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Science 7 January 1994:
Vol. 263. no. 5143, pp. 98 - 101
DOI: 10.1126/science.7505952
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Articles

Science, Vol 263, Issue 5143, 98-101
Copyright © 1994 by American Association for the Advancement of Science

articles

The Met proto-oncogene mesenchymal to epithelial cell conversion

I Tsarfaty, S Rong, JH Resau, S Rulong, PP da Silva, and GF Vande Woude

ABL-Basic Research Program, National Cancer Institute (NCI)-Frederick Cancer Research and Development Center, MD 21702-1201.

Coexpression of the human Met receptor and its ligand, hepatocyte growth factor/scatter factor (HGF/SF), in NIH 3T3 fibroblasts causes the cells to become tumorigenic in nude mice. The resultant tumors display lumen-like morphology, contain carcinoma-like focal areas with intercellular junctions resembling desmosomes, and coexpress epithelial (cytokeratin) and mesenchymal (vimentin) cytoskeletal markers. The tumor cells also display enhanced expression of desmosomal and tight-junction proteins. The apparent mesenchymal to epithelial conversion of the tumor cells mimics the conversion that occurs during embryonic kidney development, suggesting that Met-HGF/SF signaling plays a role in this process as well as in tumors that express both epithelial and mesenchymal markers.


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Science. ISSN 0036-8075 (print), 1095-9203 (online)