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Science 14 January 1994:
Vol. 263. no. 5144, pp. 224 - 227
DOI: 10.1126/science.8284672
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Articles

Science, Vol 263, Issue 5144, 224-227
Copyright © 1994 by American Association for the Advancement of Science

articles

Crystal structure of the DNA binding domain of the heat shock transcription factor

CJ Harrison, AA Bohm, and HC Nelson

Department of Molecular and Cell Biology, University of California, Berkeley 94720.

The structure of the DNA binding domain, determined at 1.8 angstrom resolution, contains a three-helix bundle that is capped by a four-stranded antiparallel beta sheet. This structure is a variant of the helix-turn-helix motif, typified by catabolite activator protein. In the heat shock transcription factor, the first helix of the motif (alpha 2) has an alpha-helical bulge and a proline-induced kink. The angle between the two helices of the motif (alpha 2 and alpha 3) is about 20 degrees smaller than the average for canonical helix-turn-helix proteins. Nevertheless, the relative positions of the first and third helices of the bundle (alpha 1 and alpha 3) are conserved. It is proposed here that the first helix of the three-helix bundle be considered a component of the helix-turn-helix motif.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
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The DNA-binding Domain of Yeast Heat Shock Transcription Factor Independently Regulates Both the N- and C-terminal Activation Domains.
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Physiol Rev 81, 1461-1497
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The loop domain of heat shock transcription factor 1 dictates DNA-binding specificity and responses to heat stress.
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Am J Physiol Cell Physiol 279, C1506-C1515
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The Skn7 Response Regulator of Saccharomyces cerevisiae Interacts with Hsf1 In Vivo and Is Required for the Induction of Heat Shock Genes by Oxidative Stress.
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The Yeast Heat Shock Transcription Factor Changes Conformation in Response to Superoxide and Temperature.
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Modulation of Human Heat Shock Factor Trimerization by the Linker Domain.
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Structural basis of DNA recognition by the heterodimeric cell cycle transcription factor E2F-DP.
N. Zheng, E. Fraenkel, C. O. Pabo, and N. P. Pavletich (1999)
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Heat Shock Element Architecture Is an Important Determinant in the Temperature and Transactivation Domain Requirements for Heat Shock Transcription Factor.
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Evidence That Partial Unwrapping of DNA from Nucleosomes Facilitates the Binding of Heat Shock Factor following DNA Replication in Yeast.
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The Tomato Hsf System: HsfA2 Needs Interaction with HsfA1 for Efficient Nuclear Import and May Be Localized in Cytoplasmic Heat Stress Granules.
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F. Pio, R. Kodandapani, C.-Z. Ni, W. Shepard, M. Klemsz, S. R. McKercher, R. A. Maki, and K. R. Ely (1996)
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Crystal Structure of S-Adenosylmethionine Synthetase.
F. Takusagawa, S. Kamitori, S. Misaki, and G. D. Markham (1996)
J. Biol. Chem. 271, 136-147
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Mutated Yeast Heat Shock Transcription Factor Exhibits Elevated Basal Transcriptional Activation and Confers Metal Resistance.
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Co-crystallization of an ETS Domain (PU.1) in Complex with DNA.
F.édér. Pio, C.-Z. Ni, R. S. Mitchell, J. Knight, S. McKercher, M. Klemsz, A. Lombardo, R. A. Maki, and K. R. Ely (1995)
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Complex Multigene Family of Functionally Distinct Hsp70s of Yeast.
E. Craig, T. Ziegelhoffer, J. Nelson, S. Laloraya, and J. Halladay (1995)
Cold Spring Harb Symp Quant Biol 60, 441-449
   Abstract »    PDF »
Creating Temperature-sensitive Winged Helix Transcription Factors. AMINO ACIDS THAT STABILIZE THE DNA BINDING DOMAIN OF HNF3.
K. Stevens, L. Cirillo, and K. S. Zaret (2000)
J. Biol. Chem. 275, 30471-30477
   Abstract »    Full Text »    PDF »


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