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Science 18 March 1994:
Vol. 263. no. 5153, pp. 1618 - 1623
DOI: 10.1126/science.7907431

Articles

Science, Vol 263, Issue 5153, 1618-1623
Copyright © 1994 by American Association for the Advancement of Science


articles

Requirement for BDNF in activity-dependent survival of cortical neurons

A Ghosh, J Carnahan, and ME Greenberg

Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115.

Cultured embryonic cortical neurons from rats were used to explore mechanisms of activity-dependent neuronal survival. Cell survival was increased by the activation of voltage-sensitive calcium channels (VSCCs) but not by activation of N-methyl-D-aspartate receptors. These effects correlated with the expression of brain-derived neurotrophic factor (BDNF) induced by these two classes of calcium channels. Antibodies to BDNF (which block intracellular signaling by BDNF, but not by nerve growth factor, NT3, or NT4/5) reduced the survival of cortical neurons and reversed the VSCC-mediated increase in survival. Thus, endogenous BDNF is a trophic factor for cortical neurons whose expression is VSCC-regulated and that functions in the VSCC-dependent survival of these neurons.


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Anomalous L-Type Calcium Channels of Rat Spinal Motoneurons.
B. Hivert, S. Luvisetto, A. Navangione, A. Tottene, and D. Pietrobon (1999)
J. Gen. Physiol. 113, 679-694
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Molecular Correlates of Topographic Reorganization in Primary Visual Cortex Following Retinal Lesions.
S. Obata, J. Obata, A. Das, and C. D. Gilbert (1999)
Cereb Cortex 9, 238-248
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Thromboembolic Events Predispose the Brain to Widespread Cerebral Infarction After Delayed Transient Global Ischemia in Rats • Editorial Comment.
W. D. Dietrich, G. Danton, A. C. Hopkins, R. Prado, and W. Pearce (1999)
Stroke 30, 855-862
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BDNF Is a Target-Derived Survival Factor for Arterial Baroreceptor and Chemoafferent Primary Sensory Neurons.
R. Brady, S. I. A. Zaidi, C. Mayer, and D. M. Katz (1999)
J. Neurosci. 19, 2131-2142
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Suppression of Postischemic Hippocampal Nerve Growth Factor Expression by a c-fos Antisense Oligodeoxynucleotide.
J.-K. Cui, C. Y. Hsu, and P. K. Liu (1999)
J. Neurosci. 19, 1335-1344
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The Survival-Promoting Effect of Glial Cell Line-Derived Neurotrophic Factor on Axotomized Corticospinal Neurons In Vivo Is Mediated by an Endogenous Brain-Derived Neurotrophic Factor Mechanism.
K. M. Giehl, A. Schutte, P. Mestres, and Q. Yan (1998)
J. Neurosci. 18, 7351-7360
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Neurotrophins induce release of neurotrophins by the regulated secretory pathway.
A. Kruttgen, J. C. Moller, J. V. Heymach Jr., and E. M. Shooter (1998)
PNAS 95, 9614-9619
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Granule Neuron Regulation of Purkinje Cell Development: Striking a Balance Between Neurotrophin and Glutamate Signaling.
M. E. Morrison and C. A. Mason (1998)
J. Neurosci. 18, 3563-3573
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Functional Evidence that BDNF Is an Anterograde Neuronal Trophic Factor in the CNS.
J. P. Fawcett, S. X. Bamji, C. G. Causing, R. Aloyz, A. R. Ase, T. A. Reader, J. H. McLean, and F. D. Miller (1998)
J. Neurosci. 18, 2808-2821
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N-Shc and Sck, Two Neuronally Expressed Shc Adapter Homologs. THEIR DIFFERENTIAL REGIONAL EXPRESSION IN THE BRAIN AND ROLES IN NEUROTROPHIN AND Src SIGNALING.
T. Nakamura, S. Muraoka, R. Sanokawa, and N. Mori (1998)
J. Biol. Chem. 273, 6960-6967
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Calmodulin Is Involved in Membrane Depolarization-Mediated Survival of Motoneurons by Phosphatidylinositol-3 Kinase- and MAPK-Independent Pathways.
R. M. Soler, J. Egea, G. M. Mintenig, C. Sanz-Rodriguez, M. Iglesias, and J. X. Comella (1998)
J. Neurosci. 18, 1230-1239
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Acute and Chronic Increases in Excitability in Rat Hippocampal Slices After Perinatal Hypoxia In Vivo.
F. E. Jensen, C. Wang, C. E. Stafstrom, Z. Liu, C. Geary, and M. C. Stevens (1998)
J Neurophysiol 79, 73-81
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Retrograde Signaling in the Development and Modification of Synapses.
R. M. FITZSIMONDS and M.-M. POO (1998)
Physiol Rev 78, 143-170
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Hyperexcitability in Combined Entorhinal/Hippocampal Slices of Adult Rat After Exposure to Brain-Derived Neurotrophic Factor.
H. E. Scharfman (1997)
J Neurophysiol 78, 1082-1095
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A Molecular and Cellular Theory of Depression.
R. S. Duman, G. R. Heninger, and E. J. Nestler (1997)
Arch Gen Psychiatry 54, 597-606
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