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Science 18 March 1994: Vol. 263. no. 5153, pp. 1618 - 1623 DOI: 10.1126/science.7907431
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Articles
Science, Vol 263, Issue 5153, 1618-1623
Copyright © 1994 by American Association for the Advancement of Science
Requirement for BDNF in activity-dependent survival of cortical neurons
A Ghosh,
J Carnahan,
and
ME Greenberg
Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115.
Cultured embryonic cortical neurons from rats were used to explore mechanisms of activity-dependent neuronal survival. Cell survival was increased by the activation of voltage-sensitive calcium channels (VSCCs) but not by activation of N-methyl-D-aspartate receptors. These effects correlated with the expression of brain-derived neurotrophic factor (BDNF) induced by these two classes of calcium channels. Antibodies to BDNF (which block intracellular signaling by BDNF, but not by nerve growth factor, NT3, or NT4/5) reduced the survival of cortical neurons and reversed the VSCC-mediated increase in survival. Thus, endogenous BDNF is a trophic factor for cortical neurons whose expression is VSCC-regulated and that functions in the VSCC-dependent survival of these neurons.
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- Brain-derived Neurotrophic Factor Promotes Survival and Chemoprotection of Human Neuroblastoma Cells.
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- Brain-Derived Neurotrophic Factor Mediates the Anti-Apoptotic Effect of NMDA in Cerebellar Granule Neurons: Signal Transduction Cascades and Site of Ethanol Action.
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