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Science 13 May 1994:
Vol. 264. no. 5161, pp. 933 - 941
DOI: 10.1126/science.8178153
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Articles

Science, Vol 264, Issue 5161, 933-941
Copyright © 1994 by American Association for the Advancement of Science

articles

Drosophila TAFII150: similarity to yeast gene TSM-1 and specific binding to core promoter DNA

CP Verrijzer, K Yokomori, JL Chen, and R Tjian

Howard Hughes Medical Institute, University of California, Berkeley 94720-3202.

In Drosophila and human cells, the TATA binding protein (TBP) of the transcription factor IID (TFIID) complex is tightly associated with multiple subunits termed TBP-associated factors (TAFs) that are essential for mediating regulation of RNA polymerase II transcription. The Drosophila TAFII150 has now been molecularly cloned and biochemically characterized. The deduced primary amino acid sequence of dTAFII150 reveals a striking similarity to the essential yeast gene, TSM-1. Furthermore, like dTAFII150, the TSM-1 protein is found associated with the TBP in vivo, thus identifying the first yeast homolog of a TAF associated with TFIID. Both the product of TSM-1 and dTAFII150 bind directly to TBP and dTAFII250, demonstrating a functional similarity between human and yeast TAFs. Surprisingly, DNA binding studies indicate that purified recombinant dTAFII150 binds specifically to DNA sequences overlapping the start site of transcription. The data demonstrate that at least one of the TAFs is a sequence-specific DNA binding protein and that dTAFII150 together with TBP are responsible for TFIID interactions with an extended region of the core promoter.


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   Abstract »    Full Text »    PDF »
CIF, an essential cofactor for TFIID-dependent initiator function..
J Kaufmann, C P Verrijzer, J Shao, and S T Smale (1996)
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   Abstract »    PDF »
The Histone Folds in Transcription Factor TFIID.
Y. Nakatani, S. Bagby, and M. Ikura (1996)
J. Biol. Chem. 271, 6575-6578
   Abstract »    Full Text »    PDF »
Drosophila TFIID binds to a conserved downstream basal promoter element that is present in many TATA-box-deficient promoters..
T W Burke and J T Kadonaga (1996)
Genes & Dev. 10, 711-724
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Alternative outcomes in assembly of promoter complexes: the roles of TBP and a flexible linker in placing TFIIIB on tRNA genes..
C A Joazeiro, G A Kassavetis, and E P Geiduschek (1996)
Genes & Dev. 10, 725-739
   Abstract »    PDF »
Epigenetic effects on yeast transcription caused by mutations in an actin-related protein present in the nucleus..
Y W Jiang and D J Stillman (1996)
Genes & Dev. 10, 604-619
   Abstract »    PDF »
Multiple TAFIIs Directing Synergistic Activation of Transcription.
F. Sauer, S. K. Hansen, and R. Tjian (1995)
Science 270, 1783-1788
   Abstract »    PDF »
The symmetry of the yeast U6 RNA gene's TATA box and the orientation of the TATA-binding protein in yeast TFIIIB..
S K Whitehall, G A Kassavetis, and E P Geiduschek (1995)
Genes & Dev. 9, 2974-2985
   Abstract »    PDF »
Human TAFII250 interacts with RAP74: implications for RNA polymerase II initiation..
S Ruppert and R Tjian (1995)
Genes & Dev. 9, 2747-2755
   Abstract »    PDF »
Evidence for Functional Binding and Stable Sliding of the TATA Binding Protein on Nonspecific DNA.
R. A. Coleman and B. F. Pugh (1995)
J. Biol. Chem. 270, 13850-13859
   Abstract »    Full Text »    PDF »
Reconstitution of transcription factor SL1: exclusive binding of TBP by SL1 or TFIID subunits.
L Comai, J. Zomerdijk, H Beckmann, S Zhou, A Admon, and R Tjian (1994)
Science 266, 1966-1972
   Abstract »    PDF »
Location, structure, and function of the target of a transcriptional activator protein..
H Tang, K Severinov, A Goldfarb, D Fenyo, B Chait, and R H Ebright (1994)
Genes & Dev. 8, 3058-3067
   Abstract »    PDF »
Multiple regions of TBP participate in the response to transcriptional activators in vivo..
W P Tansey, S Ruppert, R Tjian, and W Herr (1994)
Genes & Dev. 8, 2756-2769
   Abstract »    PDF »
Molecular cloning of the small (gamma) subunit of human TFIIA reveals functions critical for activated transcription..
J Ozer, P A Moore, A H Bolden, A Lee, C A Rosen, and P M Lieberman (1994)
Genes & Dev. 8, 2324-2335
   Abstract »    PDF »
Reconstitution of human TFIIA activity from recombinant polypeptides: a role in TFIID-mediated transcription..
X Sun, D Ma, M Sheldon, K Yeung, and D Reinberg (1994)
Genes & Dev. 8, 2336-2348
   Abstract »    PDF »
RRN6 and RRN7 encode subunits of a multiprotein complex essential for the initiation of rDNA transcription by RNA polymerase I in Saccharomyces cerevisiae..
D A Keys, L Vu, J S Steffan, J A Dodd, R T Yamamoto, Y Nogi, and M Nomura (1994)
Genes & Dev. 8, 2349-2362
   Abstract »    PDF »
The Intronless and TATA-less Human TAFII55 Gene Contains a Functional Initiator and a Downstream Promoter Element.
T. Zhou and CM Chiang (2001)
J. Biol. Chem. 276, 25503-25511
   Abstract »    Full Text »    PDF »
A Composite Nuclear Export Signal in the TBP-associated Factor TAFII105.
A. Rashevsky-Finkel, A. Silkov, and R. Dikstein (2001)
J. Biol. Chem. 276, 44963-44969
   Abstract »    Full Text »    PDF »
A downstream element in the human beta -globin promoter: Evidence of extended sequence-specific transcription factor IID contacts.
B. A. Lewis, T.-K. Kim, and S. H. Orkin (2000)
PNAS 97, 7172-7177
   Abstract »    Full Text »    PDF »


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Science. ISSN 0036-8075 (print), 1095-9203 (online)