Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 28 January 1994:
Vol. 263. no. 5146, pp. 518 - 520
DOI: 10.1126/science.7507262
Prev | Table of Contents | Next

Articles

Science, Vol 263, Issue 5146, 518-520
Copyright © 1994 by American Association for the Advancement of Science

articles

Effective tumor vaccine generated by fusion of hepatoma cells with activated B cells

Y Guo, M Wu, H Chen, X Wang, G Liu, G Li, J Ma, and MS Sy

Tumor Immunology and Biotherapy Center, Eastern Institute of Hepatobiliary Surgery, Shanghai, China.

Fusion of BERH-2 rat hepatocellular carcinoma cells with activated B cells produced hybrid cells that lost their tumorigenicity and became immunogenic. Syngeneic rats injected with BERH-2-B hybrid cells became resistant to challenge with parental BERH-2 cells, and rats with established BERH-2 hepatomas were cured by subsequent injection of BERH-2-B cells. Both CD4+ and CD8+ cells were essential for the induction of protective immunity; however, only CD8+ cells were required for the eradication of BERH-2 tumors. The generation of hybrid tumor cells that elicit antitumor immune responses may be a useful strategy for cancer immunotherapy.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Vaccination of Dendritic Cells Loaded with Interleukin-12-Secreting Cancer Cells Augments In vivo Antitumor Immunity: Characteristics of Syngeneic and Allogeneic Antigen-Presenting Cell Cancer Hybrid Cells.
T. Suzuki, T. Fukuhara, M. Tanaka, A. Nakamura, K. Akiyama, T. Sakakibara, D. Koinuma, T. Kikuchi, R. Tazawa, M. Maemondo, et al. (2005)
Clin. Cancer Res. 11, 58-66
   Abstract »    Full Text »    PDF »
T cell immunity using transgenic B lymphocytes.
M. Gerloni, M. Rizzi, P. Castiglioni, and M. Zanetti (2004)
PNAS 101, 3892-3897
   Abstract »    Full Text »    PDF »
Enhancing the Efficacy of a Weak Allogeneic Melanoma Vaccine by Viral Fusogenic Membrane Glycoprotein-mediated Tumor Cell-Tumor Cell Fusion.
E. Linardakis, A. Bateman, V. Phan, A. Ahmed, M. Gough, K. Olivier, R. Kennedy, F. Errington, K. J. Harrington, A. Melcher, et al. (2002)
Cancer Res. 62, 5495-5504
   Abstract »    Full Text »    PDF »
Generation of potent Th1 responses from patients with lymphoid malignancies after differentiation of B lymphocytes into dendritic-like cells.
M. Mohty, D. Isnardon, A. Charbonnier, M. Lafage-Pochitaloff, M. Merlin, D. Sainty, D. Olive, and B. Gaugler (2002)
Int. Immunol. 14, 741-750
   Abstract »    Full Text »    PDF »
Human glioma cells transformed by IGF-I triple helix technology show immune and apoptotic characteristics determining cell selection for gene therapy of glioblastoma.
A Ly, H T Duc, M Kalamarides, L A Trojan, Y Pan, A Shevelev, J-C Francois, T Noel, A Kane, D Henin, et al. (2001)
Mol. Pathol. 54, 230-239
   Abstract »    Full Text »    PDF »
Blood Level of B and CD4+ Lymphocytes Measured before Induction of an Experimental Tumor in Rats Predicts Tumor Progression and Survival.
M. K. Demetrikopoulos, R. H. Goldfarb, Z.-b. Zhang, and J. M. Weiss (2000)
Cancer Epidemiol. Biomarkers Prev. 9, 609-617
   Abstract »    Full Text »
CD34+ Acute Myeloid and Lymphoid Leukemic Blasts Can Be Induced to Differentiate Into Dendritic Cells.
A. Cignetti, E. Bryant, B. Allione, A. Vitale, R. Foa, and M.A. Cheever (1999)
Blood 94, 2048-2055
   Abstract »    Full Text »    PDF »
Protein Transfer of Glycosyl-phosphatidylinositol-B7-1 into Tumor Cell Membranes: A Novel Approach to Tumor Immunotherapy.
R. S. McHugh, S. Nagarajan, Y.-C. Wang, K. W. Sell, and P. Selvaraj (1999)
Cancer Res. 59, 2433-2437
   Abstract »    Full Text »    PDF »
Evidence for Involvement of B Lymphocytes in the Surveillance of Lung Metastasis in the Rat.
N. Quan, Z.-b. Zhang, M. K. Demetrikopoulos, R. P. Kitson, W. H. Chambers, R. H. Goldfarb, and J. M. Weiss (1999)
Cancer Res. 59, 1080-1089
   Abstract »    Full Text »    PDF »
Eliciting T Cell Immunity Against Poorly Immunogenic Tumors by Immunization with Dendritic Cell-Tumor Fusion Vaccines.
J. Wang, S. Saffold, X. Cao, J. Krauss, and W. Chen (1998)
J. Immunol. 161, 5516-5524
   Abstract »    Full Text »    PDF »
Cutting Edge: Physical Interaction Between Dendritic Cells and Tumor Cells Results in an Immunogen That Induces Protective and Therapeutic Tumor Rejection.
C. M. Celluzzi and L. D. Falo Jr. (1998)
J. Immunol. 160, 3081-3085
   Abstract »    Full Text »    PDF »
Autologous Tumor Infiltrating T Cells Cytotoxic for Follicular Lymphoma Cells Can Be Expanded In Vitro.
J. L. Schultze, M. J. Seamon, S. Michalak, J. G. Gribben, and L. M. Nadler (1997)
Blood 89, 3806-3816
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)