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Science 11 March 1994:
Vol. 263. no. 5152, pp. 1423 - 1425
DOI: 10.1126/science.8128222
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Articles

Science, Vol 263, Issue 5152, 1423-1425
Copyright © 1994 by American Association for the Advancement of Science

articles

Recombination between viral RNA and transgenic plant transcripts

AE Greene and RF Allison

Department of Botany and Plant Pathology, Michigan State University, East Lansing 48824-1312.

Transformed plants expressing the 3' two-thirds of the cowpea chlorotic mottle virus (CCMV) capsid gene were inoculated with a CCMV deletion mutant lacking the 3' one-third of the capsid gene. Although the deletion inoculum replicates in inoculated cells, systemic infections occur only if recombination restores a functional capsid gene. Four of 125 inoculated transgenic plants, representing three different transgenic lines, became systemically infected. Analysis of viral RNA confirmed that RNA recombination had united the transgenic messenger RNA and the challenging virus through aberrant homologous recombination.


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