development 130, 235-248
   Abstract »    Full Text »    PDF »
batman Interacts with Polycomb and trithorax Group Genes and Encodes a BTB/POZ Protein That Is Included in a Complex Containing GAGA Factor.
M. Faucheux, J.-Y. Roignant, S. Netter, J. Charollais, C. Antoniewski, and L. Theodore (2003)
Mol. Cell. Biol. 23, 1181-1195
   Abstract »    Full Text »    PDF »
Transcription defines the embryonic domains of cis-regulatory activity at the Drosophila bithorax complex.
R. A. Drewell, E. Bae, J. Burr, and E. B. Lewis (2002)
PNAS 99, 16853-16858
   Abstract »    Full Text »    PDF »
E. B. Lewis and the Bithorax Complex: Part II. From cis-trans Test to the Genetic Control of Development.
I. Duncan and G. Montgomery (2002)
Genetics 161, 1-10
   Full Text »    PDF »
Transcription through the iab-7 cis-regulatory domain of the bithorax complex interferes with maintenance of Polycomb-mediated silencing.
I. Hogga and F. Karch (2002)
Development 129, 4915-4922
   Abstract »    Full Text »    PDF »
Transcription activates repressed domains in the Drosophila bithorax complex.
W. Bender and D. P. Fitzgerald (2002)
Development 129, 4923-4930
   Abstract »    Full Text »    PDF »
The Trithorax-mimic Allele of Enhancer of zeste Renders Active Domains of Target Genes Accessible to Polycomb-Group-Dependent Silencing in Drosophila melanogaster.
I. Bajusz, L. Sipos, Z. Gyorgypal, E. A. Carrington, R. S. Jones, J. Gausz, and H. Gyurkovics (2001)
Genetics 159, 1135-1150
   Abstract »    Full Text »    PDF »
The iab-7 Polycomb Response Element Maps to a Nucleosome-Free Region of Chromatin and Requires Both GAGA and Pleiohomeotic for Silencing Activity.
R. K. Mishra, J. Mihaly, S. Barges, A. Spierer, F. Karch, K. Hagstrom, S. E. Schweinsberg, and P. Schedl (2001)
Mol. Cell. Biol. 21, 1311-1318
   Abstract »    Full Text »
Polycomb group proteins and heritable silencing of Drosophila Hox genes.
D Beuchle, G Struhl, and J Muller (2001)
Development 128, 993-1004
   Abstract »    PDF »
A Drosophila ESC-E(Z) Protein Complex Is Distinct from Other Polycomb Group Complexes and Contains Covalently Modified ESC.
J. Ng, C. M. Hart, K. Morgan, and J. A. Simon (2000)
Mol. Cell. Biol. 20, 3069-3078
   Abstract »    Full Text »
Characterization of the transvection mediating region of the abdominal-B locus in Drosophila.
J Zhou, H Ashe, C Burks, and M Levine (1999)
Development 126, 3057-3065
   Abstract »    PDF »
The Maize Regulatory Gene B-Peru Contains a DNA Rearrangement That Specifies Tissue-Specific Expression Through Both Positive and Negative Promoter Elements.
D. A. Selinger, D. Lisch, and V. L. Chandler (1998)
Genetics 149, 1125-1138
   Abstract »    Full Text »    PDF »
D-Fos, a target gene of Decapentaplegic signalling with a critical role during Drosophila endoderm induction.
J Riese, G Tremml, and M Bienz (1997)
Development 124, 3353-3361
   Abstract »    PDF »
In situ dissection of the Fab-7 region of the bithorax complex into a chromatin domain boundary and a Polycomb-response element.
J Mihaly, I Hogga, J Gausz, H Gyurkovics, and F Karch (1997)
Development 124, 1809-1820
   Abstract »    PDF »
Fab-7 functions as a chromatin domain boundary to ensure proper segment specification by the Drosophila bithorax complex..
K Hagstrom, M Muller, and P Schedl (1996)
Genes & Dev. 10, 3202-3215
   Abstract »    PDF »
Short-range transcriptional repressors mediate both quenching and direct repression within complex loci in Drosophila..
S Gray and M Levine (1996)
Genes & Dev. 10, 700-710
   Abstract »    PDF »
A role for mel-18, a Polycomb group-related vertebrate gene, during theanteroposterior specification of the axial skeleton.
T Akasaka, M Kanno, R Balling, M. Mieza, M Taniguchi, and H Koseki (1996)
Development 122, 1513-1522
   Abstract »    PDF »
Function of the Polycomb protein is conserved in mice and flies.
J Muller, S Gaunt, and P. Lawrence (1995)
Development 121, 2847-2852
   Abstract »    PDF »
Discrete Polycomb-binding sites in each parasegmental domain of the bithorax complex.
A Chiang, M. O'Connor, R Paro, J Simon, and W Bender (1995)
Development 121, 1681-1689
   Abstract »    PDF »
Regulation of the infraabdominal regions of the bithorax complex of Drosophila by gap genes.
F Casares and E Sanchez-Herrero (1995)
Development 121, 1855-1866
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)