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Science 11 March 1994:
Vol. 263. no. 5152, pp. 1438 - 1440
DOI: 10.1126/science.8128226
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Articles

Science, Vol 263, Issue 5152, 1438-1440
Copyright © 1994 by American Association for the Advancement of Science

articles

DNA repair rates mapped along the human PGK1 gene at nucleotide resolution

S Gao, R Drouin, and GP Holmquist

Beckman Research Institute of the City of Hope, Department of Biology, Duarte, CA 91010.

The repair of cyclobutane pyrimidine dimers (CPDs), DNA lesions induced by ultraviolet light, was studied at nucleotide resolution. Human fibroblasts were irradiated with ultraviolet light and allowed to repair. The DNA was enzymatically cleaved at the CPDs, and the induced breaks along the promoter and exon 1 of the PGK1 gene were mapped by ligation-mediated polymerase chain reaction. Repair rates within the nontranscribed strand varied as much as 15-fold, depending on nucleotide position. Preferential repair of the transcribed strand began just downstream of the transcription start site but was most pronounced beginning at nucleotide +140 in exon 1. The promoter contained two slowly repaired regions that coincided with two transcription factor binding sites.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
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Binding of Zinc Finger Protein Transcription Factor IIIA to Its Cognate DNA Sequence with Single UV Photoproducts at Specific Sites and Its Effect on DNA Repair.
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Nucleotide Sequence Context Effect of a Cyclobutane Pyrimidine Dimer upon RNA Polymerase II Transcription.
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Sequence-specific and Domain-specific DNA Repair in Xeroderma Pigmentosum and Cockayne Syndrome Cells.
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Science 263, 1374
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Slow repair of pyrimidine dimers at p53 mutation hotspots in skin cancer.
S Tornaletti and G. Pfeifer (1994)
Science 263, 1436-1438
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