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Science 28 January 1994:
Vol. 263. no. 5146, pp. 515 - 518
DOI: 10.1126/science.8290959

Articles

Science, Vol 263, Issue 5146, 515-518
Copyright © 1994 by American Association for the Advancement of Science


articles

Meiosis-induced double-strand break sites determined by yeast chromatin structure

TC Wu and M Lichten

Laboratory of Biochemistry, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892.

Double-strand DNA breaks (DSBs) occur at recombination hotspots during Saccharomyces cerevisiae meiosis and are thought to initiate exchange at these loci. Analysis of DSB sites in three regions of the yeast genome indicated that breaks occur at or near many potential transcription promoters and that DSBs initiate most, if not all, meiotic recombination. DSB sites displayed deoxyribonuclease I hypersensitivity in chromatin from mitotic and meiotic cells, and changes in chromatin structure produced parallel changes in the occurrence of DSBs. Thus, features of chromatin structure that are established before meiosis play a role in determining where meiotic recombination events initiate.


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Meiosis and the Evolution of Recombination at Low Mutation Rates.
D. D. G. Gessler and S. Xu (2000)
Genetics 156, 449-456
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E. S. Davis, B. K. Shafer, and J. N. Strathern (2000)
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J. S. Heslop-Harrison (2000)
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A. J. Jeffreys, A. Ritchie, and R. Neumann (2000)
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Saturation Mapping of a Gene-Rich Recombination Hot Spot Region in Wheat.
J. D. Faris, K. M. Haen, and B. S. Gill (2000)
Genetics 154, 823-835
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Mol. Cell. Biol. 19, 4832-4842
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F. Paques and J. E. Haber (1999)
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D. T. Kirkpatrick, Q. Fan, and T. D. Petes (1999)
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J. Murray, J. Buard, D. L. Neil, E. Yeramian, K. Tamaki, C. Hollies, and A. J. Jeffreys (1999)
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N. Kon, S. C. Schroeder, M. D. Krawchuk, and W. P. Wahls (1998)
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An In Vivo Topoisomerase II Cleavage Site and a DNase I Hypersensitive Site Colocalize Near Exon 9 in the MLL Breakpoint Cluster Region.
P. L. Strissel, R. Strick, J. D. Rowley, and N. J. Zeleznik-Le (1998)
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K. S. McKim and A. Hayashi-Hagihara (1998)
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Meiotic Chromosome Morphology and Behavior in zip1 Mutants of Saccharomyces cerevisiae.
K.-S. Tung and G. S. Roeder (1998)
Genetics 149, 817-832
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DNA annealing by Rad52 Protein is stimulated by specific interaction with the complex of replication protein A and single-stranded DNA.
T. Sugiyama, J. H. New, and S. C. Kowalczykowski (1998)
PNAS 95, 6049-6054
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The Microsatellite Sequence (CT)n · (GA)n Promotes Stable Chromosomal Integration of Large Tandem Arrays of Functional Human U2 Small Nuclear RNA Genes.
A. D. Bailey, T. Pavelitz, and A. M. Weiner (1998)
Mol. Cell. Biol. 18, 2262-2271
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DNA-Binding Activities of Hop1 Protein, a Synaptonemal Complex Component from Saccharomyces cerevisiae.
K. M. Kironmai, K. Muniyappa, D. B. Friedman, N. M. Hollingsworth, and B. Byers (1998)
Mol. Cell. Biol. 18, 1424-1435
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Mutations in the MRE11, RAD50, XRS2, and MRE2 genes alter chromatin configuration at meiotic DNA double-stranded break sites in premeiotic and meiotic cells.
K. Ohta, A. Nicolas, M. Furuse, A. Nabetani, H. Ogawa, and T. Shibata (1998)
PNAS 95, 646-651
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Aspects of three-dimensional chromosome reorganization during the onset of human male meiotic prophase.
H Scherthan, R Eils, E Trelles-Sticken, S Dietzel, T Cremer, H Walt, and A Jauch (1998)
J. Cell Sci. 111, 2337-2351
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Relationship between transcription and initiation of meiotic recombination: Toward chromatin accessibility.
A. Nicolas (1998)
PNAS 95, 87-89
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Evidence for Independent Mismatch Repair Processing on Opposite Sides of a Double-Strand Break in Saccharomyces cerevisiae.
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Genetics 148, 59-70
Molecular Analysis of the Major MHC Recombinational Hot Spot Located Within the G7c Gene of the Murine Class III Region That Is Involved in Disease Susceptibility.
M. Snoek, C. Teuscher, and H. van Vugt (1998)
J. Immunol. 160, 266-272
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Transcription factor Mts1/Mts2 (Atf1/Pcr1, Gad7/Pcr1) activates the M26 meiotic recombination hotspot in pombe.
N. Kon, M. D. Krawchuk, B. G. Warren, G. R. Smith, and W. P. Wahls (1997)
PNAS 94, 13765-13770
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Meiotic chromosomes: it takes two to tango.
G. S. Roeder (1997)
Genes & Dev. 11, 2600-2621
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Cisplatin increases meiotic crossing-over in mice.
W. H. Hanneman, M. E. Legare, S. Sweeney, and J. C. Schimenti (1997)
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The hotspot conversion paradox and the evolution of meiotic recombination.
A. Boulton, R. S. Myers, and R. J. Redfield (1997)
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Position- and orientation-independent activity of the Schizosaccharomyces pombe meiotic recombination hot spot M26.
M. E. Fox, J. B. Virgin, J. Metzger, and G. R. Smith (1997)
PNAS 94, 7446-7451
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Clustering of meiotic double-strand breaks on yeast chromosome III.
F. Baudat and A. Nicolas (1997)
PNAS 94, 5213-5218
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Genes & Dev. 11, 876-886
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Expression of Human RAD52 Confers Resistance to Ionizing Radiation in Mammalian Cells.
M. S. Park and M. S. Park (1995)
J. Biol. Chem. 270, 15467-15470
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A heteromeric protein that binds to a meiotic homologous recombination hot spot: correlation of binding and hot spot activity..
W P Wahls and G R Smith (1994)
Genes & Dev. 8, 1693-1702
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Meiotic instability of CAG repeat tracts occurs by double-strand break repair in yeast.
C. Jankowski, F. Nasar, and D. K. Nag (2000)
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PNAS 99, 6157-6162
   Abstract »    Full Text »    PDF »
Meiotic recombination frequencies are affected by nutritional states in Saccharomycescerevisiae.
M. F. F. Abdullah and R. H. Borts (2001)
PNAS 98, 14524-14529
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Science. ISSN 0036-8075 (print), 1095-9203 (online)