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Science 11 March 1994:
Vol. 263. no. 5152, pp. 1419 - 1421
DOI: 10.1126/science.263.5152.1419
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Articles

Cytosolic pH Gradients Associated with Tip Growth

Bryan C. Gibbon 1 and Darryl L. Kropf 1

1 Department of Biology, University of Utah, Salt Lake City, UT 84112, USA.

The presence of a cytosolic pH gradient and its relation to polar tip growth was investigated in rhizoid cells of Pelvetia embryos with the use of pH-sensitive microelectrodes and by ratio imaging. Growing rhizoid cells generated a longitudinal pH gradient in which the apical cytosol was 0.3 to 0.5 units more acidic than the cytosol at the base of the cell. Treatment with a membrane-permeant weak acid, propionic acid, dissipated the cytosolic pH gradient and inhibited growth. The magnitude of the pH gradient correlated well with the rate of tip elongation. The pH gradient spatially superimposed on the cytosolic calcium gradient, and inhibition of calcium fluxes by treatment with lanthanum abolished the pH gradient and inhibited growth.

Submitted on August 10, 1993
Accepted on November 19, 1993


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Science. ISSN 0036-8075 (print), 1095-9203 (online)