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Science 8 April 1994:
Vol. 264. no. 5156, pp. 273 - 276
DOI: 10.1126/science.8146662
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Articles

Science, Vol 264, Issue 5156, 273-276
Copyright © 1994 by American Association for the Advancement of Science

articles

Requirement for the yeast gene LON in intramitochondrial proteolysis and maintenance of respiration

CK Suzuki, K Suda, N Wang, and G Schatz

Biozentrum der Universitat Basel, Switzerland.

The role of protein degradation in mitochondrial homeostasis was explored by cloning of a gene from Saccharomyces cerevisiae that encodes a protein resembling the adenosine triphosphate (ATP)-dependent bacterial protease Lon. The predicted yeast protein has a typical mitochondrial matrix-targeting sequence at its amino terminus. Yeast cells lacking a functional LON gene contained a nonfunctional mitochondrial genome, were respiratory-deficient, and lacked an ATP-dependent proteolytic activity present in the mitochondria of Lon+ cells. Lon- cells were also impaired in their ability to catalyze the energy-dependent degradation of several mitochondrial matrix proteins and they accumulated electron-dense inclusions in their mitochondrial matrix.


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Higher Plant Mitochondria.
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The ATPase and protease domains of yeast mitochondrial Lon: Roles in proteolysis and respiration-dependent growth.
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Bacterial Protease Lon Is a Site-specific DNA-binding Protein.
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Substitution of PIM1 Protease in Mitochondria by Escherichia coli Lon Protease.
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Degradation of Cytochrome Oxidase Subunits in Mutants of Yeast Lacking Cytochrome c and Suppression of the Degradation by Mutation of yme1.
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Selective, Energy-dependent Proteolysis in Escherichia coli.
S. Gottesman, S. Wickner, Y. Yubete, S.K. Singh, M. Kessel, and M. Maurizi (1995)
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   Abstract »    PDF »


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