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Science 21 January 1994:
Vol. 263. no. 5145, pp. 390 - 393
DOI: 10.1126/science.7904084
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Articles

Science, Vol 263, Issue 5145, 390-393
Copyright © 1994 by American Association for the Advancement of Science

articles

Cell membrane resealing by a vesicular mechanism similar to neurotransmitter release

RA Steinhardt, G Bi, and JM Alderton

Department of Molecular and Cell Biology, University of California, Berkeley 94720.

After injury to the cell membrane, rapid resealing of the membrane occurs with little loss of intracellular contents. This process has been studied by measurement of the rate of dye loss after membrane puncture in both the sea urchin embryo and 3T3 fibroblasts. Resealing of disrupted cell membranes requires external calcium that can be antagonized by magnesium. Block of multifunctional calcium/calmodulin kinase, which regulates exocytotic vesicle availability at synapses, and of kinesin, which is required for outward-directed transport of vesicles, inhibited membrane resealing. Resealing was also inhibited by botulinum neurotoxins B and A, suggesting that the two synaptosomal-associated proteins synaptobrevin and SNAP-25 also participate in resealing. This pattern of inhibition indicates that the calcium-dependent mechanisms for cell membrane resealing may involve vesicle delivery, docking, and fusion, similar to the exocytosis of neurotransmitters.


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   Abstract »    Full Text »    PDF »
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Endocytotic Formation of Vesicles and Other Membranous Structures Induced by Ca2+ and Axolemmal Injury.
C. S. Eddleman, M. L. Ballinger, M. E. Smyers, H. M. Fishman, and G. D. Bittner (1998)
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Reconstitution of Calcium-triggered Membrane Fusion Using "Reserve" Granules.
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Synaptic Transmission Deficits in Caenorhabditis elegans Synaptobrevin Mutants.
M. L. Nonet, O. Saifee, H. Zhao, J. B. Rand, and L. Wei (1998)
J. Neurosci. 18, 70-80
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Retrograde Signaling in the Development and Modification of Synapses.
R. M. FITZSIMONDS and M.-M. POO (1998)
Physiol Rev 78, 143-170
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Neuronal swelling and surface area regulation: elevated intracellular calcium is not a requirement.
T. L. Herring, I. M. Slotin, J. M. Baltz, and C. E. Morris (1998)
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Wound-induced calcium waves in alveolar type II cells.
L. E. Hinman, G. J. Beilman, K. E. Groehler, and P. J. Sammak (1997)
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M. Terasaki, K. Miyake, and P. L. McNeil (1997)
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Z. Wang, M. Tymianski, O. T. Jones, and M. Nedergaard (1997)
J. Neurosci. 17, 7359-7371
   Abstract »    Full Text »    PDF »
Kinesin- and Myosin-driven Steps of Vesicle Recruitment for Ca2+-regulated Exocytosis.
G.-Q. Bi, R. L. Morris, G. Liao, J. M. Alderton, J. M. Scholey, and R. A. Steinhardt (1997)
J. Cell Biol. 138, 999-1008
   Abstract »    Full Text »    PDF »
Heterotrimeric Kinesin-II Is Required for the Assembly of Motile 9+2 Ciliary Axonemes on Sea Urchin Embryos.
R. L. Morris and J. M. Scholey (1997)
J. Cell Biol. 138, 1009-1022
   Abstract »    Full Text »    PDF »
Ca2+-independent and Ca2+/GTP-binding protein-controlled exocytosis in a plant cell.
U. Homann and M. Tester (1997)
PNAS 94, 6565-6570
   Abstract »    Full Text »    PDF »
Second Messengers, Trafficking-Related Proteins, and Amino Acid Residues that Contribute to the Functional Regulation of the Rat Brain GABA Transporter GAT1.
M. W. Quick, J. L. Corey, N. Davidson, and H. A.  Lester (1997)
J. Neurosci. 17, 2967-2979
   Abstract »    Full Text »    PDF »
Repair of plasmalemmal lesions by vesicles.
C. S. Eddleman, M. L. Ballinger, M. E. Smyers, C. M. Godell, H. M. Fishman, and G. D. Bittner (1997)
PNAS 94, 4745-4750
   Abstract »    Full Text »    PDF »
Loss, Restoration, and Maintenance of Plasma Membrane Integrity.
P. L. McNeil and R. A. Steinhardt (1997)
J. Cell Biol. 137, 1-4
   Full Text »    PDF »
Lysosomes Behave as Ca2+-regulated Exocytic Vesicles in Fibroblasts and Epithelial Cells.
A. Rodriguez, P. Webster, J. Ortego, and N. W. Andrews (1997)
J. Cell Biol. 137, 93-104
   Abstract »    Full Text »    PDF »
In vitro exocytosis in sea urchin eggs requires a synaptobrevin-related protein.
J Avery, A Hodel, and M Whitaker (1997)
J. Cell Sci. 110, 1555-1561
   Abstract »    PDF »
How do injured cells communicate with the surviving cell monolayer?.
P. Sammak, L. Hinman, P. Tran, M. Sjaastad, and T. Machen (1997)
J. Cell Sci. 110, 465-475
   Abstract »    PDF »
Polarized Expression of GABA Transporters in Madin-Darby Canine Kidney Cells and Cultured Hippocampal Neurons.
J. Ahn, O. Mundigl, T. R. Muth, G. Rudnick, and M. J. Caplan (1996)
J. Biol. Chem. 271, 6917-6924
   Abstract »    Full Text »    PDF »
Calcium-independent regulation of pigment granule aggregation and dispersion in teleost retinal pigment epithelial cells.
C King-Smith, P Chen, D Garcia, H Rey, and B Burnside (1996)
J. Cell Sci. 109, 33-43
   Abstract »    PDF »
Contraction-Induced Cell Wounding and Release of Fibroblast Growth Factor in Heart.
M. S. F. Clarke, R. W. Caldwell, H. Chiao, K. Miyake, and P. L. McNeil (1995)
Circ. Res. 76, 927-934
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Kinectin, an essential anchor for kinesin-driven vesicle motility.
J Kumar, H Yu, and M. Sheetz (1995)
Science 267, 1834-1837
   Abstract »    PDF »


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